Rosmarinus officinalis (leaf) - Revision history

Admin: add PlantaPhile references

2015-04-28T18:30:15Z

add PlantaPhile references

Admin: add USD 1918 information (organoleptic and macroscopic)

2015-04-07T15:09:10Z

add USD 1918 information (organoleptic and macroscopic)

Admin: add askbox

2014-06-02T21:06:04Z

add askbox

Staffer at 00:39, 27 March 2014

2014-03-27T00:39:05Z

Staffer: Voucher added

2014-03-26T06:53:44Z

Voucher added

Staffer: New layout

2014-03-17T03:49:48Z

New layout

Staffer: Various title corrections, Source additions

2014-03-16T02:22:18Z

Various title corrections, Source additions

Staffer: Nomenclature updated

2014-03-14T21:33:55Z

Nomenclature updated

AHPAStaff: addition of hptlc info.--jd

2013-06-21T18:54:52Z

addition of hptlc info.--jd

JKim at 06:52, 23 January 2013

2013-01-23T06:52:52Z

@@ Line 36: / Line 36: @@
 {{Macroscopy | source=United States Dispensatory (1918)
 | description=Rosemary is a small evergreen shrub, with an erect stem, divided into many long, slender, ash-colored branches. The leaves are numerous, sessile, opposite, about 2.5 cm. long, rigid, linear, entire, obtuse at the summit, folded backward at the edges, of a firm consistence, smooth and green on the upper surface, whitish, woolly, and glandular beneath. The flowers are axillary, pale blue or white.}}
 |}
 =Microscopic Characteristics=

@@ Line 24: / Line 24: @@
 =Organoleptic Characteristics=
 =Macroscopic Characteristics=
 =Microscopic Characteristics=
 {{Media2 |cat=Microscopy | source=Elan M. Sudberg, Alkemist Laboratories

← Older revision		Revision as of 21:06, 2 June 2014
Line 1:		Line 1:
−	{{DISPLAYTITLE:''Rosmarinus officinalis'' (leaf) }}	+	{{DISPLAYTITLE:''Rosmarinus officinalis'' (leaf) }} {{askbox\|herb=''Rosmarinus officinalis''}}
	=Nomenclature=		=Nomenclature=

@@ Line 18: / Line 18: @@
               | mainimage=Rosmarinus_officinalis_Tropicos_100002347_(S).jpg
               | companyimage=TropicosLogo.gif
-              | companyURL=http://www.tropicos.org/image/100002347
+              | companyURL=http://www.tropicos.org/Image/100002347
-              | reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/image/100002347>
+              | reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/100002347>
               | }}

@@ Line 12: / Line 12: @@
 |notes= }}
 =Botanical Voucher Specimen=
 =Organoleptic Characteristics=

@@ Line 16: / Line 16: @@
 =Macroscopic Characteristics=
 =Microscopic Characteristics=
-{{Microscopy | source=Elan M. Sudberg, Alkemist Laboratories
+{{Media2 |cat=Microscopy | source=Elan M. Sudberg, Alkemist Laboratories
         | companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
-        | companyURL=http://www.Alkemist.com
+        | companyURL=http://www.alkemist.com
         | mainimage=Rosemary Alkemist Laboratories.jpg
         | caption1=Branched covering trichomes from the lower surface of the leaf observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
-        | description= Rosemary (leaf) (''Rosmarinus officinalis'')
+| source2=Elan M. Sudberg, Alkemist Laboratories
         | image2=Rosemary-1 Alkemist Laboratories.jpg
         | caption2=Large glandular scales observed at 400x with Acidified Chloral Hydrate Glycerol Solution.

