Solidago gigantea (aerial parts) - Revision history

Admin: add askbox

2014-06-02T21:19:50Z

add askbox

Staffer: Vouchers added

2014-03-27T02:50:14Z

Vouchers added

Staffer: Various title corrections, Source additions

2014-03-16T02:39:35Z

Various title corrections, Source additions

Staffer: Nomenclature updated

2014-03-14T21:53:23Z

Nomenclature updated

AHPAStaff: addition of hptlc info.--jd

2013-06-25T01:04:10Z

addition of hptlc info.--jd

New page

=Introduction=
[[Category:NoIntro]]

=Macroscopic Entries=
[[Category:NoMacroscopy]]

=Microscopic Entries=
[[Category:NoMicroscopy]]

=HPTLC Entries=
{{HPTLC | source=HPTLC Association
| companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
| companyURL=http://www.hptlc-association.org/
| mainimage=Solidago gigantea-hptclc-association.png
| caption1=Giant goldenrod herb (aerial parts) HPTLC ID - NP reagent and PEG 400 reagent, UV 366 nm
| description=Giant goldenrod herb (aerial parts) (''Solidago gigantea'')
|
| stationaryphase=Stationary phase, i.e. Silica gel 60, F254
| mobilephase=
| prep=Sample: Mix 500 mg of powdered sample with 5 mL of methanol and sonicate for 10 minutes, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions.

Derivatization reagent: 1.) NP reagent, Preparation: 1 g of natural products reagent in 200 mL of ethyl acetate; 2.) PEG reagent, Preparation: 10 g of polyethylene glycol 400 in 200 mL of dichloromethane, Use: Heat plate for 3 min at 100°C, then dip (time 0, speed 5) in NP reagent, dry and dip (time 0, speed 5) in PEG reagent.
| detection=Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33%
| referencesamples=Reference: Individually dissolve 1 mg of rutin and 1 mg of chlorogenic acid each in 1 mL of methanol. Optional: dissolve 1 mg of quercitrin in 1 mL of methanol.
|
| lanes=Lanes, from left to right (Track, Volume, Sample):
# 3 µL Giant goldenrod herb 1
# '''5 µL Giant goldenrod herb 1'''
# 7 µL Giant goldenrod herb 1
# 1 µL Giant goldenrod herb 1 (Ph.Eur. extraction)
# 3 µL Giant goldenrod herb 1 (Ph.Eur. extraction)
# 5 µL Giant goldenrod herb 1 (Ph.Eur. extraction)
# 5 µL Rutin
# 5 µL Quercitrin
# 5 µL Chlorogenic acid
# 5 µL Giant goldenrod herb 2
# 5 µL Goldenrod herb (old sample)
# 7 µL Goldenrod herb (old sample)
# 5 µL European goldenrod herb 1
# 7 µL European goldenrod herb 1

| notes=''Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.''

System suitability test: Rutin: orange fluorescent zone at Rf ~ 0.30; Chlorogenic acid: green fluorescent zone at Rf ~ 0.48.

Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows an orange zone at Rf ~ 0.30 corresponding to reference substance rutin and a green zone at Rf ~ 0.48 corresponding to chlorogenic acid (pink arrows). Above it there are several faint brown zones and an intense brown zone (Rf ~ 0.67) at the position of quercitrin (yellow arrow). A green zone is seen at Rf ~ 0.82.

Test for other species: European goldenrod herb does not show an intense brown zone at Rf ~ 0.67.

| }}

=Other Points of Interest=

[[Category:Blank2]]

← Older revision		Revision as of 21:19, 2 June 2014
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−	{{DISPLAYTITLE:''Solidago gigantea'' (aerial parts) }}	+	{{DISPLAYTITLE:''Solidago gigantea'' (aerial parts) }} {{askbox\|herb=''Solidago gigantea''}}
	=Nomenclature=		=Nomenclature=

← Older revision		Revision as of 02:50, 27 March 2014
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	=Botanical Voucher Specimen=		=Botanical Voucher Specimen=
		+
		+	{{Media2 \| cat=Voucher
		+
		+	\| source=MOBOT, Tropicos.org
		+	\| mainimage=Solidago_gigantea_Tropicos_89552.jpg
		+	\| companyimage=TropicosLogo.gif
		+	\| companyURL=http://www.tropicos.org/Image/89552
		+	\| reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/89552>
		+
		+	\| source2=MOBOT, Tropicos.org
		+	\| image2=Solidago_gigantea_Tropicos_100014590.jpg
		+	\| companyimage2=TropicosLogo.gif
		+	\| companyURL2=http://www.tropicos.org/Image/100014590
		+	\| reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/100014590>
		+
		+	\| }}
		+
		+
	=Organoleptic Characteristics=		=Organoleptic Characteristics=

@@ Line 1: / Line 1: @@
 =Nomenclature=
@@ Line 11: / Line 12: @@
 |notes= }}
-=Macroscopic Entries=
+=Botanical Voucher Specimen=
-[[Category:NoMacroscopy]]
+=Organoleptic Characteristics=
-=Microscopic Entries=
+=Macroscopic Characteristics=
-[[Category:NoMicroscopy]]
+=Microscopic Characteristics=
-=HPTLC Entries=
+=High Performance Thin Layer Chromatographic Identification=
 {{HPTLC | source=HPTLC Association
               | companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
@@ Line 59: / Line 60: @@
               | }}
-=Other Points of Interest=
+=Supplementary Information=
+=Sources=
-[[Category:Blank2]]
+<references />