Melissa officinalis (leaf) - Revision history

Admin: add Culbreth ref for organoleptic, macro

2015-07-07T20:49:33Z

add Culbreth ref for organoleptic, macro

Admin: add macro and organoleptic characteristics

2015-06-16T19:46:31Z

add macro and organoleptic characteristics

Admin: add Kew voucher specimen reference

2015-05-26T15:16:38Z

add Kew voucher specimen reference

Admin: add PlantaPhile references

2015-05-05T20:44:03Z

add PlantaPhile references

Admin: add askbox

2014-06-02T20:37:35Z

add askbox

Staffer at 06:03, 26 March 2014

2014-03-26T06:03:04Z

Staffer: Voucher added

2014-03-26T06:01:34Z

Voucher added

Staffer: Various title corrections, Source additions

2014-03-16T01:41:40Z

Various title corrections, Source additions

Staffer: Nomenclature updated

2014-03-14T18:56:56Z

Nomenclature updated

AHPAStaff: addition of hptlc info.--jd

2013-06-25T23:44:04Z

addition of hptlc info.--jd

New page

=Introduction=
[[Category:NoIntro]]

=Macroscopic Entries=
[[Category:NoMacroscopy]]

=Microscopic Entries=
[[Category:NoMicroscopy]]

=HPTLC Entries=
{{HPTLC | source=HPTLC Association
| companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
| companyURL=http://www.hptlc-association.org/
| mainimage=Melissa officinalis-hptclc-association.png
| caption1=Melissa leaf dry extract (leaf) HPTLC ID - NP reagent, UV 366 nm
| description=Melissa leaf dry extract (leaf) (''Melissa officinalis'')
|
| stationaryphase=Stationary phase, i.e. Silica gel 60, F254
| mobilephase=Formic acid, water, ethyl acetate 1:1:15 (v/v/v)
| prep=Sample: Mix 0.2 g of extract with 5 mL of methanol and sonicate for 5 minutes, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions. Optional: Mix 0.5 g of dried Melissa leaf with 5 mL methanol and proceed as above.

Derivatization reagent: NP reagent, Preparation: 1 g of natural products reagent in 200 mL of ethyl acetate; 2.) PEG reagent, Preparation: 10 g of polyethylene glycol 400 in 200 mL of methylene chloride, Use: Heat plate for 3 min at 100°C, dip (time 0, speed 5) in NP reagent, dry and dip (time 0, speed 5) in PEG reagent, dry in air.
| detection=Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33%
| referencesamples=Reference: Dissolve 1 mg of hyperoside, 1 mg of rutin and 5 mg of rosmarinic acid individually in 10 mL of methanol.
|
| lanes=Lanes, from left to right (Track, Volume, Sample):
# 2 µL Rutin, hyperoside, rosmarinic acid (with increasing Rf)
# '''2 µL Melissa leaf dry extract 1'''
# '''2 µL Melissa leaf dry extract 2'''
# 2 µL Melissa leaf dry extract 3
# 2 µL Melissa leaf dry extract 4
# 2 µL Melissa leaf dry extract 5 (adulterated with Peppermint leaf)
# 2 µL Melissa leaf dry extract 6
# 2 µL Peppermint leaf dry extract
# 2 µL Melissa leaf 1
# 2 µL Melissa leaf 2
# 2 µL Melissa leaf 3
# 2 µL Melissa leaf 4
# 2 µL Melissa leaf 5
# 2 µL Peppermint leaf

| notes=''Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.''

System suitability test: Rutin: yellow fluorescent zone Rf ~ 0.09; Hyperoside: yellow fluorescent zone at Rf ~ 0.25; Rosmarinic acid: light blue fluorescent zone at Rf ~ 0.74.

Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows a light blue fluorescent zone just below the position of reference hyperoside. At the position of rosmarinic acid there is an intense light blue fluorescent zone. Between this zone and reference hyperoside there are two bluoe fluorescent zones (green arrows).

