Eclipta prostrata (aerial parts) - Revision history

Staffer: Botanical Voucher Kew added

2014-08-19T00:32:53Z

Botanical Voucher Kew added

Admin: add askbox

2014-06-02T19:26:08Z

add askbox

Staffer: Voucher added

2014-03-22T04:09:52Z

Voucher added

Staffer: Various title corrections

2014-03-15T23:03:52Z

Various title corrections

Staffer: Nomenclature updated

2014-03-13T23:04:09Z

Nomenclature updated

AHPAStaff: addition of hptlc info.--jd

2013-06-25T01:02:27Z

addition of hptlc info.--jd

New page

=Introduction=
[[Category:NoIntro]]

=Macroscopic Entries=
[[Category:NoMacroscopy]]

=Microscopic Entries=
[[Category:NoMicroscopy]]

=HPTLC Entries=
{{HPTLC | source=HPTLC Association
| companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
| companyURL=http://www.hptlc-association.org/
| mainimage=Eclipta prostrata-hptclc-association.png
| caption1=False daisy herb, mo han lian (aerial parts) HPTLC ID - NP and PEG reagent, UV 366 nm
| description=False daisy herb, mo han lian (aerial parts) (''Eclipta prostrata'')
|
| stationaryphase=Stationary phase, i.e. Silica gel 60, F254
| mobilephase=Toluene, acetone, formic acid 11:6:1 (v/v/v)
| prep=Sample: Mix 500 mg of powdered sample with 5 mL of methanol and sonicate for 10 minutes at 60°C, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions.

Derivatization reagent: 1.) NP reagent, Preparation: 1 g of natural products reagent in 200 mL of ethyl acetate; 2.) PEG reagent, Preparation: 10 g of polyethylene glycol 400 in 200 mL of dichloromethane, Use: Heat plate 3 min at 100°C, dip (time 0, speed 5) in NP reagent, dry and dip (time 0, speed 5) in PEG reagent
| detection=Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33%
| referencesamples=Reference: Dissolve 1 mg of quercetin and 1 mg of rosmarinic acid in 1 mL of methanol.
Optional: dissolve 1 mg of wedelolactone in 1 mL of methanol.
|
| lanes=Lanes, from left to right (Track, Volume, Sample):
# 2 µL False daisy herb 1
# '''5 µL False daisy herb 1'''
# 7 µL False daisy herb 1
# 2 µL False daisy herb 2
# '''5 µL False daisy herb 2'''
# 7 µL False daisy herb 2
# 3 µL Quercetin
# 3 µL Rosmarinic acid
# 3 µL Wedelolactone
# '''5 µL False daisy herb 3'''
# '''5 µL False daisy herb 4'''
# '''5 µL False daisy herb 5'''
# '''5 µL False daisy herb 6'''
# 5 µL False daisy herb (questionable)
# 5 µL Great St. John’s wort herb

| notes=''Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.''

System suitability test: Quercetin: orange fluorescent zone at Rf ~ 0.48; Rosmarinic acid: blue fluorescent zone at Rf ~ 0.27.

Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows several blue white or greenish white zones just above the start position. There is a faint blue white zone below the position of reference quercetin and a blue white zone (wedelolactone) at the position of quercetin. Above it there is a pale blue zone. Above this zone, just below the solvent front, there is a pattern of three diffuse zones, a greenish, a blue violet and a red zone.

Test for adulteration: No blue white zones are seen at the position of quercetin and just below it (between Rf ~ 0.37-0.47) (purple arrow, Great John’s wort herb).

| }}

=Other Points of Interest=

[[Category:Blank2]]

@@ Line 14: / Line 14: @@
 =Botanical Voucher Specimen=
-{{Media      |cat=Voucher
+{{Media4      |cat=Voucher
               | source=MOBOT, Tropicos.org
@@ Line 21: / Line 21: @@
               | companyURL=http://www.tropicos.org/Image/100011452
               | reference=Tropicos.org. Missouri Botanical Garden. 21 Mar 2014 <http://www.tropicos.org/Image/100011452>
               | }}

← Older revision		Revision as of 19:26, 2 June 2014
Line 1:		Line 1:
−	{{DISPLAYTITLE:''Eclipta prostrata'' (aerial parts) }}	+	{{DISPLAYTITLE:''Eclipta prostrata'' (aerial parts) }} {{askbox\|herb=''Eclipta prostrata''}}
	=Nomenclature=		=Nomenclature=

← Older revision		Revision as of 04:09, 22 March 2014
Line 13:		Line 13:

	=Botanical Voucher Specimen=		=Botanical Voucher Specimen=
		+
		+	{{Media \|cat=Voucher
		+
		+	\| source=MOBOT, Tropicos.org
		+	\| mainimage=Eclipta prostrata Tropicos 100011452.jpg
		+	\| companyimage=TropicosLogo.gif
		+	\| companyURL=http://www.tropicos.org/Image/100011452
		+	\| reference=Tropicos.org. Missouri Botanical Garden. 21 Mar 2014 <http://www.tropicos.org/Image/100011452>
		+
		+	\| }}
		+
		+
	=Organoleptic Characteristics=		=Organoleptic Characteristics=

@@ Line 1: / Line 1: @@
 =Nomenclature=
@@ Line 11: / Line 12: @@
 |notes= }}
-=Macroscopic Entries=
+=Botanical Voucher Specimen=
-[[Category:NoMacroscopy]]
+=Organoleptic Characteristics=
-=Microscopic Entries=
+=Macroscopic Characteristics=
-[[Category:NoMicroscopy]]
+=Microscopic Characteristics=
 {{HPTLC | source=HPTLC Association
               | companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
@@ Line 62: / Line 64: @@
-=Other Points of Interest=
+=Supplementary Information=
+=Sources=
-[[Category:Blank2]]
+<references />