Salvia officinalis (leaf) - Revision history

Admin: add PlantaPhile references, typesetting

2015-04-28T18:26:56Z

add PlantaPhile references, typesetting

Admin: add USD 1918 information (organoleptic and macroscopic)

2015-04-07T17:19:09Z

add USD 1918 information (organoleptic and macroscopic)

Admin: add askbox

2014-06-02T21:09:41Z

add askbox

Staffer at 00:40, 27 March 2014

2014-03-27T00:40:14Z

Staffer: Voucher added

2014-03-26T06:55:46Z

Voucher added

Staffer: Various title corrections, Source additions

2014-03-16T02:30:38Z

Various title corrections, Source additions

Staffer: Nomenclature updated

2014-03-14T21:38:58Z

Nomenclature updated

AHPAStaff: addition of hptlc info.--jd

2013-06-26T00:03:02Z

addition of hptlc info.--jd

New page

=Introduction=
[[Category:NoIntro]]

=Macroscopic Entries=
[[Category:NoMacroscopy]]

=Microscopic Entries=
[[Category:NoMicroscopy]]

=HPTLC Entries=
{{HPTLC | source=HPTLC Association
| companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
| companyURL=http://www.hptlc-association.org/
| mainimage=Salvia officinalis-hptlc-association.png
| caption1=Sage (leaf) HPTLC ID - Anisaldehyde reagent, white RT
| description=Sage (leaf) (''Salvia officinalis'')
|
| stationaryphase=Stationary phase, i.e. Silica gel 60, F254
| mobilephase=Dichloromethane
| prep=Sample: Mix 500 mg of powdered sample with 5 mL of methanol and sonicate for 10 minutes, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions.

Derivatization reagent: Anisaldehyde reagent, Preparation: 170 mL of ice-cooled methanol add 20 mL of glacial acetic acid, 10 mL of sulfuric acid and 1 mL of anisaldehyde, Use: spray, heat at 100°C for 5 min
| detection=Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33%
| referencesamples=Reference: Dissolve 5 mg of thujon in 1 mL of methanol; Dissolve 25 µL of cineole in 10 mL of toluene.
|
| lanes=Lanes, from left to right (Track, Volume, Sample):
# 3µL Sage oil
# 3µL Clary sage oil
# 10µL Cineole, thujon (with increasing Rf)
# 5µL Sage leaf
# '''7µL Sage leaf'''
# 10µL Sage leaf
# 5µL Clary sage leaf
# 7µL Clary sage leaf
# 10µL Clary sage leaf
# 5µL Spanish sage leaf
# 7µL Spanish sage leaf
# 10µL Spanish sage leaf
# 5µL Three-lobed sage leaf
# 7µL Three-lobed sage leaf
# 10µL Three-lobed sage leaf

| notes=''Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.''

System suitability test: Thujon: violet zone at Rf ~ 0.47; Cineole: violet zone at Rf ~ 0.27.

Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows a violet zone just below the solvent front. A weak yellow zone is seen at Rf ~ 0.50 (green arrow). There is a violet zone at Rf ~ 0.30 and slightly below reference cineole there is another violet zone. A yellow zone is seen at Rf ~ 0.20 and there is a blue violet zone just below it.

Test for other species: No violet zone is seen at the position of thujon (Three-lobed sage leaf, blue arrow).

| }}

{{HPTLC | source=HPTLC Association
| companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
| companyURL=http://www.hptlc-association.org/
| mainimage=Salvia officinalis oil-hptlc-association.png
| caption1=Sage oil (leaf) HPTLC ID - Anisaldehyde reagent, white RT
| description=Sage oil (leaf) (''Salvia officinalis'')
|
| stationaryphase=Stationary phase, i.e. Silica gel 60, F254
| mobilephase=Dichloromethane
| prep=Sample: Mix 100 µL of sample with 10 mL of toluene.

Derivatization reagent: Anisaldehyde reagent, Preparation: To 170 mL of ice-cooled methanol add 20 mL of acetic acid, 10 mL of sulfuric acid and 1 mL of anisaldehyde, Use: spray, heat at 100°C for 5 min.
| detection=Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33%
| referencesamples=Reference: Dissolve 1 mg of thujon in 1 mL of toluene. Dissolve 30 µL of cineole in 10 mL of toluene.
|
| lanes=Lanes, from left to right (Track, Volume, Sample):
# 7µL Sage leaf
# 7µL Clary sage leaf
# 7µL Spanish sage leaf
# 7µL Three-lobe sage leaf
# 2µL Thujon
# 2µL Cineol
# 1µL Sage oil
# '''3µL Sage oil'''
# 5µL Sage oil
# 1µL Clary sage oil
# 3µL Clary sage oil
# 5µL Clary sage oil
# 1µL Spanish sage oil
# 3µL Spanish sage oil
# 5µL Spanish sage oil

| notes=''Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.''

