Monarda didyma (leaf) - Revision history

Admin: add askbox

2014-06-02T20:38:06Z

add askbox

Staffer at 06:20, 26 March 2014

2014-03-26T06:20:56Z

Staffer at 06:18, 26 March 2014

2014-03-26T06:18:40Z

Staffer: Voucher added

2014-03-26T06:17:38Z

Voucher added

Staffer: Various title corrections, Source additions

2014-03-16T01:44:08Z

Various title corrections, Source additions

Staffer: Nomenclature updated

2014-03-14T18:58:08Z

Nomenclature updated

AHPAStaff: addition of hptlc info.--jd

2013-06-25T23:48:07Z

addition of hptlc info.--jd

New page

=Introduction=
[[Category:NoIntro]]

=Macroscopic Entries=
[[Category:NoMacroscopy]]

=Microscopic Entries=
[[Category:NoMicroscopy]]

=HPTLC Entries=
{{HPTLC | source=HPTLC Association
| companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
| companyURL=http://www.hptlc-association.org/
| mainimage=Monarda didyma-NP reagent and PEG reagent, UV 366 nm-hptlc-association.png
| caption1=Oswego tea (leaf) HPTLC ID - NP reagent and PEG reagent, UV 366 nm
| description=Oswego tea (leaf) (''Monarda didyma'')
| image2=Monarda didyma-NP reagent and PEG reagent, white RT-hptlc-association.png
| caption2=Oswego tea (leaf) HPTLC ID - NP reagent and PEG reagent, white RT
|
| stationaryphase=Stationary phase, i.e. Silica gel 60, F254
| mobilephase=Anhydrous formic acid, water, methyl ethyl ketone, ethyl acetate 10:10:30:50 (v/v/v/v)
| prep=Sample: Mix 500 mg of powdered sample with 5 mL of methanol and sonicate for 10 minutes, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions.

Derivatization reagent: 1.) NP reagent, Preparation: 1 g of natural products reagent in 200 mL of ethyl acetate; 2.) PEG reagent, Preparation: 10 g of polyethylene glycol 400 in 200 mL of dichloromethane, Use: Heat plate for 3 min at 100°C, then dip (time 0, speed 5) in NP reagent, dry and dip (time 0, speed 5) in PEG reagent.
| detection=No detection.
| referencesamples=Reference: Individually dissolve 2 mg of rutin and 2 mg of hyperoside each in 10 mL of methanol. Optional: Individually dissolve 4 mg of quercetin, 1.5 mg of chlorogenic acid, 10 mg of naringin, 10 mg of naringenin and 10 mg of narirutin each in 10 mL of methanol. Dissolve 2 mg of rosmarinic acid in 10 mL of ethanol 50% (v/v).
|
| lanes=Lanes, from left to right (Track, Volume, Sample):
# 2 µL Oswego tea; herb 1
# 4 µL Oswego tea; herb 1
# 6 µL Oswego tea; herb 1
# 2 µL Oswego tea; herb with flower
# 4 µL Oswego tea; herb with flower
# 6 µL Oswego tea; herb with flower
# 2 µL Rutin, narirutin, hyperoside, quercetin (with increasing Rf)
# 2 µL Naringin, naringenin (with increasing Rf)
# 2 / 5 µL Chlorogenic acid, rosmarinic acid (with increasing Rf)
# 4 µL Oswego tea; herb 1
# 4 µL Oswego tea; herb 2
# '''4 µL Oswego tea; herb 3'''
# 4 µL Oswego tea; herb 4
# 4 µL Oswego tea; herb with flower
# 4 µL Oswego tea; flower

| notes=''Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.''

System suitability test: Rutin: orange fluorescent zone at Rf ~ 0.32 (UV 366 nm). Hyperoside: orange fluorescent zone at Rf ~ 0.50 (UV 366 nm).

Identification: Compare result under UV 366 nm with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows an intense light blue zone at Rf ~ 0.50 corresponding in position to reference hyperosid and at Rf ~ 0.87 corresponding to reference rosmarinic acid. Faint brownish-yellow zones are seen slightly above the position of rutin and at Rf ~ 0.18 (pink arrows). A greenish grey zone is seen at Rf ~ 0.46 corresponding to the position of reference naringin. Close to the solvent front a red zone is seen. Just below it there is greenish grey zone corresponding to reference substance naringenin (yellow arrow).

Test for other plant parts: Under white RT no red zone is seen at Rf~ 0.25 (black arrow, Oswego tea flower).

| }}

=Other Points of Interest=

[[Category:Blank2]]

← Older revision		Revision as of 20:38, 2 June 2014
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−	{{DISPLAYTITLE:''Monarda didyma'' (leaf) }}	+	{{DISPLAYTITLE:''Monarda didyma'' (leaf) }} {{askbox\|herb=''Monarda didyma''}}
	=Nomenclature=		=Nomenclature=

@@ Line 17: / Line 17: @@
               | source=MOBOT, Tropicos.org
-              | mainimage=Monarda_didyma_Tropicos_100024533.jpg
+              | mainimage=Monarda_didyma_Tropicos_100024136_(S).jpg
               | companyimage=TropicosLogo.gif
-              | companyURL=http://www.tropicos.org/Image/100024533
+              | companyURL=http://www.tropicos.org/Image/100024136
-              | reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/100024533>
+              | reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/100024136>
               | source2=MOBOT, Tropicos.org
-              | image2=Monarda_didyma_Tropicos_100024136_(S).jpg
+              | image2=Monarda_didyma_Tropicos_100024533.jpg
               | companyimage2=TropicosLogo.gif
-              | companyURL2=http://www.tropicos.org/Image/100024136
+              | companyURL2=http://www.tropicos.org/Image/100024533
-              | reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/100024136>
+              | reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/100024533>

@@ Line 14: / Line 14: @@
 =Botanical Voucher Specimen=
-{{Media      |cat=Voucher
+{{Media2      |cat=Voucher
               | source=MOBOT, Tropicos.org
@@ Line 21: / Line 21: @@
               | companyURL=http://www.tropicos.org/Image/100024533
               | reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/100024533>
               | }}

← Older revision		Revision as of 06:17, 26 March 2014
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	=Botanical Voucher Specimen=		=Botanical Voucher Specimen=
		+
		+	{{Media \|cat=Voucher
		+
		+	\| source=MOBOT, Tropicos.org
		+	\| mainimage=Monarda_didyma_Tropicos_100024533.jpg
		+	\| companyimage=TropicosLogo.gif
		+	\| companyURL=http://www.tropicos.org/Image/100024533
		+	\| reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/100024533>
		+
		+	\| }}
		+
		+
	=Organoleptic Characteristics=		=Organoleptic Characteristics=

@@ Line 1: / Line 1: @@
 =Nomenclature=
@@ Line 11: / Line 12: @@
 |notes= }}
-=Macroscopic Entries=
+=Botanical Voucher Specimen=
-[[Category:NoMacroscopy]]
+=Organoleptic Characteristics=
-=Microscopic Entries=
+=Macroscopic Characteristics=
-[[Category:NoMicroscopy]]
+=Microscopic Characteristics=
-=HPTLC Entries=
+=High Performance Thin Layer Chromatographic Identification=
 {{HPTLC | source=HPTLC Association
               | companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
@@ Line 63: / Line 64: @@
-=Other Points of Interest=
+=Supplementary Information=
+=Sources=
-[[Category:Blank2]]
+<references />