Hypericum perforatum (flowering tops)

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=Botanical Voucher Specimen=
 
=Botanical Voucher Specimen=
 
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          |source=Tropicos.org. Missouri Botanical Garden. 28 Feb 2014 <http://www.tropicos.org/Image/61762>}}
 
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          |source= Botanical Voucher Specimen Library, Alkemists Laboratories http://www.alkemist.com}}
 
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          |source= Botanical Voucher Specimen Library, Alkemists Laboratories http://www.alkemist.com}}
 
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           |source= Botanical Voucher Specimen Library, Alkemists Laboratories http://www.alkemist.com}}
 
           |source= Botanical Voucher Specimen Library, Alkemists Laboratories http://www.alkemist.com}}
 
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=Organoleptic Characteristics=
 
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| {{Macroscopy | source=WHO monographs on selected medicinal plants, Vol.2, World Health Organization, Geneva, 1999
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| description='''Color:''' Stem greenish-yellow to brownish-yellow; leaves greenish-grey; flowers, green sepals, golden-yellow petals
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'''Aroma/Odor:''' Weak, aromatic, balsamic
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'''Flavor/Taste:''' Bitter, acrid}}
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=Macroscopic Descriptions=
 
=Macroscopic Descriptions=
 
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Revision as of 16:47, 28 February 2014

Contents

Nomenclature

Hypericum perforatum L.   Clusiaceae  
Standardized common name (English): St John's wort

Botanical Voucher Specimen

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St. John's Wort Tropicos 61762.jpg
Source: Tropicos.org. Missouri Botanical Garden. 28 Feb 2014 <http://www.tropicos.org/Image/61762> [1]

Hypericum perforatum LF00509MMB A0153.jpg
Source: Botanical Voucher Specimen Library, Alkemists Laboratories http://www.alkemist.com [2]

Organoleptic Characteristics

Color: Stem greenish-yellow to brownish-yellow; leaves greenish-grey; flowers, green sepals, golden-yellow petals

Aroma/Odor: Weak, aromatic, balsamic Flavor/Taste: Bitter, acrid

Source: WHO monographs on selected medicinal plants, Vol.2, World Health Organization, Geneva, 1999 [3]

Macroscopic Descriptions

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Hypericum perforatum 1 RU.jpg
Hypericum perforatum growing in the field
Source: Roy Upton, American Herbal Pharmacopoeia® http://www.herbal-ahp.org/ [4]

Hypericum blossom stereo 2.jpg
Hypericum perforatum flower close-up. Note the black dots on the petals' margin only.
Source: American Herbal Pharmacopoeia® http://www.herbal-ahp.org/ [5]

Hypericum perforatum leaves stereo 1.JPG
Hypericum perforatum leaves with the characteristic black dots on the margin and translucent dots on the leaf body.
Source: American Herbal Pharmacopoeia® http://www.herbal-ahp.org/ [6]

Hypericum perforatum stem stereo 2.JPG
Hypericum perforatum stem showing opposite ridges, a defining characteristic of the species.
Source: American Herbal Pharmacopoeia® http://www.herbal-ahp.org/ [7]


Microscopic Characteristics

Oil glands with red pigment and also of importance, a three pored pollen grain that shows smooth and faintly warted exine of Hypericum perforatum.

Source: Elan M. Sudberg, Alkemist Laboratories [8]
AP-LOGO-Laboratories Crop - Copy.jpg


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Hypericum perforatum L. -Clusiaceae--1.jpg
Oil glands with red pigment of Hypericum perforatum viewed at 400x with Acidified Chloral Hydrate Solution.
Source: Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com [9]

Hypericum perforatum L. -Clusiaceae--2.jpg
Three pored pollen grain showing smooth and faintly warted exine of Hypericum perforatum viewed at 400x with Acidified Chloral Hydrate Solution.
Source: Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com [10]

St johns wort secretory cell phytolab.jpg
St. John's Wort, Hyperici herba secretory cell
Source: Claudia Borst, PhytoLab http://www.phytolab.com/en.html [11]

St johns wort pollen grain phytolab.jpg
St. John's Wort, Hyperici herba pollen grain
Source: Claudia Borst, PhytoLab http://www.phytolab.com/en.html [12]
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St johns wort hypericin gland phytolab.jpg
St. John's Wort, Hyperici herba hypericin gland
Source: Claudia Borst, PhytoLab http://www.phytolab.com/en.html [13]

St johns wort tracheidal vessels with pitted walls phytolab.jpg
St. John's Wort, Hyperici herba tracheidal vessels with pitted walls
Source: Claudia Borst, PhytoLab http://www.phytolab.com/en.html [14]

High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Hypericum perforatum HPTLC ID - Natural Product Reagent + PEG UV 365 nm

St. John's Wort (herb) (Hypericum perforatum)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Hypericin ~ 0.1% in Methanol
  2. 3 μL Hypericum perforatum-1 (herb)
  3. 3 μL Hypericum perforatum-2 (herb)
  4. 3 μL Hypericum perforatum-3 (herb)
  5. 3 μL Hypericum perforatum-3 (herb)
  6. 3 μL Hypericum perforatum-4 (herb)
  7. 3 μL Hypericum perforatum-5 (herb)
  8. 1 μL Rutin, Caffeic Acid, Hyperoside, Chlorogenic Acid ~ 0.1% in Methanol

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase ethyl acetate: Acetic acid: HCOOH: H2O [10/1.1/1.1/2.4] 

Sample Preparation Method 0.3 g + 3 ml 70% grain EtOH sonicated + heated @ 50° C ~ 1 hr 

Detection Method Natural Product Reagent + PEG -> UV 365 nm 

Reference see British Pharmacopoeia, 2003


Source: Elan M. Sudberg, Alkemist Laboratories [15]

Supplementary Information

Sources

  1. ↑ Tropicos.org. Missouri Botanical Garden. 28 Feb 2014 <http://www.tropicos.org/Image/61762>
  2. ↑ Botanical Voucher Specimen Library, Alkemists Laboratories http://www.alkemist.com
  3. ↑ WHO monographs on selected medicinal plants, Vol.2, World Health Organization, Geneva, 1999
  4. ↑ Roy Upton, American Herbal Pharmacopoeia® http://www.herbal-ahp.org/
  5. ↑ American Herbal Pharmacopoeia® http://www.herbal-ahp.org/
  6. ↑ American Herbal Pharmacopoeia® http://www.herbal-ahp.org/
  7. ↑ American Herbal Pharmacopoeia® http://www.herbal-ahp.org/
  8. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.Alkemist.com
  9. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  10. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  11. ↑ Claudia Borst, PhytoLab http://www.phytolab.com/en.html
  12. ↑ Claudia Borst, PhytoLab http://www.phytolab.com/en.html
  13. ↑ Claudia Borst, PhytoLab http://www.phytolab.com/en.html
  14. ↑ Claudia Borst, PhytoLab http://www.phytolab.com/en.html
  15. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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