System suitability test: Thujon: weak pink zone at Rf ~ 0.47; Cineole: violet zone at Rf ~ 0.27.

Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows a grey zone at Rf ~ 0.54. At the position of reference thujon there is a diffuse pink zone (black arrow). Below it there is another pink zone. Just below the position of reference cineole a violet zone is seen. Below this zone there is an intense grey zone at Rf ~ 0.20.

Test for other species: No zone is seen below the grey zone at Rf ~ 0.20 (green arrow, Clary sage oil). The chromatograms of Spanish sage oil and Clary sage oil lack the zone at the position of thujon.

| }}

=Other Points of Interest=

[[Category:Blank2]]

@@ Line 35: / Line 35: @@
 {{Macroscopy | source=United States Dispensatory (1918)
 | description=[''Salvia officinalis''] is a perennial plant, about two feet high, with a quadrangular, pubescent, branching, shrubby stem, furnished with opposite, petiolate, ovate-lanceolate, crenulate, wrinkled leaves, of a grayish-green color, sometimes tinged with red or purple. The flowers are blue, variegated with white and purple, and are disposed on long terminal spikes, in distant whorls, each composed of a few flowers, and provided with ovate, acute, deciduous bracts. The calyx is tubular and striated, with two lips, of which the upper has three acute teeth, the under two. The corolla is tubular, bilabiate, ringent, with the upper lip concave, and the lower divided into three rounded lobes, of which the middle is the largest. The filaments are supported upon short pedicels, to which they are affixed transversely at the middle. [Leaves] long and stoutly petiolate, the blade elliptical or ovate-oblong, 3 to 7 Cm. long, obtuse or subacute at the summit, rounded or subcordate at the base, finely crenulate, thick, grayish-green, very pubescent, especially on the under surface, conspicuously reticulate-veined ...}}
 |}
@@ Line 81: / Line 92: @@
               | }}
 {{HPTLC | source=HPTLC Association

@@ Line 24: / Line 24: @@
 =Organoleptic Characteristics=
 =Macroscopic Characteristics=
 =Microscopic Characteristics=

@@ Line 1: / Line 1: @@
-{{DISPLAYTITLE:''Salvia officinalis'' (leaf) }}
+{{DISPLAYTITLE:''Salvia officinalis'' (leaf) }} {{askbox|herb=''Salvia officinalis''}}
-−
 =Nomenclature=
 {{nomenclature | binomial=Salvia officinalis
 |authority=L.

@@ Line 20: / Line 20: @@
               | mainimage=Salvia_officinalis_Tropicos_100268968_(S).jpg
               | companyimage=TropicosLogo.gif
-              | companyURL=http://www.tropicos.org/image/100268968
+              | companyURL=http://www.tropicos.org/Image/100268968
-              | reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/image/100268968>
+              | reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/100268968>
               | }}

← Older revision		Revision as of 06:55, 26 March 2014
Line 14:		Line 14:

	=Botanical Voucher Specimen=		=Botanical Voucher Specimen=
		+
		+	{{Media \|cat=Voucher
		+
		+	\| source=MOBOT, Tropicos.org
		+	\| mainimage=Salvia_officinalis_Tropicos_100268968_(S).jpg
		+	\| companyimage=TropicosLogo.gif
		+	\| companyURL=http://www.tropicos.org/image/100268968
		+	\| reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/image/100268968>
		+
		+	\| }}
		+
	=Organoleptic Characteristics=		=Organoleptic Characteristics=

@@ Line 1: / Line 1: @@
 =Nomenclature=
@@ Line 11: / Line 13: @@
 |notes= }}
-=Macroscopic Entries=
+=Botanical Voucher Specimen=
-[[Category:NoMacroscopy]]
+=Organoleptic Characteristics=
-=Microscopic Entries=
+=Macroscopic Characteristics=
-[[Category:NoMicroscopy]]
+=Microscopic Characteristics=
-=HPTLC Entries=
+=High Performance Thin Layer Chromatographic Identification=
 {{HPTLC | source=HPTLC Association
               | companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
@@ Line 102: / Line 104: @@
               | }}
-=Other Points of Interest=
+=Supplementary Information=
+=Sources=
-[[Category:Blank2]]
+<references />