Source: Botanical Voucher Specimen Library, Alkemists Laboratories^[1]

Source: Royal Botanic Gardens, Kew.^[2]

Source: Tropicos.org. Missouri Botanical Garden. 05 Aug 2013^[4]

Source: PlantaPhile^[5]

Source: PlantaPhile^[6]

Large thin-walled parenchyma showing intercellular spaces of Aesculus hippocastanum seed viewed at 400x with Acidified Chloral Hydrate Solution.
Source: Elan M. Sudberg, Alkemist Laboratories^[7]

Starch granules showing well marked cleft of Aesculus hippocastanum seed viewed at 400x with Acidified Chloral Hydrate Solution..
Source: Elan M. Sudberg, Alkemist Laboratories^[8]

Horse Chestnut HPTLC ID - Anisaldehyde Reagent, White RT.

Horse Chestnut (seed) (Aesculus hippocastanum)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

1. 10 μL Escin
2. 10 μL Horse Chestnut whole 1
3. 10 μL Horse Chestnut powder
4. 12 μL Horse Chestnut whole 2 

Reference Sample(s) Reference: Dissolve 15 mg of escin in 3 mL of methanol. 

Stationary Phase Stationary phase, i.e. Silica gel 60, F254 

Mobile Phase 1-butanol, water, glacial acetic acid 50:40:10 (v/v/v) 

Sample Preparation Method Sample: Mix 1 g of powdered sample with 10 mL of ethanol-water (7:3), heat on a steam bath for 10 minutes, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions

Anisaldehyde Reagent Preparation: 1mL anisaldehyde reagent, 20mL acetic acid 99%, 170mL methanol, 10ml sulfuric acid 95%-97% 

Detection Method Anisaldehyde Reagent Use: Dip (time 0, speed 5), heat at 100°C for 4 min 

Other Notes Compare result under white RT with reference images in Image Comparison Viewer. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present.

A. hippocastanum Identification: Above the zone due to escin the chromatogram shows several narrow, brown to brownish-red zones that are less intense than the zone corresponding to escin.

Source: CAMAG HPTLC ^[9]