Drynaria fortunei (rhizome) - Revision history

Admin: add askbox

2014-06-02T19:27:57Z

add askbox

Staffer at 22:53, 15 March 2014

2014-03-15T22:53:18Z

Staffer: Nomenclature updated

2014-03-13T22:58:00Z

Nomenclature updated

AHPAStaff: addition of hptlc info.--jd

2013-06-25T01:00:01Z

addition of hptlc info.--jd

New page

=Introduction=
[[Category:NoIntro]]

=Macroscopic Entries=
[[Category:NoMacroscopy]]

=Microscopic Entries=
[[Category:NoMicroscopy]]

=HPTLC Entries=

{{HPTLC | source=HPTLC Association
| companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
| companyURL=http://www.hptlc-association.org/
| mainimage=Drynaria fortunei-hptclc-association.png
| caption1=Drynaria rhizome, gu sui bu (rhizome) HPTLC ID - Aluminum chloride reagent, UV 366 nm
| description=Drynaria rhizome, gu sui bu (rhizome) (''Drynaria fortunei'')
|
| stationaryphase=Stationary phase, i.e. Silica gel 60, F254
| mobilephase=Ethyl acetate, acetic acid, formic acid, water 100:11:11:26 (v/v/v/v)
| prep=Sample: Mix 500 mg of powdered sample with 5 mL of methanol and sonicate for 10 minutes, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions.

Derivatization reagent: Aluminum chloride reagent R, Preparation: Dissolve 4 g of aluminum chloride in 200 mL of a 5% solution (v/v) of glacial acetic acid in methanol, Use: dip (time 0, speed 5).
| detection=Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33%
| referencesamples=Reference: Dissolve 1 mg of hyperoside in 1 mL of methanol; Dissolve 1 mg of naringin in 1 mL of methanol.
|
| lanes=Lanes, from left to right (Track, Volume, Sample):
# 5 µL Drynaria rhizome 1
# '''10 µL Drynaria rhizome 1'''
# 15 µL Drynaria rhizome 1
# 5 µL Hyperoside
# 5 µL Naringin
# 10 µL Drynaria rhizome 2
# 10 µL Drynaria rhizome 3
# Blank
# 10 µL ''Humata tyermanni'' Moore rhizome
# 10 µL ''Drynaria bonii'' rhizome
# 10 µL ''Drynaria rigidula'' rhizome
# 10 µL ''Drynaria sparsisora'' rhizome

| notes=''Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.''

System suitability test: Hyperoside: yellow fluorescent zone at Rf ~ 0.50; Naringin: blue white fluorescent zone at Rf ~ 0.42.

Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows an intense greenish blue white zone at Rf ~ 0.42 corresponding to reference substance naringin. Right below this zone there is a less intense greenish blue white zone at Rf ~ 0.38.

Test for other species: No intense zone is seen between the application position and Rf ~ 0.30 (Humata tyermanni Moore, Drynaria bonii, Drynaria rigidula, Drynaria sparsisora) and no zone is seen at the position of reference substance hyperoside (Humata tyermanni Moore) (pink arrows).

| }}

=Other Points of Interest=

[[Category:Blank2]]

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-{{DISPLAYTITLE:''Drynaria fortunei'' (rhizome) }}
+{{DISPLAYTITLE:''Drynaria fortunei'' (rhizome) }} {{askbox|herb=''Drynaria fortunei''}}
-−
 =Nomenclature=
 {{nomenclature | binomial=Drynaria fortunei
 |authority=(Kunze ex Mett.) J. Sm.

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 =Nomenclature=
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 |notes= }}
-=Macroscopic Entries=
+=Botanical Voucher Specimen=
-[[Category:NoMacroscopy]]
+=Organoleptic Characteristics=
-=Microscopic Entries=
+=Macroscopic Characteristics=
-[[Category:NoMicroscopy]]
+=Microscopic Characteristics=
-=HPTLC Entries=
+=High Performance Thin Layer Chromatographic Identification=
 {{HPTLC | source=HPTLC Association
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               | }}
-=Other Points of Interest=
+=Supplementary Information=
+=Sources=
-[[Category:Blank2]]
+<references />