Verbascum spp. (flower) - Revision history

Admin: add macro characteristics

2015-06-24T16:16:16Z

add macro characteristics

Admin: add PlantaPhile references

2015-04-28T16:07:15Z

add PlantaPhile references

Admin: add USD 1918 information (organoleptic and macroscopic)

2015-04-28T14:33:36Z

add USD 1918 information (organoleptic and macroscopic)

Admin: add askbox

2014-06-02T21:29:25Z

add askbox

Admin at 13:07, 27 March 2014

2014-03-27T13:07:26Z

Staffer: Vouchers added

2014-03-27T03:33:27Z

Vouchers added

Staffer: Various title corrections, Source additions

2014-03-16T03:04:50Z

Various title corrections, Source additions

AHPAStaff: addition of hptlc info.--jd

2013-06-25T23:45:47Z

addition of hptlc info.--jd

New page

=Introduction=
[[Category:NoIntro]]

=Macroscopic Entries=
[[Category:NoMacroscopy]]

=Microscopic Entries=
[[Category:NoMicroscopy]]

=HPTLC Entries=
{{HPTLC | source=HPTLC Association
| companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
| companyURL=http://www.hptlc-association.org/
| mainimage=Verbascum spp-hptclc-association.png
| caption1=Mullein (flower) HPTLC ID - NP and PEG reagent, UV 366 nm
| description=Mullein (flower) (''Verbascum thapsus'' L., ''V. densiflorum Bertol''. syn. ''V. thapsiforme'' Schrad, ''V. phlomoides'' L.)
|
| stationaryphase=Stationary phase, i.e. Silica gel 60, F254
| mobilephase=Ethyl acetate, formic acid, water, ethyl methyl ketone 50:10:10:30 (v/v/v/v)
| prep=Sample: Mix 1 g of powdered sample with 10 mL of methanol and sonicate at 60°C for 10 minutes, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions.

Derivatization reagent: 1.) NP reagent, Preparation: 1 g of NP reagent in 200 mL ethyl acetate; 2.) PEG reagent, Preparation: 10g of polyethylene glycol 400 in 200 mL of dichloromethane, Use: Heat plate for 3 min at 100°C, then dip (time 0, speed 5) in NP reagent, dry and dip (time 0, speed 5) in PEG reagent.
| detection=Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33%
| referencesamples=Reference: Dissolve 3.5 mg of rutin in 5 mL of methanol; Dissolve 2 mg of caffeic acid in 5 mL of methanol; Optional: 4 mg of hyperoside in 5 mL of methanol.
|
| lanes=Lanes, from left to right (Track, Volume, Sample):
# 2 µL Mullein flower 1
# '''4 µL Mullein flower 1'''
# 8 µL Mullein flower 1
# 1 µL Mullein flower 1 (Ph. Eur extr.)
# 3 µL Mullein flower 1 (Ph. Eur extr.)
# 6 µL Mullein flower 1 (Ph. Eur extr.)
# 2 µL Caffeic acid
# 2 µL Rutin
# 2 µL Hyperoside
# 2 µL Mullein flower 2
# 4 µL Mullein flower 2
# 8 µL Mullein flower 2
# 2 µL Mullein flower 3
# 4 µL Mullein flower 3
# 8 µL Mullein flower 3

| notes=''Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.''

System suitability test: Rutin: orange fluorescent zone at Rf ~ 0.38; Caffeic acid: blue fluorescent zone at Rf ~ 0.89.

Identification: Compare result with reference images in Image Comparison Viewer. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows an intense bluish zone at Rf ~ 0.40 just above the position of reference substance rutin. There is another bluish zone at Rf ~ 0.60 slightly higher than the position of reference substance hyperoside. Just below the position of reference substance caffeic acid there is an intense bluish zone at Rf ~ 0.82 and just above caffeic acid there is a yellow zone at Rf ~ 0.90.

| }}

=Other Points of Interest=

[[Category:Blank2]]

@@ Line 40: / Line 40: @@
 {| border=1
+|
 {{Macroscopy | source=United States Dispensatory (1918)
 | description=Corolla light-yellow, the outer surface grayish with a fine, soft, woolly indumentum, the inner surface sparsely hairy and finely veined; tube of the corolla only 1 or 2 mm. in length and almost equally broad, the limb from 14 to 30 mm. in breadth, between wheel-shaped and saucer-shaped, obscurely two-lipped, the unequal lobes rounded-obovate. Stamens five, borne on the base of the corolla, shorter than the corolla, two of them longer than the other tliree, the filaments thick and fleshy, more or less pilose, especially the three shorter ones, usually orange colored. Stamen-hairs cylindrical, unicellular, non-branching, surface minutely reticulate, apex rounded, frequently enlarged. Pollen grains smooth, triangular and more or less rounded, from 0.025 to 0.03 mm. in diameter. The flowers impart a yellow color to boiling water, and a rather permanent green color with dilute sulphuric acid, the latter color becoming brown upon the addition of alkalies.