@@ Line 1: / Line 1: @@
 =Nomenclature=
@@ Line 10: / Line 11: @@
 |aka=
 |notes= }}
-=Macroscopic Entries=
+=Macroscopic Characteristics=
-=Microscopic Entries=
+=Microscopic Characteristics=
 {{Microscopy | source=Elan M. Sudberg, Alkemist Laboratories
         | companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
@@ Line 26: / Line 29: @@
-=HPTLC Entries=
+=High Performance Thin Layer Chromatographic Identification=
 {{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
               | description=Rosemary (leaf) (''Rosmarinus officinalis'')
@@ Line 98: / Line 101: @@
-=Other Points of Interest=
+=Supplementary Information=
-[[Category:NoIntro]]
+=Sources=

← Older revision		Revision as of 18:54, 21 June 2013
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	\| reference=British Pharmacopoeia, 2003		\| reference=British Pharmacopoeia, 2003
		+	\| }}
		+
		+	{{HPTLC \| source=HPTLC Association
		+	\| companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
		+	\| companyURL=http://www.hptlc-association.org/
		+	\| mainimage=Rosmarinus officinalis-hptlc-association.png
		+	\| caption1=Rosemary (leaf) HPTLC ID - NP and PEG reagent, UV 366 nm
		+	\| description=Rosemary (leaf) (''Rosmarinus officinalis'')
		+	\|
		+	\| stationaryphase=Stationary phase, i.e. Silica gel 60, F254
		+	\| mobilephase=Ethyl acetate, formic acid, water 15:1:1 (v/v/v)
		+	\| prep=Sample: Mix 1 g of powdered sample with 10 mL of methanol and sonicate for 10 minutes, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions.
		+
		+	Derivatization reagent: 1.) NP reagent
		+	Preparation: 1 g of natural products reagent in 200 mL of ethyl acetate
		+	2.) PEG reagent
		+	Preparation: 10 g of polyethylene glycol 400 in 200 mL of dichloromethane
		+	Use: Heat plate 3 min at 100°C, then dip (time 0, speed 5) in NP reagent, dry and dip (time 0, speed 5) in PEG reagent
		+	\| detection=Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33%
		+	\| referencesamples=Reference: Dissolve 5 mg of rosmarinic acid in 10 mL of methanol. Dissolve 6 mg of caffeic acid in 10 mL of methanol.
		+	\|
		+	\| lanes=Lanes, from left to right (Track, Volume, Sample):
		+	# 4 µL Rosemary leaf
		+	# '''8 µL Rosemary leaf'''
		+	# 16 µL Rosemary leaf
		+	# 1 µL Rosemary leaf 1 (Ph. Eur extract.)
		+	# 2 µL Rosemary leaf 1 (Ph. Eur extract.)
		+	# 4 µL Rosemary leaf 1 (Ph. Eur extract.)
		+	# 2 µL Rosmarinic acid
		+	# 2 µL Caffeic acid
		+	# 8 µL Oregano leaf (old sample)
		+	# 8 µL Rosemary leaf 2
		+	# 8 µL Rosemary leaf 3
		+	# 8 µL Thyme leaf
		+	# 8 µL Holy basil leaf
		+	# 8 µL Sweet basil leaf
		+	# 8 µL Marjoram leaf
		+
		+	\| notes=''Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.''
		+
		+	System suitability test: Rosmarinic acid: light blue fluorescent zone at Rf ~ 0.73. Caffeic acid: light blue fluorescent zone at Rf ~ 0.76.
		+
		+	Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows a red zone right below the solvent front. At the position of reference substance rosmarinic acid an intense light blue zone is seen. A green zone is detected at Rf ~ 0.50 (blue arrow). Below this zone there is a pattern of characteristically colored zones of low intensity (pink arrow). An intense orange zone is detected at Rf ~ 0.21 (yellow arrow).
		+
		+	Test for other species: There are no two orange zones at Rf ~ 0.27 and 0.20 (red arrows, Oregano leaf or Thyme leaf). No light blue zone is seen at Rf ~ 0.32 (white arrow, Holy basil leaf). No orange zone is seen at Rf ~ 0.27 and no zone is seen at Rf ~ 0.63 (green arrows, Sweet basil leaf).
	\| }}		\| }}

← Older revision		Revision as of 06:52, 23 January 2013
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	=Other Points of Interest=		=Other Points of Interest=
−	[[Category:~~blank~~]]	+	[[Category:NoIntro]]