Revision as of 06:37, 23 January 2013 by
JKim (Talk)
Introduction
Macroscopic Entries
Microscopic Entries
Large spherical pollen grain showing sharp spiny exine with three pores from Calendula officinalis flower viewed at 400x with Acidified Chloral Hydrate Glycerol Solution.cellular structures identified in Calendula officinalis flower are the large spherical pollen grain showing sharp spiny exine with three pores and a fibrous layer of anther in surface view when observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories [1]
|
|
|
|
HPTLC Entries
Calendula officinalis HPTLC ID - Natural Product Reagent + PEG UV 365 nm
Marigold (flower) (Calendula officinalis)
Lane Assignments Lanes, from left to right (Track, Volume, Sample):
- 1 μL Rutin, Caffeic Acid, Hyperoside, Chlorogenic Acid ~ 0.1% in Methanol
- 3 μL Calendula officinalis-1 (flower)
- 3 μL Calendula officinalis-2 (flower)
- 3 μL Calendula officinalis-3 (flower)
- 3 μL Calendula officinalis-3 (flower)
- 3 μL Calendula officinalis-4 (flower)
- 3 μL Calendula officinalis-5 (flower)
- 1 μL Rutin, Caffeic Acid, Hyperoside, Chlorogenic Acid ~ 0.1% in Methanol
Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.
Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates
Mobile Phase ethyl acetate: HCOOH: Acetic acid: H2O [10/1.1/1.1/2.4]
Sample Preparation Method 0.3 g + 3 ml CH3OH sonicate/heat @ 50° C ~ 1/2 hr.
Detection Method Natural Product Reagent + PEG -> UV 365 nm
Reference see Plant Drug Analysis, Wagner, H., 1996
Source: Elan M. Sudberg, Alkemist Laboratories [2]
|
Other Points of Interest
Cite error: <ref>
tags exist, but no <references/>
tag was found