Harpagophytum spp. (root) - Revision history

Admin: add PlantaPhile references

2015-05-05T21:13:29Z

add PlantaPhile references

Staffer: Botanical Voucher added

2014-08-20T01:57:20Z

Botanical Voucher added

Admin: add askbox

2014-06-02T20:11:10Z

add askbox

Staffer at 23:47, 15 March 2014

2014-03-15T23:47:56Z

Admin: new layout

2014-02-14T19:30:29Z

new layout

AHPAStaff: addition of hptlc info.--jd

2013-06-25T00:23:58Z

addition of hptlc info.--jd

New page

=Introduction=
[[Category:NoIntro]]

=Macroscopic Entries=
[[Category:NoMacroscopy]]

=Microscopic Entries=
[[Category:NoMicroscopy]]

=HPTLC Entries=
{{HPTLC | source=HPTLC Association
| companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
| companyURL=http://www.hptlc-association.org/
| mainimage=Harpagophytum spp-hptlc-association.png
| caption1=Devil’s claw (root) HPTLC ID - Anisaldehyde reagent, white RT
| description=Devil’s claw (root) (''Harpagophytum procumbens'' and/or ''Harpagophytum zeyheri'')
|
| stationaryphase=Stationary phase, i.e. Silica gel 60, F254
| mobilephase=Ethyl acetate, methanol, water 77:15:8 (v/v/v)
| prep=Sample: Heat 1.0 g of the powdered drug with 10 mL of methanol, sonicate on a water-bath at 60°C for 10 min. Centrifuge and reduce the filtered solution to 2 mL under reduced pressure at a temperature not exceeding 40°C.

Derivatization reagent: Anisaldehyde reagent, Preparation: 170 mL of ice-cooled methanol are mixed with 20 mL of glacial acetic acid, 10 mL of concentrated sulfuric acid and 1 mL of anisaldehyde, Use: Dip (time 0, speed 5), heat at 100°C for 3 min.
| detection=Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33%
| referencesamples=Reference: Heat 1.0 g of the powdered drug with 10 mL of methanol, sonicate on a water-bath at 60°C for 10 min. Centrifuge and reduce the filtered solution to 2 mL under reduced pressure at a temperature not exceeding 40°C.
|
| lanes=Lanes, from left to right (Track, Volume, Sample):
# (4) 1 µL Devil’s claw root 1
# (5) '''2 µL Devil’s claw root 1'''
# (6) 4 µL Devil’s claw root 1
# (7) 2 µL Devil’s claw root 2
# (8) 2 µL Devil’s claw root 3 (old sample)
# (9) 2 µL Devil’s claw root 4 (old sample)
# (10) 2 µL Devil’s claw root 5 (old sample)
# (11) 5 µL Harpagoside
# (12) 5 µL Fructose

| notes=''Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.''

System suitability test: Harpagoside: brown zone at Rf ~ 0.44; Fructose: greenish zone at Rf ~ 0.14.

Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows a brown zone (Rf ~ 0.06) below the zone due to reference substance fructose. At the position of fructose there may be a greenish zone (see track 9). Above fructose there is an intense brown zone at Rf ~ 0.18, just above it a blue zone at Rf ~ 0.22 and at Rf ~ 0.32 a weak brown zone. An intense brown zone corresponding to harpagoside is seen at Rf ~ 0.44.

| }}

=Other Points of Interest=

[[Category:Blank2]]

@@ Line 48: / Line 48: @@
 =Macroscopic Characteristics=
+{{ Media    | cat=Macroscopy
 =Microscopic Characteristics=

← Older revision		Revision as of 01:57, 20 August 2014
Line 3:		Line 3:

