Primula veris (flower) - Revision history

Admin: add PlantaPhile references

2015-05-05T15:36:02Z

add PlantaPhile references

Admin: add askbox

2014-06-02T21:01:32Z

add askbox

Staffer at 00:38, 27 March 2014

2014-03-27T00:38:42Z

Staffer: Voucher added

2014-03-26T06:50:47Z

Voucher added

Staffer: Various title corrections, Source additions

2014-03-16T02:01:48Z

Various title corrections, Source additions

Staffer: Nomenclature updated

2014-03-14T21:24:18Z

Nomenclature updated

AHPAStaff: addition of hptlc info.--jd

2013-06-25T00:23:10Z

addition of hptlc info.--jd

New page

=Introduction=
[[Category:NoIntro]]

=Macroscopic Entries=
[[Category:NoMacroscopy]]

=Microscopic Entries=
[[Category:NoMicroscopy]]

=HPTLC Entries=
{{HPTLC | source=HPTLC Association
| companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
| companyURL=http://www.hptlc-association.org/
| mainimage=Primula veris-hptclc-association.png
| caption1=Cowslip Primrose (flower) HPTLC ID - NP reagent and PEG reagent, UV 366 nm
| description=Cowslip Primrose (flower) (''Primula veris'')
|
| stationaryphase=Stationary phase, i.e. Silica gel 60, F254
| mobilephase=Ethyl acetate, water, anhydrous formic acid, acetic acid (100:27:11:11 v/v/v/v)
| prep=Sample: Mix 1 g of powdered sample with 10 mL of methanol, sonicate for 10 minutes, then centrifuge or filter the solution and use the supernatant / filtrate as the test solution.

Derivatization reagent: 1.) NP reagent, Preparation: 1 g of diphenylboric acid 2-aminoethyl ester is dissolved in 200 mL of ethyl acetate; 2.) PEG reagent, Preparation: 10 g of polyethylene glycol (Macrogol) 400 is dissolved in 200 mL of dichloromethane, Use: Heat plate at 100°C for 5 min, then dip (time 0, speed 5) in NP reagent, dry in a stream of cold air, dip (time 0, speed 5) in PEG reagent and dry in a stream of cold air.
| detection=Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33%
| referencesamples=Reference: Dissolve 2 mg of rutin and 2 mg of hyperoside individually in 10 mL of methanol.
|
| lanes=Lanes, from left to right (Track, Volume, Sample):
# 2 µL Rutin
# 2 µL Hyperoside
# '''2 µL ''Primula veris'' flower'''
# 3 µL Rutin
# 3 µL Hyperoside
# 3 µL ''Primula veris'' flower

| notes=''Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.''

System suitability test: Rutin: orange zone at Rf ~ 0.29
Hyperoside: orange zone at Rf ~ 0.49

Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows a green fluorescent zone just above reference substance rutin. At the position of rutin an orange to yellow fluorescent zone is detected. Below the position of rutin there are two orange to yellow fluorescent zones. Below a weak greenish-blue zone is detected and further below a yellow to orange zone is present.

| }}

=Other Points of Interest=

[[Category:Blank2]]

@@ Line 29: / Line 29: @@
 =Macroscopic Characteristics=
 =Microscopic Characteristics=
 =High Performance Thin Layer Chromatographic Identification=
 {{HPTLC | source=HPTLC Association

← Older revision		Revision as of 21:01, 2 June 2014
Line 1:		Line 1:
−	{{DISPLAYTITLE:''Primula veris'' (flower) }}	+	{{DISPLAYTITLE:''Primula veris'' (flower) }} {{askbox\|herb=''Primula veris''}}
	=Nomenclature=		=Nomenclature=

@@ Line 19: / Line 19: @@
               | mainimage=Primula_veris_Tropicos_100105416_(S).jpg
               | companyimage=TropicosLogo.gif
-              | companyURL=http://www.tropicos.org/image/100105416
+              | companyURL=http://www.tropicos.org/Image/100105416
-              | reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/image/100105416>
+              | reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/100105416>
               | }}

← Older revision		Revision as of 06:50, 26 March 2014
Line 13:		Line 13:

	=Botanical Voucher Specimen=		=Botanical Voucher Specimen=
		+
		+	{{Media \|cat=Voucher
		+
		+	\| source=MOBOT, Tropicos.org
		+	\| mainimage=Primula_veris_Tropicos_100105416_(S).jpg
		+	\| companyimage=TropicosLogo.gif
		+	\| companyURL=http://www.tropicos.org/image/100105416
		+	\| reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/image/100105416>
		+
		+	\| }}
		+
		+
	=Organoleptic Characteristics=		=Organoleptic Characteristics=

@@ Line 1: / Line 1: @@
 =Nomenclature=
@@ Line 11: / Line 12: @@
 |notes= }}
-=Macroscopic Entries=
+=Botanical Voucher Specimen=
-[[Category:NoMacroscopy]]
+=Organoleptic Characteristics=
-=Microscopic Entries=
+=Macroscopic Characteristics=
-[[Category:NoMicroscopy]]
+=Microscopic Characteristics=
-=HPTLC Entries=
+=High Performance Thin Layer Chromatographic Identification=
 {{HPTLC | source=HPTLC Association
               | companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
@@ Line 51: / Line 52: @@
-=Other Points of Interest=
+=Supplementary Information=
+=Sources=
-[[Category:Blank2]]
+<references />