Levisticum officinale (root) - Revision history

Admin: add Kew voucher specimen reference

2015-05-26T14:48:49Z

add Kew voucher specimen reference

Admin: add PlantaPhile references

2015-05-05T20:50:54Z

add PlantaPhile references

Admin: add askbox

2014-06-02T20:34:31Z

add askbox

Staffer: Voucher added

2014-03-25T07:02:50Z

Voucher added

Staffer: Various title corrections, Source additions

2014-03-16T01:19:26Z

Various title corrections, Source additions

Staffer: Nomenclature updated

2014-03-14T18:17:21Z

Nomenclature updated

AHPAStaff: addition of hptlc info.--jd

2013-06-25T01:16:20Z

addition of hptlc info.--jd

New page

=Introduction=
[[Category:NoIntro]]

=Macroscopic Entries=
[[Category:NoMacroscopy]]

=Microscopic Entries=
[[Category:NoMicroscopy]]

=HPTLC Entries=
{{HPTLC | source=HPTLC Association
| companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
| companyURL=http://www.hptlc-association.org/
| mainimage=Levisticum officinale-UV 254 nm-hptclc-association.png
| caption1=Lovage (root) HPTLC ID - UV 254 nm
| description=Lovage (root) (''Levisticum officinale'')
| image2=Levisticum officinale-UV 366 nm-hptclc-association.png
| caption2=Lovage (root) HPTLC ID - UV 366 nm
| image3=Levisticum officinale-Sulfuric acid, WRT-hptclc-association.png
| caption3=Lovage (root) HPTLC ID - Sulfuric acid, WRT
|
| stationaryphase=Stationary phase, i.e. Silica gel 60, F254
| mobilephase=Toluene, ethyl acetate, glacial acetic acid 90:10:1 (v/v/v)
| prep=Sample: Mix 1.0 g of powdered sample with 4 mL of heptane and sonicate for 5 minutes, then centrifuge and filter the solutions and use the filtrates as test solutions.

Derivatization reagent: Sulfuric acid reagent, Preparation: 20 mL sulfuric acid with 180 mL methanol, Use: Dip for 1 s, heat at 100°C for 5 min.
| detection=Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33%
| referencesamples=Reference: Dissolve 1 mg each of osthole and imperatorin in 10 mL of methanol. Optional: Dissolve 1 mg of Z-ligustilide in 10 mL of methanol.
|
| lanes=Lanes, from left to right (Track, Volume, Sample):
# 4 µL Imperatorin, z-Ligustilide (incr. Rf)
# 4 µL Osthole
# 4 µL Angelica root 1 (these samples do not comply with Ph.Eur specifications)
# 4 µL Angelica root 2
# 4 µL Angelica root 3
# 4 µL Chinese Angelica root
# 4 µL Dahurian Angelica root
# 4 µL Doubleteeth pubescent Angelica root 1
# 4 µL Doubleteeth pubescent Angelica root 2
# 4 µL Lovage root 1 (these samples do not comply with Ph.Eur specifications)
# '''4 µL Lovage root 2'''
# '''4 µL Lovage root 3'''
# 4 µL Chinese lovage root (''Ligusticum sinensis'')
# 4 µL Chinese lovage root 1 (''Ligusticum jeholense'')
# 4 µL Chinese lovage root 2 (''Ligusticum jeholense'')
# 4 µL Chinese lovage root (''Ligusticum chuanxiong'')

| notes=''Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.''

System suitability test: Imperatorin: greenish fluorescent zone at Rf ~ 0.31 (UV 366 nm); Osthole: blue fluorescent zone at Rf ~ 0.36 (UV 366 nm).

Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. Under UV 254 nm the chromatogram of the test solution shows an intense blue fluorescent zone at Rf ~ 0.57 and a weak quenching zone corresponding to reference osthole (Rf ~ 0.36) (red arrows). Under UV 366 nm the chromatogram of the test solution shows an intense blue white fluorescent zone at Rf ~ 0.57 and a weak whitish fluorescent zone with similar Rf-value as reference imperatorin (yellow arrows). After derivatization under white light there are two prominent purple zones below the solvent front. Below reference imperatorin there are two distinct purple zones and below them is an intense greenish zone.

Test for adulteration: Under UV 254 nm no zone is seen at or directly below the position of imperatorin. Under UV 366 nm there is no zone at the position of osthole. After derivatization under white light there are no zones at the position of imperatorin and osthole and just below the position of imperatorin there is no prominent diffuse purple zone (red arrow) (Angelica root, Chinese Angelica root, Doubleteeth pubescent root, Dahurian Angelica root, Chinese Lovage root).

| }}

=Other Points of Interest=

[[Category:Blank2]]

@@ Line 14: / Line 14: @@
 =Botanical Voucher Specimen=
-{{Media      |cat=Voucher
+{{Media2      |cat=Voucher
               | source=MOBOT, Tropicos.org
@@ Line 22: / Line 22: @@
               | reference=Tropicos.org. Missouri Botanical Garden. 25 Mar 2014 <http://www.tropicos.org/Image/88150>
               | }}

← Older revision		Revision as of 20:50, 5 May 2015
Line 27:		Line 27:

	=Macroscopic Characteristics=		=Macroscopic Characteristics=
		+	{{ Media2 \| cat=Macroscopy
		+	\| source=PlantaPhile
		+	\| mainimage=PlantaPhile - 129.jpg
		+	\| companyimage=PlantaPhile logo.jpg
		+	\| companyURL=http://plantaphile.com/
		+
		+	\| source2=PlantaPhile
		+	\| image2=PlantaPhile - 2656.jpg
		+	\| companyimage2=PlantaPhile logo.jpg
		+	\| companyURL2=http://plantaphile.com/
		+	\| }}
	=Microscopic Characteristics=		=Microscopic Characteristics=

← Older revision		Revision as of 20:34, 2 June 2014
Line 1:		Line 1:
−	{{DISPLAYTITLE:''Levisticum officinale'' (root) }}	+	{{DISPLAYTITLE:''Levisticum officinale'' (root) }} {{askbox\|herb=''Levisticum officinale''}}
	=Nomenclature=		=Nomenclature=

← Older revision		Revision as of 07:02, 25 March 2014
Line 13:		Line 13:

	=Botanical Voucher Specimen=		=Botanical Voucher Specimen=
		+
		+	{{Media \|cat=Voucher
		+
		+	\| source=MOBOT, Tropicos.org
		+	\| mainimage=Levisticum_officinale_Tropicos_88150.jpg
		+	\| companyimage=TropicosLogo.gif
		+	\| companyURL=http://www.tropicos.org/Image/88150
		+	\| reference=Tropicos.org. Missouri Botanical Garden. 25 Mar 2014 <http://www.tropicos.org/Image/88150>
		+
		+	\| }}
		+
	=Organoleptic Characteristics=		=Organoleptic Characteristics=

@@ Line 1: / Line 1: @@
 =Nomenclature=
@@ Line 11: / Line 12: @@
 |notes= }}
-=Macroscopic Entries=
+=Macroscopic Characteristics=
-[[Category:NoMacroscopy]]
+=Microscopic Characteristics=
-=Microscopic Entries=
+=High Performance Thin Layer Chromatographic Identification=
-[[Category:NoMicroscopy]]
-−
-=HPTLC Entries=
 {{HPTLC | source=HPTLC Association
               | companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
@@ Line 67: / Line 67: @@
               | }}
-=Other Points of Interest=
+=Supplementary Information=
+=Sources=
-[[Category:Blank2]]
+<references />