Pueraria spp. (root) - Revision history

Admin: adjust source spacing for clarity

2014-06-02T21:02:29Z

adjust source spacing for clarity

Admin: add askbox

2014-06-02T21:02:09Z

add askbox

Admin: add caption to voucher

2014-03-31T19:58:00Z

add caption to voucher

Staffer at 00:35, 27 March 2014

2014-03-27T00:35:25Z

Staffer: Voucher added

2014-03-26T06:51:57Z

Voucher added

Staffer: Various title corrections, Source additions

2014-03-16T02:02:45Z

Various title corrections, Source additions

AHPAStaff: addition of hptlc info.--jd

2013-06-25T01:05:56Z

addition of hptlc info.--jd

New page

=Introduction=
[[Category:NoIntro]]

=Macroscopic Entries=
[[Category:NoMacroscopy]]

=Microscopic Entries=
[[Category:NoMicroscopy]]

=HPTLC Entries=
{{HPTLC | source=HPTLC Association
| companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
| companyURL=http://www.hptlc-association.org/
| mainimage=Pueraria spp-hptclc-association.png
| caption1=Kudzu root, ge gen (root) HPTLC ID - UV 254 nm
| description=Kudzu root, ge gen (root) (''Pueraria montana'' var. ''lobata'')
|
| stationaryphase=Stationary phase, i.e. Silica gel 60, F254
| mobilephase=Dichloromethane, ethyl acetate, methanol, water 20:40:22:10 (v/v/v/v)--NOTE: to avoid the formation of two phases respect this order when mixing the mobile phase.
| prep=Sample: Mix 500 mg of powdered sample with 5 mL of methanol and sonicate for 10 minutes, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions.

| detection=Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33%
| referencesamples=Reference: Dissolve 1 mg of daidzin and 1 mg of puerarin individually in 1 mL of methanol. Optional: Dissolve 1 mg of daidzein in 1 mL of methanol.
|
| lanes=Lanes, from left to right (Track, Volume, Sample):
# 7 µL Kudzu root #1
# '''7 µL Kudzu root #2'''
# 7 µL Kudzu root #3
# 7 µL Kudzu root #4
# 7 µL Kudzu root #5
# 7 µL Puerarin
# 7 µL Daidzin, Daidzein (with increasing Rf)
# 7 µL Kudzu root #6
# 7 µL Thomson’s kudzu root #1
# 7 µL Thomson’s kudzu root #2
# 7 µL Kudzu root #7 (mislabeled)
# 7 µL Thomson’s kudzu root #3
# 7 µL Thomson’s kudzu root #4
# 7 µL Thomson’s kudzu root #5
# 7 µL Thomson’s kudzu root #6

| notes=''Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.''

System suitability test: Puerarin: dark quenching zone at Rf ~ 0.48; Daidzin: dark quenching zone at Rf ~ 0.51

Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows intense quenching zones corresponding to reference substances puerarin and daidzin (red arrows). Below the zone corresponding to puerarin there are at least 5 quenching zones of medium intensity (orange arrow). Above the zone corresponding to daidzin there are 1 to 2 distinct quenching zones. At Rf ~ 0.75 there is a weak zone corresponding to reference standard daidzein.

Test for other species: The chromatogram of Thomson’s kudzu root shows only two weak quenching zones corresponding to reference substances puerarin and daidzin.

| }}

{{HPTLC | source=HPTLC Association
| companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
| companyURL=http://www.hptlc-association.org/
| mainimage=Pueraria montana var. chinensis-hptlc-association.png
| caption1=Thomson’s kudzu root, gen ge (root) HPTLC ID - UV 254 nm
| description=Thomson’s kudzu root, gen ge (root) (''Pueraria montana'' var. ''chinensis'')
|
| stationaryphase=Stationary phase, i.e. Silica gel 60, F254
| mobilephase=Dichloromethane, ethyl acetate, methanol, water 20:40:22:10 (v/v/v/v); NOTE: to avoid the formation of two phases respect this order when mixing the mobile phase.
| prep=Sample: Mix 500 mg of powdered sample with 5 mL of methanol and sonicate for 10 minutes, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions.

