Serenoa repens (fruit) - Revision history

Admin: add category marker for DNA category

2015-10-30T14:02:31Z

add category marker for DNA category

Admin: add macro characteristics

2015-06-24T15:56:36Z

add macro characteristics

Admin: correct typesetting

2015-04-07T20:34:32Z

correct typesetting

Admin: add USD 1918 information (organoleptic, macro, micro)

2015-04-07T20:32:51Z

add USD 1918 information (organoleptic, macro, micro)

Admin: correct logo for DNA

2014-09-24T14:15:44Z

correct logo for DNA

Admin at 17:44, 23 September 2014

2014-09-23T17:44:48Z

Admin: add logo (TEMPORARY)

2014-09-23T17:44:27Z

add logo (TEMPORARY)

Admin at 15:16, 22 September 2014

2014-09-22T15:16:49Z

Admin at 15:14, 22 September 2014

2014-09-22T15:14:22Z

Admin at 14:10, 22 September 2014

2014-09-22T14:10:18Z

@@ Line 67: / Line 67: @@
 |}
 =DNA Identification=
 {| border=1
 |[[File:NY_Botanical_Garden_logo.png|right|150x75px]]

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 {| border=1
+|
 {{Macroscopy | source=United States Dispensatory (1918)
 | description=The stem of saw palmetto is from 6 to 10 feet high, has a crown of large leave's, long petioled, with a circular fan-shaped leaf blade split at the edge into from 15 to 30 divisions, which are slightly cleft at their apices. The fruit is a onesided blackish-brown drupe, from half an inch to one inch long; ovoid-oblong in shape, with a somewhat wrinkled exterior [...] It occurs in a large panicle which in, some cases may weigh as much as nine pounds.}}

@@ Line 44: / Line 44: @@
 | description=The powder is yellowish-brown; when examined under the microscope it exhibits large, irregular fragments; parenchyma cells of sarcocarp containing a yellowish-brown or brownish-red, amorphous substance; whitish fragments of endosperm; the walls being considerably thickened and with large pores; stone cells occasional, nearly colorless, more or less tabular or irregular in shape, about 0.125 mm. in length, walls about 0.015 mm. in thickness, and with numerous simple or branching pores.}}
 {{Microscopy | source=Elan M. Sudberg, Alkemist Laboratories
               | companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
@@ Line 64: / Line 62: @@
             |caption2= Cells of the sarcocarp filled with reddish brown pigment observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
             |source2= Elan M. Sudberg, Alkemist Laboratories }}
 |}
 =DNA Identification=

@@ Line 14: / Line 14: @@
 =Organoleptic Characteristics=
 =Macroscopic Characteristics=
 {{Media2 |cat=Macroscopy
 | source=PlantaPhile
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               | companyURL2=http://plantaphile.com/
               | }}
 =Microscopic Characteristics=
 {| border=1
+|
@@ Line 49: / Line 65: @@
             |source2= Elan M. Sudberg, Alkemist Laboratories }}
 |}
+|}
 =DNA Identification=
 {| border=1

@@ Line 52: / Line 52: @@
 =DNA Identification=
 {| border=1
-|[[File:Starr herbarium logo.png|right|150x75px]]
+|[[File:NY_Botanical_Garden_logo.png|right|150x75px]]
 '''Saw Palmetto (''Serenoa repens'')'''

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 '''PCR reaction mixture:'''
-''matK'' mini–barcode: 15 µL final volume containing 1.5 µL PCR buffer (200 mM tris [pH 8.8], 100 mM KCl, 100 mM (NH<sub>4</sub>)2SO<sub>4</sub>, 20 mM MgSO<sub>4</sub>, 1% [v/v] Triton X-100, 50% [w/v] sucrose, 0.25% [w/v] cresol red), 0.2 µM dNTPs, 0.025 μg/μL bovine serum albumin (BSA), 1 μM of each primer, 0.25 units of ''Taq'' polymerase, and 0.5 µL template DNA.
+''matK'' mini–barcode: 15 µL final volume containing 1.5 µL PCR buffer (200 mM tris [pH 8.8], 100 mM KCl, 100 mM (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>, 20 mM MgSO<sub>4</sub>, 1% [v/v] Triton X-100, 50% [w/v] sucrose, 0.25% [w/v] cresol red), 0.2 µM dNTPs, 0.025 μg/μL bovine serum albumin (BSA), 1 μM of each primer, 0.25 units of ''Taq'' polymerase, and 0.5 µL template DNA.
-''rbcL'' mini–barcode: 15 µL final volume containing 1.5 µL PCR buffer (200 mM tris [pH 8.8], 100 mM KCl, 100 mM (NH<sub>4</sub>)2SO<sub>4</sub>, 20 mM MgSO<sub>4</sub>, 1% [v/v] Triton X-100, 50% [w/v] sucrose, 0.25% [w/v] cresol red), 0.2 µM dNTPs, 0.025 μg/μL bovine serum albumin (BSA), 48 mM betaine, 1 μM of each primer, 0.25 units of ''Taq'' polymerase, and 0.5 µL template DNA.
+''rbcL'' mini–barcode: 15 µL final volume containing 1.5 µL PCR buffer (200 mM tris [pH 8.8], 100 mM KCl, 100 mM (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>, 20 mM MgSO<sub>4</sub>, 1% [v/v] Triton X-100, 50% [w/v] sucrose, 0.25% [w/v] cresol red), 0.2 µM dNTPs, 0.025 μg/μL bovine serum albumin (BSA), 48 mM betaine, 1 μM of each primer, 0.25 units of ''Taq'' polymerase, and 0.5 µL template DNA.
 '''PCR program:''' 95°C for 2.5 min; 35 cycles: 95°C for 30 s, 60°C for 30 s; 60°C for 10 min.

@@ Line 51: / Line 51: @@
 =DNA Identification=
 '''Saw Palmetto (''Serenoa repens'')'''
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 Serenoa repens                    ......
 </pre>
 =Supplementary Information=