Ginkgo biloba (leaf)

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=Introduction=
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{{DISPLAYTITLE:''Achillea millefolium'' (flower)}}
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=Nomenclature=
  
''Introduction from Wikipedia, the free encyclopedia (http://en.wikipedia.org/wiki/Ginkgo_biloba, retrieved 02/20/2012).''
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=Botanical Voucher Specimen=
  
Ginkgo (''Ginkgo biloba''; in Chinese and Japanese 銀杏, pinyin romanization: yín xìng, Hepburn romanization: ichō or ginnan), also spelled gingko and known as the Maidenhair Tree, is a unique species of tree with no close living relatives. The tree is widely cultivated and introduced, since an early period in human history, and has various uses as a food and traditional medicine.
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| {{botcon |companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL= http://www.alkemist.com}} || {{botcon |companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL= http://www.alkemist.com}} || {{botcon |companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL= http://www.alkemist.com}}
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| {{botimg |mainimage= Ginkgo biloba X28004BMX1 A0138.jpg
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          |source= Botanical Voucher Specimen Library, Alkemists Laboratories http://www.alkemist.com}}
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| {{botimg |mainimage= Ginkgo biloba X28004BMX3 A0462.jpg
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          |source= Botanical Voucher Specimen Library, Alkemists Laboratories http://www.alkemist.com}}
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| {{botimg |mainimage= Ginkgo biloba XO1405JD1 A0463.jpg
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          |source= Botanical Voucher Specimen Library, Alkemists Laboratories http://www.alkemist.com}}
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Ginkgos are large trees, normally reaching a height of 20–35 m (66–115 feet), with some specimens in China being over 50 m (164 feet). The tree has an angular crown and long, somewhat erratic branches, and is usually deep rooted and resistant to wind and snow damage. Young trees are often tall and slender, and sparsely branched; the crown becomes broader as the tree ages. During autumn, the leaves turn a bright yellow, then fall, sometimes within a short space of time (1–15 days). A combination of resistance to disease, insect-resistant wood and the ability to form aerial roots and sprouts makes ginkgos long-lived, with some specimens claimed to be more than 2,500 years old.
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=Organoleptic Characteristics=
 
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Ginkgo is a relatively shade-intolerant species that (at least in cultivation) grows best in environments that are well-watered and well-drained. The species shows a preference for disturbed sites; in the "semi-wild" stands at Tian Mu Shan, many specimens are found along stream banks, rocky slopes, and cliff edges. Accordingly, Ginkgo retains a prodigious capacity for vegetative growth. It is capable of sprouting from embedded buds near the base of the trunk (lignotubers, or basal chi chi) in response to disturbances, such as soil erosion. Old individuals are also capable of producing aerial roots (chi chi) on the undersides of large branches in response to disturbances such as crown damage; these roots can lead to successful clonal reproduction upon contacting the soil. These strategies are evidently important in the persistence of Ginkgo; in a survey of the "semi-wild" stands remaining in Tian Mu Shan, 40% of the Ginkgo specimens surveyed were multi-stemmed, and few saplings were present.
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''The quoted text in this section was licensed for use under the Creative Commons ShareAlike License, version 3.0: http://creativecommons.org/licenses/by-sa/3.0/''
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=Macroscopic Entries=
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{{Macroscopy | source=Steven Yeager, Mountain Rose Herbs
 
{{Macroscopy | source=Steven Yeager, Mountain Rose Herbs
 
             | companyimage=Mrh logo.jpg
 
             | companyimage=Mrh logo.jpg
 
             | companyURL=http://www.Mountain Rose Herbs.com
 
             | companyURL=http://www.Mountain Rose Herbs.com
             | description=Ginkgo (Leaf) (''Ginkgo biloba'')
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             | description=Flavor: Slightly bitter.
 
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Aroma: Slight.
 
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             | mainimage=
 
             | mainimage=
 
             | caption1=       
 
             | caption1=       
            | flavor=Slightly bitter
 
            | aroma=Slight
 
 
             | characteristics=Dark green to yellowish green; Leaves fan-shaped with tapering cuneate base; Center of apex notched and dividing blade into distinct lobes; Petiole long, with upper surface grooved. Leaf surface glabrous without midrib, and has wrinkled appearance.            | }}
 
             | characteristics=Dark green to yellowish green; Leaves fan-shaped with tapering cuneate base; Center of apex notched and dividing blade into distinct lobes; Petiole long, with upper surface grooved. Leaf surface glabrous without midrib, and has wrinkled appearance.            | }}
  
