Cannabis spp. (pistillate inflorescence and leaf) - Revision history

Admin: add micro images

2014-10-09T20:07:03Z

add micro images

Admin at 18:44, 9 October 2014

2014-10-09T18:44:25Z

Admin: description/title for HPTLC

2014-10-09T18:42:35Z

description/title for HPTLC

Admin at 18:41, 9 October 2014

2014-10-09T18:41:01Z

Admin: TLC

2014-10-09T18:39:44Z

TLC

Admin at 18:20, 9 October 2014

2014-10-09T18:20:32Z

Admin at 17:17, 9 October 2014

2014-10-09T17:17:34Z

Admin at 17:16, 9 October 2014

2014-10-09T17:16:44Z

Admin at 17:15, 9 October 2014

2014-10-09T17:15:45Z

Admin: add voucher specimen

2014-10-09T16:40:58Z

add voucher specimen

@@ Line 59: / Line 59: @@
 These are mainly associated with the flower structures (pistillate plants being particularly rich in these structures) but they can also be found on the underside of the leaves and occasionally on the stems of young plants.
 Some plants possess trichomes that may be confused with those present on Cannabis sativa and care should be taken in definitive identification. However, the combination of cystolithic hairs on the leaf upper surface and longer trichomes and sessile glands on the lower surface, which is unique to Cannabis sativa, enables positive identification of even fragmented material.”}}
 |}

@@ Line 111: / Line 111: @@
-{{HPTLC      | source=National Center for Natural Products Research, School of Pharmacy,
+{{HPTLC      | source=National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS 38677, USA
 University of Mississippi, University, MS 38677, USA
 | companyimage=OleMiss Logo.png
               | companyURL=http://pharmacy.olemiss.edu/ncnpr/
@@ Line 122: / Line 121: @@
               | prep=Test Sample Preparation: Prepare test sample as described under Reference Sample Preparations and apply 5uL.
 Derivatization reagent: Fast blue reagent– 5 mg/mL fast blue B salt in water.
-              | detection=
+              | detection=Development:Saturated chamber, developing distance 70 mm from lower edge of the plate; relative humidity 33%, temperature 25°.
 Development:Saturated chamber, developing distance 70 mm from lower edge of the plate; relative humidity 33%, temperature 25°.
 Detection: Heat plate at 70°C for 2 min, dip (time 5, speed 5) in Derivatization reagent, dry, and examine under white light.
@@ Line 143: / Line 141: @@
 # 2 uL Tetrahydrocannabivarin (THCV)
 # 5 uL Intermediate-type ''Cannabis'' decarboxylated
               | notes=''Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.''
@@ Line 151: / Line 148: @@
 The Test Sample Preparation chromatogram exhibits no zones or a minor zone at about one-third the chromatogram and at an Rf corresponding to cannabidiol in the chromatograms of the Reference Standard Solutions (cf.Intermediate-type ''Cannabis'' and Fiber-type ''Cannabis''). The Test Sample Preparation chromatogram exhibits minor zones in the upper-third of the chromatogram, above the zone corresponding to THCA in the chromatograms of the Reference Standard Solutions (''cf.'' Intermediate-type ''Cannabis'' and Fiber-type ''Cannabis'').
 | }}
-             | }}

@@ Line 111: / Line 111: @@
-{{HPTLC      | companyimage=OleMiss Logo.png
+{{HPTLC      | source=National Center for Natural Products Research, School of Pharmacy,
-             | companyURL=http://pharmacy.olemiss.edu/ncnpr/
 | source=National Center for Natural Products Research, School of Pharmacy,
 University of Mississippi, University, MS 38677, USA
               | mainimage=TLC - Cannabis spp - OleMiss.png
-              | caption1=''Cannabis'' spp. (rhizome) HPTLC ID - Anisaldehyde reagent, white RT
+              | caption1=''Cannabis'' spp. (rhizome) HPTLC ID - White RT
               | stationaryphase=Stationary Phase:TLC, Silica gel 60RP-18 F254, 150 um (Sorbent Technologies)
               | mobilephase=Mobile Phase:0.1% glacial acetic acid in a mixture of methanol and water (75:25)

@@ Line 111: / Line 111: @@
-{{HPTLC | source=National Center for Natural Products Research, School of Pharmacy,
+{{HPTLC      | companyimage=OleMiss Logo.png
 University of Mississippi, University, MS 38677, USA
               | mainimage=TLC - Cannabis spp - OleMiss.png
               | caption1=''Cannabis'' spp. (rhizome) HPTLC ID - Anisaldehyde reagent, white RT
@@ Line 128: / Line 128: @@
 Reference Sample Preparations:100 mg of powdered plant material in 10 mL of dichloromethane, sonicate for 1 h, filter, and evaporate the extract to dryness under nitrogen. Dissolve the residue in 10 mL of methanol.
+              |
               | lanes=Lanes, from left to right (Track, Volume, Sample):

← Older revision		Revision as of 18:39, 9 October 2014
Line 109:		Line 109:

