Viola spp. (aerial parts) - Revision history

Admin: add Culbreth ref for organoleptic, macro

2015-07-07T18:55:49Z

add Culbreth ref for organoleptic, macro

Admin: add askbox

2014-06-02T21:29:50Z

add askbox

Admin at 13:06, 27 March 2014

2014-03-27T13:06:43Z

Staffer: Vouchers added

2014-03-27T03:40:38Z

Vouchers added

Staffer: Various title corrections, Source additions

2014-03-16T03:07:38Z

Various title corrections, Source additions

AHPAStaff: addition of hptlc info.--jd

2013-06-26T00:13:20Z

addition of hptlc info.--jd

New page

=Introduction=
[[Category:NoIntro]]

=Macroscopic Entries=
[[Category:NoMacroscopy]]

=Microscopic Entries=
[[Category:NoMicroscopy]]

=HPTLC Entries=
{{HPTLC | source=HPTLC Association
| companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
| companyURL=http://www.hptlc-association.org/
| mainimage=Viola spp-hptlc-association.png
| caption1=Wild pansy flowering (aerial parts) HPTLC ID - NP and PEG reagent, UV 366 nm
| description=Wild pansy flowering (aerial parts) (''Viola arvensis, V. tricolor'')
|
| stationaryphase=Stationary phase, i.e. Silica gel 60, F254
| mobilephase=Formic acid, acetic acid, water, ethyl acetate 11:11:27:100 (v/v/v/v)
| prep=Sample: Mix 2 g of powdered sample with 10 mL of methanol and sonicate for 10 minutes, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions.

Derivatization reagent: 1.) NP reagent, Preparation: 1 g of natural products reagent in 200 mL of ethyl acetate; 2.) PEG reagent, Preparation: 10 g of polyethylene glycol 400 in 200 mL of dichloromethane, Use: Heat plate for 3 min at 100°C, dip (time 0, speed 5) in NP reagent, dry and dip (time 0, speed 5) in PEG reagent
| detection=Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33%
| referencesamples=Reference: Individually dissolve 2.5 mg of rutin, 1.0 mg of caffeic acid, and 2.5 mg of hyperoside each in 10 mL of methanol.
|
| lanes=Lanes, from left to right (Track, Volume, Sample):
# 2 µL Wild pansy 1
# 4 µL Wild pansy 1
# '''8 µL Wild pansy 1'''
# 1 µL Wild pansy 1 (Extraction Ph. Eur)
# 2 µL Wild pansy 1 (Extraction Ph. Eur)
# 3 µL Wild pansy 1 (Extraction Ph. Eur)
# 2 µL Rutin
# 2 µL Hyperoside
# 2 µL Caffeic acid
# 2 µL Wild pansy 2
# 4 µL Wild pansy 2
# 8 µL Wild pansy 2

| notes=''Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.''

System suitability test: Caffeic acid: blue fluorescent zone at Rf ~ 0.87. Rutin: orange fluorescent zone at Rf ~ 0.32.

Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows a blue fluorescent zone just below the zone due to reference caffeic acid (orange arrow). A yellow fluorescent zone is seen at Rf ~ 0.40 below the position of reference hyperoside (yellow arrow). Below it there is an intense yellowish orange fluorescent zone corresponding to reference rutin. Below the zone due to rutin there are three yellowish-green fluorescent zones (red arrow).

| }}

=Other Points of Interest=

[[Category:Blank2]]

@@ Line 24: / Line 24: @@
 =Organoleptic Characteristics=
+{| border=1
 =Macroscopic Characteristics=
 =Microscopic Characteristics=

← Older revision		Revision as of 21:29, 2 June 2014
Line 1:		Line 1:
−	{{DISPLAYTITLE:''Viola'' spp. (aerial parts) }}	+	{{DISPLAYTITLE:''Viola'' spp. (aerial parts) }} {{askbox\|herb=''Viola'' spp.}}
	=Nomenclature=		=Nomenclature=

@@ Line 11: / Line 11: @@
               | companyURL=http://www.tropicos.org/Image/100123424
               | reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/100123424>
-              | caption1="Viola arvensis"
+              | caption1=''Viola arvensis''
               | source2=MOBOT, Tropicos.org
@@ Line 18: / Line 18: @@
               | companyURL2=http://www.tropicos.org/Image/100000826
               | reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/100000826>
-              | caption2="Viola tricolor"
+              | caption2=''Viola tricolor''
               | }}

← Older revision		Revision as of 03:40, 27 March 2014
Line 3:		Line 3:

	=Botanical Voucher Specimen=		=Botanical Voucher Specimen=
		+
		+	{{Media2 \| cat=Voucher
		+
		+	\| source=MOBOT, Tropicos.org
		+	\| mainimage=Viola_arvensis_Tropicos_100123424_(S).jpg
		+	\| companyimage=TropicosLogo.gif
		+	\| companyURL=http://www.tropicos.org/Image/100123424
		+	\| reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/100123424>
		+	\| caption1="Viola arvensis"
		+
		+	\| source2=MOBOT, Tropicos.org
		+	\| image2=Viola_tricolor_Tropicos_100000826_(S).jpg
		+	\| companyimage2=TropicosLogo.gif
		+	\| companyURL2=http://www.tropicos.org/Image/100000826
		+	\| reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/100000826>
		+	\| caption2="Viola tricolor"
		+
		+	\| }}
		+
		+
	=Organoleptic Characteristics=		=Organoleptic Characteristics=

@@ Line 1: / Line 1: @@
-=Introduction=
+{{DISPLAYTITLE:''Viola'' spp. (aerial parts) }}
-[[Category:NoIntro]]
+=Nomenclature=
-=Macroscopic Entries=
+=Botanical Voucher Specimen=
-[[Category:NoMacroscopy]]
+=Organoleptic Characteristics=
-=Microscopic Entries=
+=Macroscopic Characteristics=
-[[Category:NoMicroscopy]]
+=Microscopic Characteristics=
-=HPTLC Entries=
+=High Performance Thin Layer Chromatographic Identification=
 {{HPTLC | source=HPTLC Association
               | companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
@@ Line 47: / Line 47: @@
-=Other Points of Interest=
+=Supplementary Information=
+=Sources=
-[[Category:Blank2]]
+<references />