Test for other species: No yellow zones are seen below the position of reference hyperoside (pink arrow, Peppermint dry extract).

| }}

=Other Points of Interest=

[[Category:Blank2]]

@@ Line 31: / Line 31: @@
 {| border=1
+|
 {{Organolepsy | source=American Medicinal Plants of Commercial Importance (1930)
 | description=[''Melissa officinalis''] is lemon scented.
@@ Line 38: / Line 40: @@
 {| border=1
+|
 {{Macroscopy | source=American Medicinal Plants of Commercial Importance (1930)
 | description=This plant is 1 to 2 1/2 feet high, is covered with fine hair, and has a rather stout, erect, or much-branched stem.  The round-toothed, egg-shaped or heart-shaped leaves are from 1 to 2 1/2 inches long and arranged opposite one another on the stem.  From June to August the white or cream-colored tube shaped flowers up to two-thirds of an inch long appear, several to a cluster, in the axils of the leaves.

@@ Line 29: / Line 29: @@
 =Organoleptic Characteristics=
+{| border=1
 =Macroscopic Characteristics=
 {{ Media2    | cat=Macroscopy
               | source=PlantaPhile
@@ Line 42: / Line 52: @@
               | companyURL2=http://plantaphile.com/
               | }}
 =Microscopic Characteristics=

@@ Line 13: / Line 13: @@
 =Botanical Voucher Specimen=
-{{Media      |cat=Voucher
+{{Media2      |cat=Voucher
               | source=MOBOT, Tropicos.org
@@ Line 20: / Line 20: @@
               | companyURL=http://www.tropicos.org/Image/100007389
               | reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/100007389>
               | }}

← Older revision		Revision as of 20:44, 5 May 2015
Line 26:		Line 26:

	=Macroscopic Characteristics=		=Macroscopic Characteristics=
		+	{{ Media2 \| cat=Macroscopy
		+	\| source=PlantaPhile
		+	\| mainimage=PlantaPhile - 174.jpg
		+	\| companyimage=PlantaPhile logo.jpg
		+	\| companyURL=http://plantaphile.com/
		+
		+	\| source2=PlantaPhile
		+	\| image2=PlantaPhile - 1914.jpg
		+	\| companyimage2=PlantaPhile logo.jpg
		+	\| companyURL2=http://plantaphile.com/
		+	\| }}
	=Microscopic Characteristics=		=Microscopic Characteristics=

@@ Line 1: / Line 1: @@
-{{DISPLAYTITLE:''Melissa officinalis'' (leaf) }}
+{{DISPLAYTITLE:''Melissa officinalis'' (leaf) }} {{askbox|herb=''Melissa officinalis''}}
 =Nomenclature=
 {{nomenclature | binomial=Melissa officinalis
 |authority=L.

← Older revision		Revision as of 06:01, 26 March 2014
Line 13:		Line 13:

	=Botanical Voucher Specimen=		=Botanical Voucher Specimen=
		+
		+	{{Media \|cat=Voucher
		+
		+	\| source=MOBOT, Tropicos.org
		+	\| mainimage=Melissa_officinalis_Tropicos_100007389_(S).jpg
		+	\| companyimage=TropicosLogo.gif
		+	\| companyURL=http://www.tropicos.org/Image/100007389
		+	\| reference=Tropicos.org. Missouri Botanical Garden. 19 Mar 2014 <http://www.tropicos.org/Image/100007389>
		+
		+	\| }}
		+
	=Organoleptic Characteristics=		=Organoleptic Characteristics=

@@ Line 1: / Line 1: @@
 =Nomenclature=
@@ Line 11: / Line 12: @@
 |notes= }}
-=Macroscopic Entries=
+=Botanical Voucher Specimen=
-[[Category:NoMacroscopy]]
+=Organoleptic Characteristics=
-=Microscopic Entries=
+=Macroscopic Characteristics=
-[[Category:NoMicroscopy]]
+=Microscopic Characteristics=
-=HPTLC Entries=
+=High Performance Thin Layer Chromatographic Identification=
 {{HPTLC | source=HPTLC Association
               | companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
@@ Line 59: / Line 60: @@
               | }}
-=Other Points of Interest=
+=Supplementary Information=
+=Sources=
-[[Category:Blank2]]
+<references />