Hibiscus sabdariffa (flower)
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| mainimage=Hibiscus Alkemist Laboratories.jpg | | mainimage=Hibiscus Alkemist Laboratories.jpg | ||
| caption1=Rosettes of Calcium oxalate with pink pigment observed at 400x with Acidified Chloral Hydrate Glycerol Solution. | | caption1=Rosettes of Calcium oxalate with pink pigment observed at 400x with Acidified Chloral Hydrate Glycerol Solution. | ||
| − | | description= | + | | description= Hibiscus (flower) (''Hibiscus sabdariffa'') |
| image2=Hibiscus-1 Alkemist Laboratories.jpg | | image2=Hibiscus-1 Alkemist Laboratories.jpg | ||
| caption2=Fragment of a thin walled trichome observed at 400x with Acidified Chloral Hydrate Glycerol Solution. | | caption2=Fragment of a thin walled trichome observed at 400x with Acidified Chloral Hydrate Glycerol Solution. | ||
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=HPTLC Entries= | =HPTLC Entries= | ||
| + | {{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories | ||
| + | | description=Hibiscus (flower) (''Hibiscus sabdariffa'') | ||
| + | | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg | ||
| + | | companyURL=http://www.alkemist.com | ||
| + | | mainimage=Hibiscus_sabdariffa_-_Alkemists_Laboratories.jpg | ||
| + | | caption1=''Hibiscus sabdariffa'' HPTLC ID - Thymol Sulfuric Acid Reagent UV 365 nm | ||
| + | | stationaryphase=Silica gel 60, F254, 10 x 10 cm HPTLC plates | ||
| + | | mobilephase=tolulene: ethyl formate: formic acid [5/4/1] | ||
| + | | prep=0.5 g + 5mL 70%gr EtOH sonicate/heat @ 50° C~1/2 hr, evap w N2, qs 1mL CH3OH | ||
| + | | detection=Thymol Sulfuric acid spray -> 115° C 10 min -> UV 365 nm | ||
| + | | lanes= Lanes, from left to right (Track, Volume, Sample): | ||
| + | # μL Epicatechin ~0.1% in CH3OH | ||
| + | # μL ''Hibiscus sabdariffa''-1 (flower) | ||
| + | # μL ''Hibiscus sabdariffa''-2 (flower) | ||
| + | # μL ''Hibiscus sabdariffa''-3 (flower) | ||
| + | # μL ''Hibiscus sabdariffa''-3 (flower) | ||
| + | # μL ''Hibiscus sabdariffa''-4 (flower) | ||
| + | # μL ''Hibiscus sabdariffa''-5 (flower) | ||
| + | # μL Catechin ~0.1% in CH3OH | ||
| + | |||
| + | Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA. | ||
| + | |||
| + | | reference=Thin-Layer Chromatography Jork, Funk Wimmer P421-424 | ||
| + | | }} | ||
| + | |||
| + | |||
=Other Points of Interest= | =Other Points of Interest= | ||
[[Category:blank]] | [[Category:blank]] | ||
Revision as of 22:49, 22 October 2012
Contents |
Introduction
Macroscopic Entries
Microscopic Entries
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HPTLC Entries
 
Hibiscus (flower) (Hibiscus sabdariffa) Lane Assignments Lanes, from left to right (Track, Volume, Sample):
Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA. Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates Mobile Phase tolulene: ethyl formate: formic acid [5/4/1] Sample Preparation Method 0.5 g + 5mL 70%gr EtOH sonicate/heat @ 50° C~1/2 hr, evap w N2, qs 1mL CH3OH Detection Method Thymol Sulfuric acid spray -> 115° C 10 min -> UV 365 nm Reference see Thin-Layer Chromatography Jork, Funk Wimmer P421-424
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Other Points of Interest
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