Camellia sinensis (leaf)
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− | + | {{DISPLAYTITLE:''Camellia sinensis'' (leaf)}} | |
− | '' | + | =Nomenclature= |
− | + | =Botanical Voucher Specimen= | |
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+ | | {{botcon |companyimage= TropicosLogo.gif | ||
+ | | companyURL= http://www.tropicos.org/Name/31600230 }} || {{botcon |companyimage= TropicosLogo.gif | ||
+ | | companyURL= http://www.tropicos.org/Name/31600230}} || {{botcon |companyimage= TropicosLogo.gif | ||
+ | | companyURL= http://www.tropicos.org/Name/31600230}} | ||
+ | |- | ||
+ | | {{botimg |mainimage=Camellia sinensis Tropicos 30076.jpg | ||
+ | |source=Tropicos.org. Missouri Botanical Garden. 13 Sep 2013 <http://www.tropicos.org/Image/30076>}} | ||
+ | | {{botimg |mainimage=Camellia sinensis Tropicos 30070.jpg | ||
+ | |source=Tropicos.org. Missouri Botanical Garden. 13 Sep 2013 <http://www.tropicos.org/Image/30070>}} | ||
+ | | {{botimg |mainimage= Camellia sinensis Tropicos 30074.jpg | ||
+ | |source=Tropicos.org. Missouri Botanical Garden. 13 Sep 2013 <http://www.tropicos.org/Image/30074>}} | ||
+ | |} | ||
− | + | =Organoleptic Characteristics= | |
− | =Macroscopic | + | =Macroscopic Descriptions= |
− | { | + | {| border=1 |
− | + | | {{botcon |companyimage= AP-LOGO-Laboratories Crop - Copy.jpg | |
− | + | | companyURL= http://www.alkemist.com}} | |
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− | + | | {{botimg |mainimage= Alkemists_Camellia_sinensis_(L.)_Kuntze_-Theaceae-_Macro.jpg | |
− | + | |caption1= Macroscopic image of cut and sifted ''Camellia sinensis'' | |
− | + | |source= Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com}} | |
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− | + | =Microscopic Characteristics= | |
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{{Microscopy | source=Greenish, H. et. al. (1908) An Anatomical Atlas of Vegetable Powders | {{Microscopy | source=Greenish, H. et. al. (1908) An Anatomical Atlas of Vegetable Powders | ||
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| characteristics=The upper epidermis is composed of cells with undulating walls and covered with a rather thick cuticle. The lower epidermis consists of smaller cells and is alone provided with stomata; the latter are surrounded by three or four tangentially elongated cells. | | characteristics=The upper epidermis is composed of cells with undulating walls and covered with a rather thick cuticle. The lower epidermis consists of smaller cells and is alone provided with stomata; the latter are surrounded by three or four tangentially elongated cells. | ||
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* The ''calcium oxalate in cluster crystals.'' }} | * The ''calcium oxalate in cluster crystals.'' }} | ||
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− | | | + | | {{botcon |companyimage= AP-LOGO-Laboratories Crop - Copy.jpg |
− | + | | companyURL= http://www.alkemist.com}} | |
− | + | | {{botcon |companyimage= AP-LOGO-Laboratories Crop - Copy.jpg | |
+ | | companyURL= http://www.alkemist.com}} | ||
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+ | | {{botimg |mainimage= 12 0206 Camellia sinensis plate from Greenish et al Anotomical Atlas.JPG | ||
+ | |source= Greenish, H. et. al. (1908) An Anatomical Atlas of Vegetable Powders}} | ||
+ | | {{botimg |mainimage= HGRNOA 18974 twin ca oxalate, 200x.jpg | ||
+ | |caption1= Twin calcium oxalate crystals, polarized. 200X glyercin : deionized water solution. | ||
+ | |source= Amy Brush, Traditional Medicinals}} | ||
+ | | {{botimg |mainimage= Alkemists_Camellia_sinensis_(L.)_Kuntze_-Theaceae-_irregular_and_branched_astrosclereid_from_the_leaf.jpg | ||
+ | |caption1= Irregular and branched astrosclereid from ''Camellia sinensis'' leaf viewed at 400x with Acidified Chloral Hydrate Soln. | ||
+ | |source= Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com}} | ||
+ | | {{botimg |mainimage= Alkemists_Camellia_sinensis_(L.)_Kuntze_-Theaceae-_thick_walled_unicellular_trichome.jpg | ||
+ | |caption1= Thick walled unicellular trichome viewed at 400x under polarized light with Acidified Chloral Hydrate. | ||
+ | |source= Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com}} | ||
+ | |} | ||
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− | + | =High Performance Thin Layer Chromatographic Identification= | |
