Artemisia annua (leaf) - Revision history

Staffer: Botanical Voucher Kew added

2014-08-18T22:33:00Z

Botanical Voucher Kew added

Admin: add askbox

2014-06-02T17:32:50Z

add askbox

Staffer: Voucher layout updated

2014-03-21T03:08:06Z

Voucher layout updated

Admin at 19:38, 15 March 2014

2014-03-15T19:38:04Z

Admin at 15:01, 15 March 2014

2014-03-15T15:01:52Z

Staffer: Nomenclature updated

2014-03-13T17:10:21Z

Nomenclature updated

Admin: /* leaf -> (leaf) */

2013-09-09T19:29:20Z

‎leaf -> (leaf)

Admin at 19:22, 9 September 2013

2013-09-09T19:22:41Z

AHPAStaff: addition of hptlc info.--jd

2013-06-26T00:11:03Z

addition of hptlc info.--jd

New page

=Introduction=
[[Category:NoIntro]]

=Macroscopic Entries=
[[Category:NoMacroscopy]]

=Microscopic Entries=
[[Category:NoMicroscopy]]

=HPTLC Entries=
{{HPTLC | source=HPTLC Association
| companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
| companyURL=http://www.hptlc-association.org/
| mainimage=Artemisia annua-hptlc-association.png
| caption1=Sweet wormwood leaf, qing hao (leaf) HPTLC ID - NP reagent, UV 366 nm
| description=Sweet wormwood leaf, qing hao (leaf) (''Artemisia annua'')
|
| stationaryphase=Stationary phase, i.e. Silica gel 60, F254
| mobilephase=Ethyl acetate, water, acetic acid, formic acid 100:26:11:11 (v/v/v/v)
| prep=Sample: Mix 500 mg of powdered sample with 5 mL of methanol and sonicate for 10 minutes, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions.

Derivatization reagent: NP reagent, Preparation: 1 g of natural products reagent in 200 mL of ethyl acetate, Use: Heat plate for 3 min at 100°C, then dip (time 0, speed 5).
| detection=Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33%
| referencesamples=Reference: Dissolve 1 mg of chlorogenic acid in 1 mL of methanol, Dissolve 1 mg of caffeic acid in 1 mL of methanol, Optional: Dissolve 1 mg of rutin in 1 mL of methanol.
|
| lanes=Lanes, from left to right (Track, Volume, Sample):
# 5 µL Sweet wormwood fresh leaf 1 (extracted with water)
# 5 µL Sweet wormwood fresh leaf 1 (extracted with ethanol)
# 5 µL Sweet wormwood fresh leaf 1 (extracted with methanol)
# 5 µL Sweet wormwood fresh leaf 1 (extracted with hot water)
# 5 µL Sweet wormwood fresh leaf 2 (extracted with water)
# 5 µL Sweet wormwood fresh leaf 2 (extracted with ethanol)
# 5 µL Sweet wormwood fresh leaf 2 (extracted with methanol)
# 5 µL Sweet wormwood fresh leaf 2 (extracted with hot water)
# 5 µL Rutin, chlorogenic acid, caffeic acid (with increasing Rf)
# 5 µL Chinese wormwood fresh leaf (extracted with water)
# 5 µL Chinese wormwood fresh leaf (extracted with ethanol)
# 5 µL Chinese wormwood fresh leaf (extracted with methanol)
# 5 µL Chinese wormwood fresh leaf (extracted with hot water)
# '''5 µL Sweet wormwood dried leaf 3 (extracted with methanol)'''
# 5 µL Sweet wormwood dried leaf 3 (extracted with hot water)

| notes=''Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.''

System suitability test: Chlorogenic acid: green fluorescent zone at Rf ~ 0.40.
Caffeic acid: green fluorescent zone at Rf ~ 0.90.

Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows a green fluorescent zone at Rf ~ 0.40 corresponding to reference chlorogenic acid and below it a blue zone at Rf ~ 0.32 (yellow arrows). In the upper part of the chromatogram there is an intense green zone at Rf ~ 0.81 and a blue one just above it at Rf ~ 0.86. Below the solvent front there are two red zones. Similar but fainter zones are seen in the fresh sample (track 3).

