Piper nigrum (fruit)

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=Introduction=
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{{DISPLAYTITLE:''Achillea millefolium'' (flower)}}
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{{Microscopy | source=Elan M. Sudberg, Alkemist Laboratories
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=Botanical Voucher Specimen=
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=Organoleptic Characteristics=
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=Macroscopic Descriptions=
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{{Microscopy | source= Elan M. Sudberg, Alkemist Laboratories
 
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      | mainimage=Organic Black Pepper Alkemist Laboratories.jpg
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       | description= Cellular structures identified in this botanical specimen are yellow oil gland seen within the outer region of the mesocarp and a group iso-diametric stone cells when observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
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      | caption1=Yellow oil gland seen within the outer region of the mesocarp observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
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       | description= Black pepper (fruit) (''Piper nigrum'')
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      | image2=Organic Black Pepper-1 Alkemist Laboratories.jpg
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      | caption2=Group of isodiametric stone cells observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
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      | characteristics=cellular structures identified in this botanical specimen are yellow oil gland seen within the outer region of the mesocarp and a group iso-diametric stone cells when observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
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             | reference=Pharmacopoeia of The Peoples Republic of China, Volume 1, 2005
 
             | reference=Pharmacopoeia of The Peoples Republic of China, Volume 1, 2005
 
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=HPTLC Entries=
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| {{botimg |mainimage= Organic Black Pepper Alkemist Laboratories.jpg
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          |caption1= Yellow oil gland seen within the outer region of the mesocarp observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
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          |source= Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com}}
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| {{botimg |mainimage= Organic Black Pepper-1 Alkemist Laboratories.jpg
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          |caption1= Group of isodiametric stone cells observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
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      | characteristics=cellular structures identified in this botanical specimen are yellow oil gland seen within the outer region of the mesocarp and a group iso-diametric stone cells when observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
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          |source= Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com}}
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=High Performance Thin Layer Chromatographic Identification=
 
{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
 
{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
 
             | description=Black Pepper (fruit) (''Piper nigrum'')
 
             | description=Black Pepper (fruit) (''Piper nigrum'')
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Revision as of 19:34, 18 February 2014

Contents

Nomenclature

Botanical Voucher Specimen

Organoleptic Characteristics

Macroscopic Descriptions

Microscopic Characteristics

Cellular structures identified in this botanical specimen are yellow oil gland seen within the outer region of the mesocarp and a group iso-diametric stone cells when observed at 400x with Acidified Chloral Hydrate Glycerol Solution.

Source: Elan M. Sudberg, Alkemist Laboratories [1]
AP-LOGO-Laboratories Crop - Copy.jpg


bottomright bottomright

Organic Black Pepper Alkemist Laboratories.jpg
Yellow oil gland seen within the outer region of the mesocarp observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com [2]

Organic Black Pepper-1 Alkemist Laboratories.jpg
Group of isodiametric stone cells observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com [3]

High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Piper nigrum HPTLC ID - Vanillin Sulfuric Acid Reagent UV 365 nm

Black Pepper (fruit) (Piper nigrum)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Piperine ~0.1% in CH3OH
  2. 3 μL Piper nigrum-1 (fruit)
  3. 3 μL Piper nigrum-2 (fruit)
  4. 3 μL Piper nigrum-3 (fruit)
  5. 3 μL Piper nigrum-3 (fruit)
  6. 3 μL Piper nigrum-4 (fruit)
  7. 3 μL Piper nigrum-5 (fruit)
  8. 3 μL Piperine ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase toluene: ethyl acetate [7/3] 

Sample Preparation Method 0.3g + 3mL 100% gr EtOH, sonicate/heat @ 50°C ~ 1/2 hr 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see Pharmacopoeia of The Peoples Republic of China, Volume 1, 2005


Source: Elan M. Sudberg, Alkemist Laboratories [4]

Supplementary Information

Sources

  1. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  2. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  3. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  4. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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