Althaea officinalis (root)

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Contents

Introduction

Macroscopic Entries

Marshmallow (Althaea officinalis

Reference see Tropicos.org. Missouri Botanical Garden. 06 Aug 2013 <http://www.tropicos.org/Image/100107288>, Tropicos.org. Missouri Botanical Garden. 06 Aug 2013 <http://www.tropicos.org/Image/100107287>

Source: MOBOT, Tropicos.org [1]

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Marshmallow (Althaea officinalis)

Microscopic Entries

Bast fibers observed at 400x with Acidified Chloral Hydrate Glycerol Solution.cellular structures identified in this botanical specimen are bast fibers and the mucilage bearing parenchyma cells with a rosette of calcium oxalate when observed at 400x with Acidified Chloral Hydrate Glycerol Solution.

Source: Elan M. Sudberg, Alkemist Laboratories [2]

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Marshmallow Alkemist Laboratories.jpg



HPTLC Entries

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Althaea officinalis HPTLC ID - Natural Product Reagent + PEG UV 365 nm

Marshmallow (root) (Althaea officinalis)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 1 μL Caffeic acid, Chlorogenic Acid, Hyperoside, Rutin ~0.1% in CH3OH
  2. 3 μL Althaea officinalis-1 (root)
  3. 3 μL Althaea officinalis-2 (root)
  4. 3 μL Althaea officinalis-3 (root)
  5. 3 μL Althaea officinalis-3 (root)
  6. 3 μL Althaea officinalis-4 (root)
  7. 3 μL Althaea officinalis-5 (root)
  8. 1 μL Caffeic acid, Chlorogenic Acid, Hyperoside, Rutin ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase ethyl acetate: glacial acetic acid: formic acid: water [10/1.1/1.1/2.4] 

Sample Preparation Method 0.5 g + 5 mL MeOH sonicate/heat @ 50°C ~ 1/2 hr, cntrfg, evap w N2, qs 1mL MeOH 

Detection Method Natural Product Reagent + PEG -> UV 365 nm 

Reference see British Herbal Pharmacopoeia, 1996


Source: Elan M. Sudberg, Alkemist Laboratories [3]

Other Points of Interest


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