Terminalia bellerica (fruit) - Revision history

Admin: add alkemist labs microscopy

2016-04-08T15:44:25Z

add alkemist labs microscopy

Admin: add askbox

2014-06-02T21:24:14Z

add askbox

Staffer at 02:57, 16 March 2014

2014-03-16T02:57:43Z

Staffer: Various title corrections, Source additions

2014-03-16T02:46:50Z

Various title corrections, Source additions

Staffer: Nomenclature updated

2014-03-14T22:02:12Z

Nomenclature updated

AHPAStaff: addition of hptlc info.--jd

2013-06-25T00:10:43Z

addition of hptlc info.--jd

New page

=Introduction=
[[Category:NoIntro]]

=Macroscopic Entries=
[[Category:NoMacroscopy]]

=Microscopic Entries=
[[Category:NoMicroscopy]]

=HPTLC Entries=
{{HPTLC | source=HPTLC Association
| companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
| companyURL=http://www.hptlc-association.org/
| mainimage=Terminalia bellerica-UV 254 nm-hptlc-association.png
| caption1=Belleric myrobalan fruit, mao he zi (fruit) HPTLC ID - UV 254 nm
| description=Belleric myrobalan fruit, mao he zi (''Terminalia bellerica'')
| image2=Terminalia bellerica-UV 366 nm, after derivatization-hptlc-association.png
| caption2=Belleric myrobalan fruit, mao he zi (fruit) HPTLC ID - UV 366 nm, after derivatization
|
| stationaryphase=Stationary phase, i.e. Silica gel 60, F254
| mobilephase=Ethyl formate, toluene, formic acid, water 30:1.5:4:3 (v/v/v/v)
| prep=Sample: Mix 1.0 g of powdered sample with 10 mL of methanol and sonicate for 10 minutes, then centrifuge or filter and use the supernatants/filtrates as test solutions.

Derivatization reagent: 1.) NP reagent, Preparation: 1 g of natural products reagent in 200 mL of ethyl acetate; 2.) PEG reagent, Preparation: 10 g of polyethylene glycol 400 in 200 mL of dichloromethane, Use: Heat plate for 3 min at 100°C, dip (time 0, speed 5) in NP reagent, dry, dip (time 0, speed 5) in PEG reagent.
| detection=Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33%
| referencesamples=Reference: Dissolve 1 mg of chebulinic acid and 2 mg gallic acid in 10 mL of methanol. Optional: dissolve 1 mg of ellagic acid in 10 mL of methanol.
|
| lanes=Lanes, from left to right (Track, Volume, Sample):
# 5 µL Chebulinic acid, ellagic acid, and gallic acid (increasing Rf)
# '''1 µL Belleric myrobalan fruit'''
# 1 µL Chebulic myrobalan fruit
# 1 µL Amla fruit
# 1 µL Triphala powder

| notes=''Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.''

System suitability test: Chebulinic acid: a quenching zone at Rf ~ 0.26 (UV 245 nm). Gallic acid: a quenching zone at Rf ~ 0.71 (UV 245 nm).

Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. Under UV 254 nm the test solution shows several quenching zones between Rf ~ 0.18 and 0.28. A characteristic well defined quenching zone at Rf ~ 0.53 is present (red arrow). At the position of gallic acid a faint dark zone is visible. Under UV 366 nm there is a blue fluorescent zone at Rf ~ 0.21 and a characteristic fluorescent zone at Rf ~ 0.53 (green arrow).

Test for other species: Under UV 366 nm there are no fluorescent zones between Rf ~ 0.40 and 0.52 and below Rf ~ 0.14 (Chebulic myrobalan fruit, Amla fruit, pink arrows).

| }}

{{HPTLC | source=HPTLC Association
| companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
| companyURL=http://www.hptlc-association.org/
| mainimage=Triphala powder-UV 254 nm-hptlc-association.png
| caption1=Triphala powder (mixture) HPTLC ID - UV 254 nm
| description=Triphala powder (mixture of ''Phyllanthus emblica, Terminalia bellerica, Terminalia chebula'')
| image2=Triphala powder-UV 366 nm, after derivatization-hptlc-association.png
| caption2=Triphala powder(mixture) HPTLC ID - UV 366 nm, after derivatization
|
| stationaryphase=Stationary phase, i.e. Silica gel 60, F254
| mobilephase=Ethyl formate, toluene, formic acid, water 30:1.5:4:3 (v/v/v/v)
| prep=Sample: Mix 1.0 g of powdered sample with 10 mL of methanol and sonicate for 10 minutes, then centrifuge or filter and use the supernatants/filtrates as test solutions.