  
{{Macroscopy | source=Botanical Voucher Specimen Library, Alkemists Laboratories
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=Macroscopic Descriptions=
            | mainimage=Ginkgo biloba X28004BMX1 A0138.jpg
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{{Macroscopy | source=Steven Yeager, Mountain Rose Herbs
             | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg
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             | companyimage=Mrh logo.jpg
             | companyURL=http://www.alkemist.com
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             | companyURL=http://www.Mountain Rose Herbs.com
            | caption1=Ginkgo Botanical Voucher Specimen - Alkemists Laboratories
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             | description=Dark green to yellowish green; Leaves fan-shaped with tapering cuneate base; Center of apex notched and dividing blade into distinct lobes; Petiole long, with upper surface grooved. Leaf surface glabrous without midrib, and has wrinkled appearance.            | }}
             | description=Ginkgo (''Ginkgo biloba'') Botanical Voucher Specimen
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=Microscopic Characteristics=
| image2=Ginkgo biloba X28004BMX3 A0462.jpg
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{| border=1
| caption2=Ginkgo Botanical Voucher Specimen - Alkemists Laboratories (2/3)
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| {{botcon |companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
| image3=Ginkgo biloba XO1405JD1 A0463.jpg
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             | companyURL= http://www.alkemist.com}} || {{botcon |companyimage= TMLogoK832X75.jpg
| caption3=Ginkgo Botanical Voucher Specimen - Alkemists Laboratories (3/3)
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             | companyURL= http://www.traditionalmedicinals.com}}
            | }}
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| {{botimg |mainimage= Ginkgo_micro_image_of_Stomata_with_large_guard_cells.jpg
=Microscopic Entries=
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          |caption1=Stomate of Ginkgo biloba leaf with large thick-walled guard cells viewed under 1000x with Acidified Chloral Hydrate solution. Stomate of Ginkgo biloba leaf has large thick-walled guard cells [and] as well contains large rosettes of Calcium Oxalate crystals.
{{Microscopy | source=Elan M. Sudberg, Alkemist Laboratories
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          |source= Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com}}
            | companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
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| {{botimg |mainimage=Ginkgo polarized ca oxalate crystal, 200x.jpg
             | companyURL=http://www.Alkemist.com
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          |caption1=Calcium oxalate crystal, polarized, 200X in glycerin:dionized water solution. Epidermis has irregularly sinuous walls, often with palisade parenchmya, stomata deeply sunken surrounded by 6-8 subsidiary cells, various sizes of calcium oxalate cluster crystals abundant.
             | mainimage=Ginkgo_micro_image_of_Stomata_with_large_guard_cells.jpg
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          |source= Amy Brush, Traditional Medicinals http://www.traditionalmedicinals.com}}
            | caption1=Stomate of Ginkgo biloba leaf with large thick-walled guard cells viewed under 1000x with Acidified Chloral Hydrate solution.
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|}
            | description=Ginkgo (leaf) ''Ginkgo biloba''
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            | characteristics=Stomate of Ginkgo biloba leaf has large thick-walled guard cells as well contains large rosettes of Calcium Oxalate crystals
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            | reference=British Pharmacopoeia, 2003
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            | }}
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{{Microscopy | source=Amy Brush, Traditional Medicinals
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            | companyimage=TMLogoK832X75.jpg
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            | companyURL=http://www.traditionalmedicinals.com
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            | mainimage=Ginkgo polarized ca oxalate crystal, 200x.jpg
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            | description=Ginkgo calcium oxalate crystal (leaf) (''Ginkgo biloba'')
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            | caption1=Calcium oxalate crystal, polarized, 200X in glycerin:dionized water solution.
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            | characteristics=epidermis has irregularly sinuous walls, often with palisade parenchmya, stomata deeply sunken surrounded by 6-8 subsidiary cells, various sizes of calcium oxalate cluster crystals abundant| }}
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=HPTLC Entries=
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=High Performance Thin Layer Chromatographic Identification=
 
{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
 
{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
 
             | description=Ginkgo (leaf) ''Ginkgo biloba''
 
             | description=Ginkgo (leaf) ''Ginkgo biloba''
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             | }}
 
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=Other Points of Interest=
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=Supplementary Information=
 
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{{Botanical  | source=AHPA Known Adulterants
 
{{Botanical  | source=AHPA Known Adulterants
 
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             | adulterants=Ginkgo (''Ginkgo biloba'') leaf extract with added flavonol glycosides or aglycones (e.g., rutin, quercetin, etc.).
 
             | adulterants=Ginkgo (''Ginkgo biloba'') leaf extract with added flavonol glycosides or aglycones (e.g., rutin, quercetin, etc.).
 
