Fragment of a unicellular thick walled trichome seen under polarized light observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories^[1]

Rosette of calcium oxalate observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories^[2]

Rosa canina HPTLC ID - Vanillin/Sulfuric Acid Visible Light

Rosehips (fruit) (Rosa canina)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

1. 5 μL Ascorbic Acid ~0.1% in CH3OH
2. 3 μL Rosa canina-1 (fruit)
3. 5 μL Rosa canina-1 (fruit)
4. 4 μL Rosa canina-2 (fruit)
5. 4 μL Rosa canina-2 (fruit)
6. 4 μL Rosa canina-3 (fruit)
7. 4 μL Rosa canina-4 (fruit)
8. 5 μL Ascorbic Acid ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase toluene: acetone: HCOOH [6/3/1] 

Sample Preparation Method 0.3g+3mL 70% grain EtOH sonicate/heat @~50° C ~ 1/2 hr 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> visible light 

Reference see Adapted from European Pharmacopoeia 5.0 2005

Source: Elan M. Sudberg, Alkemist Laboratories ^[3]