Rehmannia glutinosa (root)

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=HPTLC Entries=
 
=HPTLC Entries=
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{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
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            | description=Rehmannia (root) (''Rehmannia glutinosa'')
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            | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL=http://www.alkemist.com
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            | mainimage=Rehmannia glutinosa root - Alkemists Laboratories.jpg
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            | caption1=''Rehmannia glutinosa'' HPTLC ID - Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm
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            | stationaryphase=Silica gel 60, F254, 10 x 10 cm HPTLC plates
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            | mobilephase=chloroform: methanol: water [7/3/0.5]
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            | prep=0.3 g + 3 ml CH3OH sonicate/heat @ 50° C ~ 1/2 hr
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            | detection=Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm
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            | lanes= Lanes, from left to right (Track, Volume, Sample):
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# 3 μL ''Rehmannia glutinosa''-1 (root)
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# 3 μL ''Rehmannia glutinosa''-2 (root)
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# 3 μL ''Rehmannia glutinosa''-3 (root)
 +
# 3 μL ''Rehmannia glutinosa''-4 (root)
 +
# 3 μL ''Rehmannia glutinosa''-4 (root)
 +
# 3 μL ''Rehmannia glutinosa''-5 (root)
 +
# 3 μL ''Rehmannia glutinosa''-6 (root)
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# 2 μL Catalpol ~ 0.1% in CH3OH
 +
 +
Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.
 +
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            | reference=Japanese Official Monographs part III
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            | }}
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=Other Points of Interest=
 
=Other Points of Interest=
 
[[Category:NoIntro]]
 
[[Category:NoIntro]]

Revision as of 22:04, 15 March 2013

Contents

Introduction

Macroscopic Entries

Microscopic Entries

Parenchyma showing red pigmented material observed at 400x with Acidified Chloral Hydrate Glycerol Solution.

Source: Elan M. Sudberg, Alkemist Laboratories [1]

AP-LOGO-Laboratories Crop - Copy.jpg
Rehmannia glutinosa - Alkemist Laboratories.jpg


HPTLC Entries

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Rehmannia glutinosa HPTLC ID - Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm

Rehmannia (root) (Rehmannia glutinosa)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Rehmannia glutinosa-1 (root)
  2. 3 μL Rehmannia glutinosa-2 (root)
  3. 3 μL Rehmannia glutinosa-3 (root)
  4. 3 μL Rehmannia glutinosa-4 (root)
  5. 3 μL Rehmannia glutinosa-4 (root)
  6. 3 μL Rehmannia glutinosa-5 (root)
  7. 3 μL Rehmannia glutinosa-6 (root)
  8. 2 μL Catalpol ~ 0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase chloroform: methanol: water [7/3/0.5] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicate/heat @ 50° C ~ 1/2 hr 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see Japanese Official Monographs part III


Source: Elan M. Sudberg, Alkemist Laboratories [2]

Other Points of Interest


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