	=High Performance Thin Layer Chromatographic Identification=		=High Performance Thin Layer Chromatographic Identification=
		+
		+
		+	{{HPTLC \| source=National Center for Natural Products Research, School of Pharmacy,
		+	University of Mississippi, University, MS 38677, USA
		+	\| companyimage=OleMiss Logo.png
		+	\| companyURL=http://pharmacy.olemiss.edu/ncnpr/
		+	\| mainimage=TLC - Cannabis spp - OleMiss.png
		+	\| caption1=''Cannabis'' spp. (rhizome) HPTLC ID - Anisaldehyde reagent, white RT
		+	\| stationaryphase=Stationary Phase:TLC, Silica gel 60RP-18 F254, 150 um (Sorbent Technologies)
		+	\| mobilephase=Mobile Phase:0.1% glacial acetic acid in a mixture of methanol and water (75:25)
		+	\| prep=Test Sample Preparation: Prepare test sample as described under Reference Sample Preparations and apply 5uL.
		+	Derivatization reagent: Fast blue reagent– 5 mg/mL fast blue B salt in water.
		+	\| detection=
		+	Development:Saturated chamber, developing distance 70 mm from lower edge of the plate; relative humidity 33%, temperature 25°.
		+
		+	Detection: Heat plate at 70°C for 2 min, dip (time 5, speed 5) in Derivatization reagent, dry, and examine under white light.
		+	\| referencesamples=Reference Standard Solutions: 1.0 mg/mL of each Δ9-Tetrahydrocannabinol, Δ9-tetrahydrocannabinolicacid, and cannabidiol in methanol.
		+
		+	Reference Sample Preparations:100 mg of powdered plant material in 10 mL of dichloromethane, sonicate for 1 h, filter, and evaporate the extract to dryness under nitrogen. Dissolve the residue in 10 mL of methanol.
		+
		+	\|
		+	\| lanes=Lanes, from left to right (Track, Volume, Sample):
		+	# 2 uL Δ9-Tetrahydrocannabinol (Δ9-THC or THC)
		+	# 2 uL Δ9-Tetrahydrocannabinolicacid (THCA)
		+	# 5 uL THC-type ''Cannabis''
		+	# 5 uL Intermediate-type ''Cannabis''
		+	# 5 uL Fiber-type ''Cannabis''
		+	# 2 uL Cannabidiolic acid (CBDA)
		+	# 2 uL Cannabidiol (CBD)
		+	# 2 uL Cannabichromene (CBC)
		+	# 2 uL Cannabigerol (CBG)
		+	# 2 uL Cannabinol (CBN)
		+	# 2 uL Tetrahydrocannabivarin (THCV)
		+	# 5 uL Intermediate-type ''Cannabis'' decarboxylated
		+
		+	\| notes=''Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.''
		+
		+	Identification:
		+	Compare Test Sample Preparation chromatogram with chromatograms of Reference Standard Solutions and Reference Sample Preparations. The Test Sample Preparation chromatogram is similar to that of the THC-type Reference Sample Preparation chromatogram. Additional weak zones may be present.
		+	The most intense zones in the chromatogram of the Test Sample Preparation are a purple zone in the lower-third section and a purple zone in the upper-third section of the chromatogram corresponding to THC and THCA, respectively,in the chromatograms of the Reference Standard Solutions and the THC-type Reference Sample Preparation.
		+
		+	The Test Sample Preparation chromatogram exhibits no zones or a minor zone at about one-third the chromatogram and at an Rf corresponding to cannabidiol in the chromatograms of the Reference Standard Solutions (cf.Intermediate-type ''Cannabis'' and Fiber-type ''Cannabis''). The Test Sample Preparation chromatogram exhibits minor zones in the upper-third of the chromatogram, above the zone corresponding to THCA in the chromatograms of the Reference Standard Solutions (''cf.'' Intermediate-type ''Cannabis'' and Fiber-type ''Cannabis'').
		+
		+	\| }}
		+

	=Supplementary Information=		=Supplementary Information=

@@ Line 38: / Line 38: @@
               | mainimage=Cannabis sativa - Köhler.jpg
               | reference=Köhler, Medizinal-Pflanzen in naturgetreuen Abbildungen und kurzerläuterndemTexte (1887)}}
 |}
 =Microscopic Characteristics=
 =Gas Chromatography Identification=
 =High Performance Thin Layer Chromatographic Identification=

@@ Line 25: / Line 25: @@
 {| border=1
+|
-{{Macroscopy| source=UNODC, Recommended methods for the identification and analysis of cannabis and cannabis products - ST/NAR/40
+{{Macroscopy| source=Recommended methods for the identification and analysis of cannabis and cannabis products, United Nations Office on Drugs and Crime, Vienna, 2009. (ST/NAR/40)
 |companyimage=UNODC Logo.png
 | companyURL=http://www.unodc.org/documents/scientific/ST-NAR-40-Ebook.pdf

@@ Line 14: / Line 14: @@
               | reference=Tropicos.org. Missouri Botanical Garden. 09 October 2014 http://www.tropicos.org/Image/100003863}}
 =Organoleptic Characteristics=
+{| border=1
 =Macroscopic Characteristics=
 =Microscopic Characteristics=

@@ Line 6: / Line 6: @@
 =Botanical Voucher Specimen=
+{{Media     | cat=Voucher
 =Organoleptic Characteristics=
@@ Line 12: / Line 18: @@
 =Microscopic Characteristics=
 =High Performance Thin Layer Chromatographic Identification=