Test for adulteration: The blue zones at Rf ~ 0.32 and Rf ~ 0.86 are missing (red arrows, Chinese wormwood leaf).

| }}

=Other Points of Interest=

[[Category:Blank2]]

@@ Line 14: / Line 14: @@
 =Botanical Voucher Specimen=
-{{Media2     |cat=Voucher
+{{Media4     | cat=Voucher
               | source=MOBOT, Tropicos.org
@@ Line 26: / Line 26: @@
               | companyURL=http://www.tropicos.org/Image/88841
               | reference=Tropicos.org. Missouri Botanical Garden. 06 Aug 2013 <http://www.tropicos.org/Image/100196967>, Tropicos.org. Missouri Botanical Garden. 06 Aug 2013 <http://www.tropicos.org/Image/88841>.
               | }}

@@ Line 1: / Line 1: @@
-{{DISPLAYTITLE: ''Artemisia annua '' (leaf) }}
+{{DISPLAYTITLE: ''Artemisia annua '' (leaf) }} {{askbox|herb=''Artemisia annua''}}
 =Nomenclature=
 {{nomenclature | binomial=Artemisia annua

@@ Line 14: / Line 14: @@
 =Botanical Voucher Specimen=
-{{Macroscopy | source=MOBOT, Tropicos.org
+{{Media2     |cat=Voucher
               | mainimage=Artemisia annua Tropicos 100196967.jpg
               | companyimage=TropicosLogo.gif
-              | companyURL=http://www.tropicos.org/Name/2701754
+              | companyURL=http://www.tropicos.org/Image/100196967
-              | caption1=''Artemisia annua'' - Tropicos.org (Hohenacker, Rudolph Friedrich - s.n.)
-| image2=Artemisia annua Tropicos 88841.jpg
+              | source2=MOBOT, Tropicos.org
-| caption2=''Artemisia annua'' - Tropicos.org (Yatskievych, George Alfred - 92-301)
+             | image2=Artemisia annua Tropicos 88841.jpg
-              | description=Sweet wormwood (leaf), qing hao (leaf) (''Artemisia annua'')
+             | companyimage2=TropicosLogo.gif
               | reference=Tropicos.org. Missouri Botanical Garden. 06 Aug 2013 <http://www.tropicos.org/Image/100196967>, Tropicos.org. Missouri Botanical Garden. 06 Aug 2013 <http://www.tropicos.org/Image/88841>.
               | }}

@@ Line 27: / Line 27: @@
 =Organoleptic Characteristics=
-=Macroscopic Descriptions=
+=Macroscopic Characteristics=
 =Microscopic Characteristics=

@@ Line 1: / Line 1: @@
 =Nomenclature=
 {{nomenclature | binomial=Artemisia annua
 |authority=L.
@@ Line 11: / Line 11: @@
 |notes= }}
-=Macroscopic Entries=
 {{Macroscopy | source=MOBOT, Tropicos.org
               | mainimage=Artemisia annua Tropicos 100196967.jpg
@@ Line 23: / Line 25: @@
               | }}
-=Microscopic Entries=
+=Organoleptic Characteristics=
-[[Category:NoMicroscopy]]
 {{HPTLC | source=HPTLC Association
               | companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
@@ Line 70: / Line 76: @@
               | }}
-=Other Points of Interest=
+<references />
+[[Category:Botanical]]
-[[Category:Blank2]]

@@ Line 10: / Line 10: @@
 | image2=Artemisia annua Tropicos 88841.jpg
 | caption2=''Artemisia annua'' - Tropicos.org (Yatskievych, George Alfred - 92-301)
-              | description=Sweet wormwood leaf, qing hao (leaf) (''Artemisia annua'')
+              | description=Sweet wormwood (leaf), qing hao (leaf) (''Artemisia annua'')
               | reference=Tropicos.org. Missouri Botanical Garden. 06 Aug 2013 <http://www.tropicos.org/Image/100196967>, Tropicos.org. Missouri Botanical Garden. 06 Aug 2013 <http://www.tropicos.org/Image/88841>.
               | }}
@@ Line 22: / Line 22: @@
               | companyURL=http://www.hptlc-association.org/
               | mainimage=Artemisia annua-hptlc-association.png
-              | caption1=Sweet wormwood leaf, qing hao (leaf) HPTLC ID - NP reagent, UV 366 nm
+              | caption1=Sweet wormwood (leaf), qing hao (leaf) HPTLC ID - NP reagent, UV 366 nm
-              | description=Sweet wormwood leaf, qing hao (leaf) (''Artemisia annua'')
+              | description=Sweet wormwood (leaf), qing hao (leaf) (''Artemisia annua'')
+              |
               | stationaryphase=Stationary phase, i.e. Silica gel 60, F254