	=Botanical Voucher Specimen=		=Botanical Voucher Specimen=
		+
		+	{{media4 \|cat=voucher
		+
		+
		+	\| source=Royal Botanic Gardens, Kew.
		+	\| companyimage=Kewlogo.gif
		+	\| companyURL=http://specimens.kew.org/herbarium/K000057627
		+	\| mainimage=Harpagophytum_procumbens_subsp._procumbens_Kew_imageBarcode%3DK000057627_180053.jpg
		+	\| caption1=''Harpagophytum procumbens'' subsp. ''procumbens''
		+
		+	\| source2=Royal Botanic Gardens, Kew.
		+	\| companyimage2=Kewlogo.gif
		+	\| companyURL2=http://specimens.kew.org/herbarium/K000901661
		+	\| image2=Fraxinus_excelsior_Kew_imageBarcode%3DK000901661_517288.jpg
		+	\| caption2=''Harpagophytum procumbens'' subsp. ''procumbens''
		+
		+	\| source3=Royal Botanic Gardens, Kew.
		+	\| companyimage3=Kewlogo.gif
		+	\| companyURL3=http://specimens.kew.org/herbarium/K000058171
		+	\| image3=Harpagophytum_zeyheri_subsp._schijffii_Kew_imageBarcode%3DK000058171_197600.jpg
		+	\| caption3=''Harpagophytum zeyheri'' subsp. ''schijffii''
		+
		+	\| companyimage4=Kewlogo.gif
		+	\| source4=Royal Botanic Gardens, Kew.
		+	\| companyURL4=http://specimens.kew.org/herbarium/K000057642
		+	\| image4=Harpagophytum_zeyheri_subsp._sublobatum_Kew_imageBarcode%3DK000057642_180054.jpg
		+	\| caption4=''Harpagophytum zeyheri'' subsp. ''sublobatum''
		+
		+	\| }}
		+
		+	{{Media \| cat=Voucher
		+
		+	\| source=Royal Botanic Gardens, Kew.
		+	\| companyimage=Kewlogo.gif
		+	\| companyURL=http://specimens.kew.org/herbarium/K000058191
		+	\| mainimage=Harpagophytum_zeyheri_subsp._zeyheri_Kew_imageBarcode%3DK000058191_197601.jpg
		+	\| caption1=''Harpagophytum zeyheri'' subsp. ''zeyheri''
		+	\| }}
		+
		+
		+

	=Organoleptic Characteristics=		=Organoleptic Characteristics=

← Older revision		Revision as of 20:11, 2 June 2014
Line 1:		Line 1:
−	{{DISPLAYTITLE:''Harpagophytum'' spp. (root)}}	+	{{DISPLAYTITLE:''Harpagophytum'' spp. (root)}} {{askbox\|herb=''Harpagophytum'' spp.}}
	=Nomenclature=		=Nomenclature=

@@ Line 6: / Line 6: @@
 =Organoleptic Characteristics=
-=Macroscopic Descriptions=
+=Macroscopic Characteristics=
 =Microscopic Characteristics=

@@ Line 1: / Line 1: @@
-=Introduction=
+{{DISPLAYTITLE:''Harpagophytum'' spp. (root)}}
-[[Category:NoIntro]]
+=Nomenclature=
-=Macroscopic Entries=
+=Botanical Voucher Specimen=
-[[Category:NoMacroscopy]]
-=Microscopic Entries=
+=Organoleptic Characteristics=
-[[Category:NoMicroscopy]]
-=HPTLC Entries=
+=Macroscopic Descriptions=
 {{HPTLC | source=HPTLC Association
               | companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
@@ Line 15: / Line 17: @@
               | caption1=Devil’s claw (root) HPTLC ID - Anisaldehyde reagent, white RT
               | description=Devil’s claw (root) (''Harpagophytum procumbens'' and/or ''Harpagophytum zeyheri'')
               | stationaryphase=Stationary phase, i.e. Silica gel 60, F254
               | mobilephase=Ethyl acetate, methanol, water 77:15:8 (v/v/v)
@@ Line 43: / Line 44: @@
               | }}
-=Other Points of Interest=
+<references />
+[[Category:Botanical]]
-[[Category:Blank2]]