Piper nigrum (fruit)

Microscopic Characteristics

Cellular structures identified in this botanical specimen are yellow oil gland seen within the outer region of the mesocarp and a group iso-diametric stone cells when observed at 400x with Acidified Chloral Hydrate Glycerol Solution. Source: Elan M. Sudberg, Alkemist Laboratories Yellow oil gland seen within the outer region of the mesocarp observed at 400x with Acidified Chloral Hydrate Glycerol Solution. Source: Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com Group of isodiametric stone cells observed at 400x with Acidified Chloral Hydrate Glycerol Solution. Source: Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com

High Performance Thin Layer Chromatographic Identification

Piper nigrum HPTLC ID - Vanillin Sulfuric Acid Reagent UV 365 nm Black Pepper (fruit) (Piper nigrum) Lane Assignments Lanes, from left to right (Track, Volume, Sample): 3 μL Piperine ~0.1% in CH3OH 3 μL Piper nigrum-1 (fruit) 3 μL Piper nigrum-2 (fruit) 3 μL Piper nigrum-3 (fruit) 3 μL Piper nigrum-3 (fruit) 3 μL Piper nigrum-4 (fruit) 3 μL Piper nigrum-5 (fruit) 3 μL Piperine ~0.1% in CH3OH Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA.  Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates  Mobile Phase toluene: ethyl acetate [7/3]  Sample Preparation Method 0.3g + 3mL 100% gr EtOH, sonicate/heat @ 50°C ~ 1/2 hr  Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm  Reference see Pharmacopoeia of The Peoples Republic of China, Volume 1, 2005 Source: Elan M. Sudberg, Alkemist Laboratories