Derivatization reagent: 1.) NP reagent, Preparation: 1 g of NP reagent in 200 mL of ethyl acetate; 2.) PEG reagent, Preparation: 10 g of polyethylene glycol 400 in 200 mL of dichloromethane, Use: Heat plate 3min at 100°C, dip (time 0, speed 5) in NP reagent, dry and dip (time 0, speed 5) in PEG reagent.
| detection=Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33%
| referencesamples=Reference: Dissolve 1 mg of chebulinic acid and 2 mg of gallic acid in 10 mL of methanol. Optional: dissolve 1 mg of ellagic acid in 10 mL of methanol.
|
| lanes=Lanes, from left to right (Track, Volume, Sample):
# 5 µL Chebulinic acid, ellagic acid, and gallic acid (increasing Rf)
# 1 µL Belleric myrobalan fruit
# 1 µL Chebulic myrobalan fruit
# 1 µL Amla fruit
# '''1 µL Triphala powder 1'''
# '''1 µL Triphala powder 2'''
# '''1 µL Triphala powder 3'''
# '''1 µL Triphala powder 4'''
# 1 µL Triphala tablets 1
# 1 µL Triphala tablets 2
# 1 µL Triphala tablets 3
# 1 µL Triphala tablets 4
# 1 µL Triphala tablets 5
# 2 µL Triphala powder extract
# 2 µL Triphala tablets 6

| notes=''Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.''

System suitability test: Chebulinic acid: a quenching zone at Rf ~ 0.26 (UV 245 nm). Gallic acid: a quenching zone at Rf ~ 0.71 (UV 245 nm).

Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. Under UV 254 nm the test solution shows several diffuse quenching zones above the application position. The two main quenching zones are detected at Rf ~ 0.19 and Rf ~ 0.28 (red arrows). Above these zones there are additional diffuse quenching zones. There are quenching zones at the position of ellagic acid and of gallic acid. Under UV 366 nm two blue fluorescent zones are detected right above the application position (yellow arrow). Several intense blue fluorescent zones are present between Rf ~ 0.16 and 0.40. At the position of gallic acid there is a fluorescent zone.
| }}

=Other Points of Interest=

[[Category:Blank2]]

@@ Line 17: / Line 17: @@
 =Macroscopic Characteristics=
 =Microscopic Characteristics=
 =High Performance Thin Layer Chromatographic Identification=

← Older revision		Revision as of 21:24, 2 June 2014
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−	{{DISPLAYTITLE:''Terminalia bellerica'' (fruit) }}	+	{{DISPLAYTITLE:''Terminalia bellerica'' (fruit) }} {{askbox\|herb=''Terminalia bellerica''}}
	=Nomenclature=		=Nomenclature=

← Older revision		Revision as of 02:57, 16 March 2014
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−	{{DISPLAYTITLE:''~~terminalia~~ bellerica'' (fruit) }}	+	{{DISPLAYTITLE:''Terminalia bellerica'' (fruit) }}
	=Nomenclature=		=Nomenclature=

@@ Line 1: / Line 1: @@
 =Nomenclature=
@@ Line 11: / Line 12: @@
 |notes= }}
-=Macroscopic Entries=
+=Botanical Voucher Specimen=
-[[Category:NoMacroscopy]]
+=Organoleptic Characteristics=
-=Microscopic Entries=
+=Macroscopic Characteristics=
-[[Category:NoMicroscopy]]
+=Microscopic Characteristics=
-=HPTLC Entries=
+=High Performance Thin Layer Chromatographic Identification=
 {{HPTLC | source=HPTLC Association
               | companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
@@ Line 95: / Line 96: @@
               | }}
-=Other Points of Interest=
+=Supplementary Information=
+=Sources=
-[[Category:Blank2]]
+<references />