Euphrasia stricta (aerial parts)

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=Introduction=
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{{DISPLAYTITLE:''Euphrasia stricta'' (aerial parts) }} {{askbox|herb=''Euphrasia stricta''}}
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=Macroscopic Entries=
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=Nomenclature=
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=Microscopic Entries=
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{{nomenclature | binomial=Euphrasia stricta
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=HPTLC Entries=
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|authority=J.P. Wolff ex J.F. Lehm.
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=Other Points of Interest=
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|family=Scrophulariaceae
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[[Category:blank]]
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|scn=eyebright
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|syn=''Euphrasia officinalis''
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|ayurvedic=
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|pinyin=
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|aka=
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|notes=}}
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=Botanical Voucher Specimen=
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{{Media2      |cat=Voucher
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            | source=MOBOT, Tropicos.org
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            | mainimage=Euphrasia officinalis Tropicos 100285389.jpg
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            | companyimage=TropicosLogo.gif
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            | companyURL=http://www.tropicos.org/Image/100285389
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            | reference=Tropicos.org. Missouri Botanical Garden. 19 Mar 2014 <http://www.tropicos.org/Image/100285389>
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| companyimage2=Kewlogo.gif
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| companyURL2=http://specimens.kew.org/herbarium/K000195686
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| image2=Euphrasia_officinalis_Kew_imageBarcode=K000195686_98404.jpg
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| source2=Royal Botanic Gardens, Kew.
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            | }}
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=Organoleptic Characteristics=
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{| border=1
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|
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{{Organolepsy | source=United States Dispensatory (1918)
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| description=''E. officinalis'' is a small annual plant without odor, and of a bitterish, astringent taste.}}
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|}
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=Macroscopic Characteristics=
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{{ Media2    | cat=Macroscopy
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            | source=PlantaPhile
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            | mainimage=PlantaPhile - 3012.jpg
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            | companyimage=PlantaPhile logo.jpg
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            | companyURL=http://plantaphile.com/
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            | source2=PlantaPhile
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            | image2=PlantaPhile - 2644.jpg
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            | companyimage2=PlantaPhile logo.jpg
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            | companyURL2=http://plantaphile.com/
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            | }}
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=Microscopic Characteristics=
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=High Performance Thin Layer Chromatographic Identification=
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{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
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            | description=Eyebright (aerial parts) (''Euphrasia officinalis'')
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            | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL=http://www.alkemist.com
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            | mainimage=Euphrasia_officinalis_-_Alkemists_Laboratories.jpg
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            | caption1=''Euphrasia officinalis'' HPTLC ID - Vanillin/Sulfuric Acid Reagent UV 365 nm
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            | stationaryphase=Silica gel 60, F254, 10 x 10 cm HPTLC plates
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            | mobilephase=chloroform: methanol: water [6/4/0.4]
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            | prep=0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr
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            | detection=Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm
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            | lanes= Lanes, from left to right (Track, Volume, Sample):
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# 2 μL Aucubin ~0.1% in CH3OH
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# 3 μL ''Euphrasia officinalis''-1 (aerial parts)
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# 3 μL ''Euphrasia officinalis''-2 (aerial parts)
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# 3 μL ''Euphrasia officinalis''-3 (aerial parts)
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# 3 μL ''Euphrasia officinalis''-3 (aerial parts)
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# 3 μL ''Euphrasia officinalis''-4 (aerial parts)
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# 3 μL ''Euphrasia officinalis''-5 (aerial parts)
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# 2 μL Catalpol ~0.1% in CH3OH
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Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.
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            | reference=Herbal Drugs and Phytopharmaceuticals, Max Wichtl, 3rd ed., 2004
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            | }}
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=Supplementary Information=
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=Sources=
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<references />

Latest revision as of 18:57, 19 July 2016

AHPA recognizes other valuable resources exist regarding the identity of Euphrasia stricta.

To submit a suggestion or contribution, please contact Merle Zimmermann.

Contents

Nomenclature

Euphrasia stricta J.P. Wolff ex J.F. Lehm.   Scrophulariaceae  
Syn. Euphrasia officinalis  
Standardized common name (English): eyebright

Botanical Voucher Specimen

bottomright bottomright

Euphrasia officinalis Tropicos 100285389.jpg
Source: MOBOT, Tropicos.org[1]

Euphrasia officinalis Kew imageBarcode=K000195686 98404.jpg
Source: Royal Botanic Gardens, Kew.[2]


Organoleptic Characteristics

E. officinalis is a small annual plant without odor, and of a bitterish, astringent taste.

Source: United States Dispensatory (1918) [3]

Macroscopic Characteristics

bottomright bottomright

PlantaPhile - 3012.jpg
Source: PlantaPhile[4]

PlantaPhile - 2644.jpg
Source: PlantaPhile[5]

Microscopic Characteristics

High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Euphrasia officinalis HPTLC ID - Vanillin/Sulfuric Acid Reagent UV 365 nm

Eyebright (aerial parts) (Euphrasia officinalis)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 2 μL Aucubin ~0.1% in CH3OH
  2. 3 μL Euphrasia officinalis-1 (aerial parts)
  3. 3 μL Euphrasia officinalis-2 (aerial parts)
  4. 3 μL Euphrasia officinalis-3 (aerial parts)
  5. 3 μL Euphrasia officinalis-3 (aerial parts)
  6. 3 μL Euphrasia officinalis-4 (aerial parts)
  7. 3 μL Euphrasia officinalis-5 (aerial parts)
  8. 2 μL Catalpol ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase chloroform: methanol: water [6/4/0.4] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see Herbal Drugs and Phytopharmaceuticals, Max Wichtl, 3rd ed., 2004


Source: Elan M. Sudberg, Alkemist Laboratories [6]


Supplementary Information

Sources

  1. ↑ MOBOT, Tropicos.org http://www.tropicos.org/Image/100285389
  2. ↑ Royal Botanic Gardens, Kew. http://specimens.kew.org/herbarium/K000195686
  3. ↑ United States Dispensatory (1918)
  4. ↑ PlantaPhile http://plantaphile.com/
  5. ↑ PlantaPhile http://plantaphile.com/
  6. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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