← Older revision		Revision as of 16:07, 28 April 2015
Line 44:		Line 44:

	Obovate with narrowed base, or varying to oblong or oblong-lanceolate, without a true petiole, obtuse or tending toward acuteness at the summit, from 1 to 6 dm. in length and from 3 to 15 cm. in breadth; very thick, rather tough, light yellowish-gray or greenish-gray, densely long-tomentose, with numerous, multicellular, branching, non-glandular hairs.}}		Obovate with narrowed base, or varying to oblong or oblong-lanceolate, without a true petiole, obtuse or tending toward acuteness at the summit, from 1 to 6 dm. in length and from 3 to 15 cm. in breadth; very thick, rather tough, light yellowish-gray or greenish-gray, densely long-tomentose, with numerous, multicellular, branching, non-glandular hairs.}}
		+	{{ Media2 \| cat=Macroscopy
		+	\| source=PlantaPhile
		+	\| mainimage=PlantaPhile - 301.jpg
		+	\| companyimage=PlantaPhile logo.jpg
		+	\| companyURL=http://plantaphile.com/
		+
		+	\| source2=PlantaPhile
		+	\| image2=PlantaPhile - 2602.jpg
		+	\| companyimage2=PlantaPhile logo.jpg
		+	\| companyURL2=http://plantaphile.com/
		+	\| }}
	\|}		\|}

@@ Line 30: / Line 30: @@
 =Organoleptic Characteristics=
 =Macroscopic Characteristics=
 =Microscopic Characteristics=

← Older revision		Revision as of 21:29, 2 June 2014
Line 1:		Line 1:
−	{{DISPLAYTITLE:''Verbascum'' spp. (flower) }}	+	{{DISPLAYTITLE:''Verbascum'' spp. (flower) }} {{askbox\|herb=''Verbascum'' spp.}}
	=Nomenclature=		=Nomenclature=

@@ Line 10: / Line 10: @@
               | companyimage=TropicosLogo.gif
               | companyURL=http://www.tropicos.org/Image/100123204
-              | reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/100123204>
+              | reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014
-              | caption1="Verbascum thapsus"
+              | caption1=''Verbascum thapsus''
               | source2=MOBOT, Tropicos.org
@@ Line 17: / Line 17: @@
               | companyimage2=TropicosLogo.gif
               | companyURL2=http://www.tropicos.org/Image/100279961
-              | reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/100279961>
+              | reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014
-              | caption2="Verbascum densiflorum"
+              | caption2=''Verbascum densiflorum''
               | source3=MOBOT, Tropicos.org
@@ Line 24: / Line 24: @@
               | companyimage3=TropicosLogo.gif
               | companyURL3=http://www.tropicos.org/Image/100123203
-              | reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/100123203>
+              | reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014
-              | caption3="Verbascum phlomoides"
+              | caption3=''Verbascum phlomoides''
               | }}

← Older revision		Revision as of 03:33, 27 March 2014
Line 3:		Line 3:

	=Botanical Voucher Specimen=		=Botanical Voucher Specimen=
		+
		+	{{Media3 \| cat=Voucher
		+
		+	\| source=MOBOT, Tropicos.org
		+	\| mainimage=Verbascum_thapsus_Tropicos_100123204_(S).jpg
		+	\| companyimage=TropicosLogo.gif
		+	\| companyURL=http://www.tropicos.org/Image/100123204
		+	\| reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/100123204>
		+	\| caption1="Verbascum thapsus"
		+
		+	\| source2=MOBOT, Tropicos.org
		+	\| image2=Verbascum_densiflorum_Tropicos_100279961_(S).jpg
		+	\| companyimage2=TropicosLogo.gif
		+	\| companyURL2=http://www.tropicos.org/Image/100279961
		+	\| reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/100279961>
		+	\| caption2="Verbascum densiflorum"
		+
		+	\| source3=MOBOT, Tropicos.org
		+	\| image3=Verbascum_phlomoides_Tropicos_100123203_(S).jpg
		+	\| companyimage3=TropicosLogo.gif
		+	\| companyURL3=http://www.tropicos.org/Image/100123203
		+	\| reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/100123203>
		+	\| caption3="Verbascum phlomoides"
		+
		+	\| }}
		+
	=Organoleptic Characteristics=		=Organoleptic Characteristics=

@@ Line 1: / Line 1: @@
-=Introduction=
+{{DISPLAYTITLE:''Verbascum'' spp. (flower) }}
-[[Category:NoIntro]]
+=Nomenclature=
-=Macroscopic Entries=
+=Botanical Voucher Specimen=
-[[Category:NoMacroscopy]]
+=Organoleptic Characteristics=
-=Microscopic Entries=
+=Macroscopic Characteristics=
-[[Category:NoMicroscopy]]
+=Microscopic Characteristics=
-=HPTLC Entries=
+=High Performance Thin Layer Chromatographic Identification=
 {{HPTLC | source=HPTLC Association
               | companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
@@ Line 50: / Line 50: @@
-=Other Points of Interest=
+=Supplementary Information=
+=Sources=
-[[Category:Blank2]]
+<references />