Vaccinium macrocarpon HPTLC ID - 10% Ethanolic Sulfuric Acid 365 nm

Cranberry (fruit) (Vaccinium macrocarpon)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

1. 3 μL Catechin ~0.1% in CH3OH
2. 3 μL Vaccinium macrocarpon-1 (fruit)
3. 3 μL Vaccinium macrocarpon-2 (fruit)
4. 3 μL Vaccinium macrocarpon-3 (fruit)
5. 3 μL Vaccinium macrocarpon-3 (fruit)
6. 4 μL Vaccinium macrocarpon-4 (fruit)
7. 3 μL Vaccinium macrocarpon-4 (fruit)
8. 3 μL Gallic Acid ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase CHCl3: CH3OH: HCOOH: H2O [6/2/1.4/0.1] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicate/heat @ 50° C ~ 1/2 hr 

Detection Method 10% Ethanolic Sulfuric Acid -> 115° C 15 min -> UV 365 nm 

Reference see American Herbal Pharmacopoeia & Therapeutic Compendium

Source: Elan M. Sudberg, Alkemist Laboratories ^[2]