Angelica pubescens (root) - Revision history

Admin: add askbox

2014-06-02T17:48:35Z

add askbox

Admin: new layout

2014-03-16T20:40:56Z

new layout

Staffer at 20:55, 15 March 2014

2014-03-15T20:55:20Z

Staffer at 20:53, 15 March 2014

2014-03-15T20:53:03Z

Admin at 19:06, 14 March 2014

2014-03-14T19:06:28Z

Admin at 19:06, 14 March 2014

2014-03-14T19:06:05Z

Staffer: Nomenclature updated

2014-03-13T16:13:17Z

Nomenclature updated

Admin at 15:34, 7 August 2013

2013-08-07T15:34:43Z

Admin: /* MOBOT */

2013-08-06T22:18:24Z

‎MOBOT

AHPAStaff: addition of hptlc info.--jd

2013-06-25T00:58:59Z

addition of hptlc info.--jd

New page

=Introduction=
[[Category:NoIntro]]

=Macroscopic Entries=
[[Category:NoMacroscopy]]

=Microscopic Entries=
[[Category:NoMicroscopy]]

=HPTLC Entries=
{{HPTLC | source=HPTLC Association
| companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
| companyURL=http://www.hptlc-association.org/
| mainimage=Angelica pubescens-UV 254 nm-hptclc-association.png
| caption1=Doubleteeth Pubescent Angelica (root) HPTLC ID - UV 254 nm
| description=Doubleteeth Pubescent Angelica (root) (''Angelica pubescens'')
| image2=Angelica pubescens-UV 366 nm-hptclc-association.png
| caption2=Doubleteeth Pubescent Angelica (root) HPTLC ID - UV 366 nm
| image3=Angelica pubescens-Sulfuric acid, WRT-hptclc-association.png
| caption3=Doubleteeth Pubescent Angelica (root) HPTLC ID - Sulfuric acid, WRT
|
| stationaryphase=Stationary phase, i.e. Silica gel 60, F254
| mobilephase=Toluene, ethyl acetate, glacial acetic acid 90:10:1 (v/v/v)
| prep=Sample: Mix 1.0 g of powdered sample with 4 mL of heptane and sonicate for 5 minutes, then centrifuge and filter the solutions and use the filtrates as test solutions.

Derivatization reagent: Sulfuric acid reagent, Preparation: 20 mL sulfuric acid with 180 mL methanol, Use: Dip for 1 s, heat at 100°C for 5 min.
| detection=Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33%
| referencesamples=Reference: Dissolve 1 mg each of osthole and imperatorin in 10 mL of methanol. Optional: Dissolve 1 mg of Z-ligustilide in 10 mL of methanol.
|
| lanes=Lanes, from left to right (Track, Volume, Sample):
# 4 µL Imperatorin, z-Ligustilide (incr. Rf)
# 4 µL Osthole
# 4 µL Angelica root 1 (these samples do not comply with Ph.Eur specifications)
# 4 µL Angelica root 2
# 4 µL Angelica root 3
# 4 µL Chinese Angelica root
# 4 µL Dahurian Angelica root
# '''4 µL Doubleteeth pubescent Angelica root 1'''
# '''4 µL Doubleteeth pubescent Angelica root 2'''
# 4 µL Lovage root 1 (these samples do not comply with Ph.Eur specifications)
# 4 µL Lovage root 2
# 4 µL Lovage root 3
# 4 µL Chinese lovage root (''Ligusticum sinensis'')
# 4 µL Chinese lovage root 1 (''Ligusticum jeholense'')
# 4 µL Chinese lovage root 2 (''Ligusticum jeholense'')
# 4 µL Chinese lovage root (''Ligusticum chuanxiong'')

| notes=''Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.''

System suitability test: Imperatorin: greenish fluorescent zone at Rf ~ 0.31 (UV 366 nm). Osthole: blue fluorescent zone at Rf ~ 0.36 (UV 366 nm).

Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. Under UV 254 nm the chromatogram of the test solution shows a quenching zone corresponding to reference osthole (Rf ~ 0.36) (red arrow). There may be a quenching zone corresponding to imperatorin (dashed red arrow) and there may be a quenching zone at the position of z-ligustilide (but no fluorescent zone). There are several weak quenching zones below these zones. Under UV 366 nm the chromatogram of the test solution shows a white blue fluorescent zone at Rf ~ 0.57 and a prominent blue fluorescent zone corresponding to osthole (yellow arrows). Above this zone there may be a very faint green whitish zone. There may be a green whitish fluorescent zone corresponding to imperatorin (dashed yellow arrow). Below this zone there is a prominent blue fluorescent zone and several weak bluish and greenish zones. After derivatization under white light there are two prominent purple zones below the solvent front. A purple zone corresponding to osthole is detected and there may be a purple zone corresponding to imperatorin. Below this zone there are several yellow and purple zones. NOTE: the two samples of Doubleteeth pubescent Angelica root show variation.

Test for adulteration: Under UV 254 nm no blue fluorescent zone is seen at Rf ~ 0.57. Under UV 366 nm no blue fluorescent zone just below the position of imperatorin is seen. After derivatization under white light no zone is seen at Rf ~ 0.57 (black arrow) (Angelica root, Chinese Angelica root, Dahurian Angelica root, Lovage root, Chinese lovage root).

| }}

=Other Points of Interest=

[[Category:Blank2]]

← Older revision		Revision as of 17:48, 2 June 2014
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−	{{DISPLAYTITLE:'' Angelica pubescens'' (root) }}	+	{{DISPLAYTITLE:'' Angelica pubescens'' (root) }} {{askbox\|herb=''Angelica pubescens''}}
	=Nomenclature=		=Nomenclature=

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 =Macroscopic Characteristics=
-{{Macroscopy | source=MOBOT, Tropicos.org
+{{Media |cat=Macroscopy | source=MOBOT, Tropicos.org
               | mainimage=Angelica pubescens Tropicos 100107413.jpg
               | companyimage=TropicosLogo.gif
               | companyURL=http://www.tropicos.org/Image/100107413
               | reference=Tropicos.org. Missouri Botanical Garden. 06 Aug 2013 <http://www.tropicos.org/Image/100107413>
               | }}

← Older revision		Revision as of 20:55, 15 March 2014
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	=Botanical Voucher Specimen=		=Botanical Voucher Specimen=
−
−	~~=Microscopic Characteristics=~~

	=Organoleptic Characteristics=		=Organoleptic Characteristics=

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 =Botanical Voucher Specimen=
-=Microscopic Entries=
+=Microscopic Characteristics=
 =Organoleptic Characteristics=

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 =Botanical Voucher Specimen=
 {{Macroscopy | source=MOBOT, Tropicos.org
@@ Line 22: / Line 28: @@
               | reference=Tropicos.org. Missouri Botanical Garden. 06 Aug 2013 <http://www.tropicos.org/Image/100107413>
               | }}
 =Microscopic Characteristics=

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 =Nomenclature=
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 |notes= }}
-=Macroscopic Entries=
+=Botanical Voucher Specimen=
 {{Macroscopy | source=MOBOT, Tropicos.org
               | mainimage=Angelica pubescens Tropicos 100107413.jpg
@@ Line 24: / Line 26: @@
 [[Category:NoMicroscopy]]
-=HPTLC Entries=
+=Organoleptic Characteristics=
 {{HPTLC | source=HPTLC Association
               | companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
@@ Line 72: / Line 81: @@
               | }}
-=Other Points of Interest=
+<references />
-−
-[[Category:Blank2]]

@@ Line 7: / Line 7: @@
               | companyimage=TropicosLogo.gif
               | companyURL=http://www.tropicos.org/Image/100107413
-              | caption1=Angelica (''Angelica pubescens'') Photo (general)
+              | caption1=Angelica (''Angelica pubescens'')
               | description=Angelica (''Angelica pubescens'')
               | reference=Tropicos.org. Missouri Botanical Garden. 06 Aug 2013 <http://www.tropicos.org/Image/100107413>