Saposhnikovia divaricata (root) - Revision history

Admin: add askbox

2014-06-02T21:15:47Z

add askbox

Staffer: Various title corrections, Source additions

2014-03-16T02:32:26Z

Various title corrections, Source additions

Staffer: Nomenclature updated

2014-03-14T21:42:05Z

Nomenclature updated

AHPAStaff: template edit - jd

2013-06-26T00:06:40Z

template edit - jd

New page

=Introduction=
[[Category:NoIntro]]

=Macroscopic Entries=
[[Category:NoMacroscopy]]

=Microscopic Entries=
[[Category:NoMicroscopy]]

=HPTLC Entries=
{{HPTLC | source=HPTLC Association
| companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
| companyURL=http://www.hptlc-association.org/
| mainimage=Saposhnikovia divaricata-hptlc-association.png
| caption1=Saposhnikovia root, Fang feng (root) HPTLC ID - UV 366 nm
| description=Saposhnikovia root, Fang feng (root) (''Saposhnikovia divaricata'')
|
| stationaryphase=Stationary phase, i.e. Silica gel 60, F254
| mobilephase=Dichloromethane, methanol, acetonitrile 4:2:1 (v/v/v)
| prep=Sample: Mix 1.0 g of powdered sample with 10 mL of methanol and sonicate for 30 minutes, then centrifuge or filter the solutions and use the supernatant / filtrate as test solution.

| detection=Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33%
| referencesamples=Reference: Dissolve 1.0 mg of Prim-O-glucosylcimifugin in 1 mL of methanol. Dissolve 1.0mg of 4'-O-β-D-Glucosyl-5-O- methylvisamminol acid in 1 mL of methanol.
|
| lanes=Lanes, from left to right (Track, Volume, Sample):
# 7.5 µL Prim-O-glucosylcimifugin
# 7.5 µL 4'-O-β-D-Glucosyl-5-O-methylvisamminol
# '''7.5 µL Saposhnikovia root, Chinese 1, BRM'''
# '''7.5 µL Saposhnikovia root, Chinese 2'''
# '''7.5 µL Saposhnikovia root, Chinese 3'''
# '''7.5 µL Saposhnikovia root, Chinese 4'''
# '''7.5 µL Saposhnikovia root, Chinese 5'''
# '''7.5 µL Saposhnikovia root, Chinese 6'''
# '''7.5 µL Saposhnikovia root, German'''
# 7.5 µL Caraway root
# 7.5 µL Wild Carrot root
# 7.5 µL ''Seseli mairei'' root

| notes=''Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.''

System suitability test: Prim-O-glucosylcimifugin: fluorescent zone at Rf ~ 0.41; 4'-O-β-D-Glucosyl-5-O-methylvisamminol: fluorescent zone at Rf ~ 0.51.

Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows blue fluorescent zones at Rf ~ 0.41 and Rf ~ 0.51 corresponding to reference substances prim-O-glucosylcimifugin and 4'-O-β-D-Glucosyl-5-O-methylvisamminol (yellow arrows). Several blue fluorescent zones are detected above the position of reference substance 4'-O-β-D-Glucosyl-5-O-methylvisamminol.

Test for adulteration: Caraway root, Wild Carrot root and Seseli mairei root show no blue zone at the position of reference substances prim-O-glucosylcimifugin and 4'-O-β-D-Glucosyl-5-O-methylvisamminol.

| }}

=Other Points of Interest=

[[Category:Blank2]]

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