               }}
 
               }}
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=Sources=
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<references />
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[[Category:Botanical]][[Categoy:Microscopy]]

Revision as of 18:57, 14 February 2014

Contents

Nomenclature

Botanical Voucher Specimen

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Ginkgo biloba X28004BMX1 A0138.jpg
Source: Botanical Voucher Specimen Library, Alkemists Laboratories http://www.alkemist.com [1]

Ginkgo biloba X28004BMX3 A0462.jpg
Source: Botanical Voucher Specimen Library, Alkemists Laboratories http://www.alkemist.com [2]

Ginkgo biloba XO1405JD1 A0463.jpg
Source: Botanical Voucher Specimen Library, Alkemists Laboratories http://www.alkemist.com [3]

Organoleptic Characteristics

Dark green to yellowish green; Leaves fan-shaped with tapering cuneate base; Center of apex notched and dividing blade into distinct lobes; Petiole long, with upper surface grooved. Leaf surface glabrous without midrib, and has wrinkled appearance.

Source: Steven Yeager, Mountain Rose Herbs [4]

link=http://www.Mountain Rose Herbs.com


Macroscopic Descriptions

Dark green to yellowish green; Leaves fan-shaped with tapering cuneate base; Center of apex notched and dividing blade into distinct lobes; Petiole long, with upper surface grooved. Leaf surface glabrous without midrib, and has wrinkled appearance.

Source: Steven Yeager, Mountain Rose Herbs [5]

link=http://www.Mountain Rose Herbs.com

Microscopic Characteristics

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Ginkgo micro image of Stomata with large guard cells.jpg
Stomate of Ginkgo biloba leaf with large thick-walled guard cells viewed under 1000x with Acidified Chloral Hydrate solution. Stomate of Ginkgo biloba leaf has large thick-walled guard cells [and] as well contains large rosettes of Calcium Oxalate crystals.
Source: Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com [6]

Ginkgo polarized ca oxalate crystal, 200x.jpg
Calcium oxalate crystal, polarized, 200X in glycerin:dionized water solution. Epidermis has irregularly sinuous walls, often with palisade parenchmya, stomata deeply sunken surrounded by 6-8 subsidiary cells, various sizes of calcium oxalate cluster crystals abundant.
Source: Amy Brush, Traditional Medicinals http://www.traditionalmedicinals.com [7]

High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Ginkgo biloba HPTLC UV 365 nm

Ginkgo (leaf) Ginkgo biloba

Lane Assignments Lane 1(3μl) Ginkgo biloba (leaf) (Vouchered Sample); Lane 2(3μl) Sophora japonica (fruit); Lane 3(3μl) Ginkgo biloba (leaf); Lane 4(3μl) Ginkgo biloba (leaf); Lane 5(3μl) Ginkgo biloba (leaf); Lane 6(3μl) Ginkgo biloba (leaf) (USA); Lane 7(3μl) Ginkgo biloba (leaf) (China) authenticated by macroscopic, microscopic &/or TLC studies according to the reference sources cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase ethyl acetate: glacial acetic acid: formic acid: water [10/1.1/1.1/2.4] 

Sample Preparation Method 0.3 g + 3 ml 100% grain EtOH sonicated + heated @ 50° C ~ 1 hr. 

Detection Method Natural Product Reagent + PEG -> UV 365 nm 

Reference see British Pharmacopoeia, 2003


Source: Elan M. Sudberg, Alkemist Laboratories [8]

Supplementary Information

AHPA Logo.gif

Ginkgo (leaf extract) (Ginkgo biloba) standardized to flavonol glycosides and terpenes. 

General Characteristics AHPA recommends in its Known Adulterants list that appropriate steps be taken to assure that this raw material is free of the noted adulterant. Contact AHPA for additional information regarding relevant analytical methods or follow this link for more information. 

Reported Adulterants Ginkgo (Ginkgo biloba) leaf extract with added flavonol glycosides or aglycones (e.g., rutin, quercetin, etc.).

Source: AHPA Known Adulterants [9]

Sources

  1. Botanical Voucher Specimen Library, Alkemists Laboratories http://www.alkemist.com
  2. Botanical Voucher Specimen Library, Alkemists Laboratories http://www.alkemist.com
  3. Botanical Voucher Specimen Library, Alkemists Laboratories http://www.alkemist.com
  4. Steven Yeager, Mountain Rose Herbs http://www.Mountain Rose Herbs.com
  5. Steven Yeager, Mountain Rose Herbs http://www.Mountain Rose Herbs.com
  6. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  7. Amy Brush, Traditional Medicinals http://www.traditionalmedicinals.com
  8. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  9. AHPA Known Adulterants http://www.ahpa.org/

Categoy:Microscopy

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