Calendula officinalis (flower)

From AHPA Botanical Identity References Compendium
(Difference between revisions)
Jump to: navigation, search
m (Italicized scientific name)
Line 16: Line 16:
  
 
=HPTLC Entries=
 
=HPTLC Entries=
 +
{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
 +
            | description=Marigold (flower) (''Calendula officinalis'')
 +
            | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg
 +
            | companyURL=http://www.alkemist.com
 +
            | mainimage=Calendula_officinalis_-_Alkemists_Laboratories.jpg
 +
            | caption1=''Calendula officinalis'' HPTLC ID - Natural Product Reagent + PEG UV 365 nm
 +
            | stationaryphase=Silica gel 60, F254, 10 x 10 cm HPTLC plates
 +
            | mobilephase=ethyl acetate: HCOOH: Acetic acid: H2O [10/1.1/1.1/2.4]
 +
            | prep=0.3 g + 3 ml CH3OH sonicate/heat @ 50° C ~ 1/2 hr.
 +
            | detection=Natural Product Reagent + PEG -> UV 365 nm
 +
            | lanes= Lanes, from left to right (Track, Volume, Sample):
 +
# 1 μL Rutin, Caffeic Acid, Hyperoside, Chlorogenic Acid ~ 0.1% in Methanol
 +
# 3 μL ''Calendula officinalis''-1 (flower)
 +
# 3 μL ''Calendula officinalis''-2 (flower)
 +
# 3 μL ''Calendula officinalis''-3 (flower)
 +
# 3 μL ''Calendula officinalis''-3 (flower)
 +
# 3 μL ''Calendula officinalis''-4 (flower)
 +
# 3 μL ''Calendula officinalis''-5 (flower)
 +
# 1 μL Rutin, Caffeic Acid, Hyperoside, Chlorogenic Acid ~ 0.1% in Methanol
 +
 +
Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.
 +
 +
            | reference=Plant Drug Analysis, Wagner, H., 1996
 +
            | }}
 +
 
=Other Points of Interest=
 
=Other Points of Interest=
 
[[Category:blank]]
 
[[Category:blank]]

Revision as of 23:45, 1 December 2012

Contents

Introduction

Macroscopic Entries

Microscopic Entries

Large spherical pollen grain showing sharp spiny exine with three pores from Calendula officinalis flower viewed at 400x with Acidified Chloral Hydrate Glycerol Solution.cellular structures identified in Calendula officinalis flower are the large spherical pollen grain showing sharp spiny exine with three pores and a fibrous layer of anther in surface view when observed at 400x with Acidified Chloral Hydrate Glycerol Solution.

Source: Elan M. Sudberg, Alkemist Laboratories [1]

AP-LOGO-Laboratories Crop - Copy.jpg
Calendula officinalis L. -Asteraceae--1.jpg



HPTLC Entries

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Calendula officinalis HPTLC ID - Natural Product Reagent + PEG UV 365 nm

Marigold (flower) (Calendula officinalis)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 1 μL Rutin, Caffeic Acid, Hyperoside, Chlorogenic Acid ~ 0.1% in Methanol
  2. 3 μL Calendula officinalis-1 (flower)
  3. 3 μL Calendula officinalis-2 (flower)
  4. 3 μL Calendula officinalis-3 (flower)
  5. 3 μL Calendula officinalis-3 (flower)
  6. 3 μL Calendula officinalis-4 (flower)
  7. 3 μL Calendula officinalis-5 (flower)
  8. 1 μL Rutin, Caffeic Acid, Hyperoside, Chlorogenic Acid ~ 0.1% in Methanol

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase ethyl acetate: HCOOH: Acetic acid: H2O [10/1.1/1.1/2.4] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicate/heat @ 50° C ~ 1/2 hr. 

Detection Method Natural Product Reagent + PEG -> UV 365 nm 

Reference see Plant Drug Analysis, Wagner, H., 1996


Source: Elan M. Sudberg, Alkemist Laboratories [2]

Other Points of Interest


Cite error: <ref> tags exist, but no <references/> tag was found

Personal tools
MediaWiki Appliance - Powered by TurnKey Linux