| detection=Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33%
| referencesamples=Reference: Dissolve 1 mg of daidzin and 1 mg of puerarin individually in 1 mL of methanol. Optional: Dissolve 1 mg of daidzein in 1 mL of methanol.
|
| lanes=Lanes, from left to right (Track, Volume, Sample):
# 7 µL Kudzu root 1
# 7 µL Kudzu root 2
# 7 µL Kudzu root 3
# 7 µL Kudzu root 4
# 7 µL Kudzu root 5
# 7 µL Puerarin
# 7 µL Daidzin, Daidzein (with increasing Rf)
# 7 µL Kudzu root 6
# 7 µL Thomson’s kudzu root 1
# '''7 µL Thomson’s kudzu root 2'''
# 7 µL Kudzu root 7 (mislabeled)
# 7 µL Thomson’s kudzu root 3
# 7 µL Thomson’s kudzu root 4
# 7 µL Thomson’s kudzu root 5
# 7 µL Thomson’s kudzu root 6

| notes=''Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.''

System suitability test: Puerarin: dark quenching zone at Rf ~ 0.48; Daidzin: dark quenching zone at Rf ~ 0.51.

Identification: Compare result with reference image. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows weak quenching zones corresponding to reference standards puerarin and daidzin (black arrows). Below the zone corresponding to puerarin there are no or only very weak quenching zones. At Rf 0.75 there may be a weak zone corresponding to reference standard daidzein.

Test for other species: No intense zone is seen between the zone corresponding to puerarin and the start position (red arrow, Kudzu root).

| }}

=Other Points of Interest=

[[Category:Blank2]]

@@ Line 60: / Line 60: @@
               | }}
 {{HPTLC | source=HPTLC Association
@@ Line 101: / Line 102: @@
               | }}
 =Supplementary Information=
 =Sources=
 <references />

← Older revision		Revision as of 21:02, 2 June 2014
Line 1:		Line 1:
−	{{DISPLAYTITLE:''Pueraria'' spp. (root) }}	+	{{DISPLAYTITLE:''Pueraria'' spp. (root) }} {{askbox\|herb=''Pueraria'' spp.}}
	=Nomenclature=		=Nomenclature=

@@ Line 11: / Line 11: @@
               | companyURL=http://www.tropicos.org/Image/81216
               | reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/81216>
+|caption1=''Pueraria phaseoloides''
               | }}

@@ Line 9: / Line 9: @@
               | mainimage=Pueraria_phaseoloides_Tropicos_81216_(S).jpg
               | companyimage=TropicosLogo.gif
-              | companyURL=http://www.tropicos.org/image/81216
+              | companyURL=http://www.tropicos.org/Image/81216
-              | reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/image/81216>
+              | reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/81216>
               | }}

← Older revision		Revision as of 06:51, 26 March 2014
Line 3:		Line 3:

	=Botanical Voucher Specimen=		=Botanical Voucher Specimen=
		+
		+	{{Media \|cat=Voucher
		+
		+	\| source=MOBOT, Tropicos.org
		+	\| mainimage=Pueraria_phaseoloides_Tropicos_81216_(S).jpg
		+	\| companyimage=TropicosLogo.gif
		+	\| companyURL=http://www.tropicos.org/image/81216
		+	\| reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/image/81216>
		+
		+	\| }}
		+
	=Organoleptic Characteristics=		=Organoleptic Characteristics=

@@ Line 1: / Line 1: @@
-=Introduction=
+{{DISPLAYTITLE:''Pueraria'' spp. (root) }}
-[[Category:NoIntro]]
+=Nomenclature=
-=Macroscopic Entries=
+=Botanical Voucher Specimen=
-[[Category:NoMacroscopy]]
+=Organoleptic Characteristics=
-=Microscopic Entries=
+=Macroscopic Characteristics=
-[[Category:NoMicroscopy]]
+=Microscopic Characteristics=
-=HPTLC Entries=
+=High Performance Thin Layer Chromatographic Identification=
 {{HPTLC | source=HPTLC Association
               | companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
@@ Line 92: / Line 92: @@
-=Other Points of Interest=
+=Supplementary Information=
+=Sources=
-[[Category:Blank2]]
+<references />