Equisetum palustre (leaf) - Revision history

Staffer: Botanical Voucher Kew added

2014-08-19T01:37:35Z

Botanical Voucher Kew added

Admin: add askbox

2014-06-02T18:10:05Z

add askbox

Staffer at 23:17, 15 March 2014

2014-03-15T23:17:35Z

Staffer: Various title corrections, Source additions

2014-03-15T23:17:00Z

Various title corrections, Source additions

AHPAStaff: addition of hptlc info.--jd

2013-06-25T01:16:58Z

addition of hptlc info.--jd

New page

=Introduction=
[[Category:NoIntro]]

=Macroscopic Entries=
[[Category:NoMacroscopy]]

=Microscopic Entries=
[[Category:NoMicroscopy]]

=HPTLC Entries=
{{HPTLC | source=HPTLC Association
| companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
| companyURL=http://www.hptlc-association.org/
| mainimage=Equisetum palustre-hptclc-association.png
| caption1=Marsh horsetail herb (leaf) HPTLC ID - NP and PEG reagent, UV 366 nm
| description=Marsh horsetail herb (leaf) (''Equisetum palustre'')
|
| stationaryphase=Stationary phase, i.e. Silica gel 60, F254
| mobilephase=Ethyl acetate, water, acetic acid, formic acid 134:36:15:15 (v/v/v/v)
| prep=Sample: Mix 1 g of powdered sample with 10 mL of methanol and sonicate for 10 minutes, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions.

Derivatization reagent: NP reagent, Preparation: 1 g of natural products reagent in 200 mL of ethyl acetate; 2.) PEG reagent, Preparation: 10 g of polyethylene glycol 400 in 200 mL of methylene chloride, Use: Heat plate for 3 min at 100°C, dip (time 0, speed 5) in NP reagent, dry and dip (time 0, speed 5) in PEG reagent.
| detection=Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33%
| referencesamples=Reference: Individually dissolve 1 mg of caffeic acid, 1 mg of rutin, and 1 mg of hyperoside each in 1 mL of methanol.
|
| lanes=Lanes, from left to right (Track, Volume, Sample):
# 2 µL Marsh horsetail herb 1
# '''5 µL Marsh horsetail herb 1'''
# 8 µL Marsh horsetail herb 1
# 1 µL Rutin, hyperoside, caffeic acid (with increasing Rf)
# 2 µL Common horsetail herb 1
# 5 µL Common horsetail herb 1
# 8 µL Common horsetail herb 1
# 5 µL Common horsetail herb 2
# 5 µL Common horsetail herb 3
# 5 µL Common horsetail herb 4
# 5 µL Common horsetail herb 5
# 5 µL Common horsetail herb 6 (adulterated with Marsh horsetail herb)
# 5 µL Common horsetail herb 2 (adulterated with Marsh horsetail herb)
# 5 µL Common horsetail herb 3 (adulterated with Marsh horsetail herb)

| notes=''Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.''

System suitability test: Rutin: orange fluorescent zone at Rf ~ 0.34; Hyperoside: orange fluorescent zone at Rf ~ 0.50; Caffeic acid: light blue fluorescent zone at Rf ~ 0.88.

Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solutions shows four green zones between the application position and the zone due to reference substance rutin (green arrows). Right above the zone due to rutin there is a faint blue zone. Just below the solvent front there are two red zones due to chlorophylls.

Test for adulteration: There is no yellow zone at or right above the position of hyperoside (pink arrow); between hyperoside and caffeic acid there are not two intense blue zones (white arrow, Common horsetail herb).

| }}

=Other Points of Interest=

[[Category:Blank2]]

← Older revision		Revision as of 01:37, 19 August 2014
Line 3:		Line 3:

	=Botanical Voucher Specimen=		=Botanical Voucher Specimen=
		+	{{Media \|cat=Voucher
		+	\| companyimage=Kewlogo.gif
		+	\| companyURL=http://specimens.kew.org/herbarium/K001057681
		+	\| mainimage=Equisetum_palustre_Kew_imageBarcode=K001057681_650549.jpg
		+	\| source=Royal Botanic Gardens, Kew.
		+	}}
		+
		+
	=Organoleptic Characteristics=		=Organoleptic Characteristics=

← Older revision		Revision as of 18:10, 2 June 2014
Line 1:		Line 1:
−	{{DISPLAYTITLE:''Equisetum palustre'' (leaf) }}	+	{{DISPLAYTITLE:''Equisetum palustre'' (leaf) }} {{askbox\|herb=''Equisetum palustre''}}
	=Nomenclature=		=Nomenclature=

@@ Line 1: / Line 1: @@
 {{DISPLAYTITLE:''Equisetum palustre'' (leaf) }}
+=Nomenclature=
 =Botanical Voucher Specimen=

@@ Line 1: / Line 1: @@
-=Introduction=
+{{DISPLAYTITLE:''Equisetum palustre'' (leaf) }}
-[[Category:NoIntro]]
-=Microscopic Entries=
+=Botanical Voucher Specimen=
-[[Category:NoMicroscopy]]
+=Organoleptic Characteristics=
 {{HPTLC | source=HPTLC Association
               | companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
@@ Line 50: / Line 51: @@
               | }}
-=Other Points of Interest=
+=Supplementary Information=
-−
-[[Category:Blank2]]
+=Sources=