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{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories | {{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories | ||
| description=Green Tea (leaf) (''Camellia sinensis'') | | description=Green Tea (leaf) (''Camellia sinensis'') | ||
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| reference=Herbal Drugs and Phytopharmaceuticals, Wichtl, M., 1994 | | reference=Herbal Drugs and Phytopharmaceuticals, Wichtl, M., 1994 | ||
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{{HPTLC | source=HPTLC Association | {{HPTLC | source=HPTLC Association | ||
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Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows four brownish-orange zones corresponding to reference substance epigallocatechin-3-O-gallate (Rf ~ 0.37), (-)-epigallocatechin (Rf ~ 0.46), (-)-epicatechin gallate (Rf ~ 0.52), and (-)-epicatechin (Rf ~ 0.62). The lowest zone is the most intense and the upper zone is the faintest. The two zones in between are clearly separated (black arrows). | Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows four brownish-orange zones corresponding to reference substance epigallocatechin-3-O-gallate (Rf ~ 0.37), (-)-epigallocatechin (Rf ~ 0.46), (-)-epicatechin gallate (Rf ~ 0.52), and (-)-epicatechin (Rf ~ 0.62). The lowest zone is the most intense and the upper zone is the faintest. The two zones in between are clearly separated (black arrows). | ||
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+ | =Supplementary Information= | ||
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Revision as of 17:21, 11 February 2014
Contents |
Nomenclature
Botanical Voucher Specimen
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Organoleptic Characteristics
Macroscopic Descriptions
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Microscopic Characteristics
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High Performance Thin Layer Chromatographic Identification
Green Tea (leaf) (Camellia sinensis) Lane Assignments Lanes, from left to right (Track, Volume, Sample):
Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates Mobile Phase CHCl3: ethyl formate: HCOOH [5/4/1] Sample Preparation Method 0.3 g + 3ml 70% grain EtOH sonicated + heated @ 50° C ~ 1 hr Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm Reference see Herbal Drugs and Phytopharmaceuticals, Wichtl, M., 1994
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Green Tea (leaf) (Camellia sinensis) Lane Assignments Lanes, from left to right (Track, Volume, Sample):
Reference Sample(s) Reference:Individually dissolve 1 mg of (-)-epigallocatechin and 1 mg of (-)-epicatechin gallate each in 20 mL of methanol; Optional: Individually dissolve 1 mg of (-)-epigallo-catechin-3-O-gallate and 1 mg of (-)-epicatechin each in 20 mL of methanol; Store all solutions at -20°C. Stationary Phase Stationary phase, i.e. Silica gel 60, F254 Mobile Phase Toluene, acetone, formic acid 9:9:2 (v/v/v) Sample Preparation Method Sample: Mix 100 mg of powdered sample with 10 mL of methanol, water 4:1 and sonicate for 10 minutes, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions. Derivatization reagent: Fast Blue salt B reagent; Preparation: dissolve 140 mg of Fast Blue salt B in 10 mL of water and add 140 mL of methanol and 50 mL of dichloromethane. Store reagent in the dark at 4°C; Use: preheat the plate to 100°C for 2 min, then dip (time 0, speed 5), dry for 5 min in the fume hood. Detection Method Unsaturated chamber; developing distance 60 mm from lower edge; relative humidity 33% Other Notes Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes. System suitability test: (-)-Epigallocatechin: brown zone at Rf ~ 0.46; (-)-Epicatechin gallate: brown zone at Rf ~ 0.52 Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows four brownish-orange zones corresponding to reference substance epigallocatechin-3-O-gallate (Rf ~ 0.37), (-)-epigallocatechin (Rf ~ 0.46), (-)-epicatechin gallate (Rf ~ 0.52), and (-)-epicatechin (Rf ~ 0.62). The lowest zone is the most intense and the upper zone is the faintest. The two zones in between are clearly separated (black arrows).
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Supplementary Information
Sources
- ↑ Tropicos.org. Missouri Botanical Garden. 13 Sep 2013 <http://www.tropicos.org/Image/30076>
- ↑ Tropicos.org. Missouri Botanical Garden. 13 Sep 2013 <http://www.tropicos.org/Image/30070>
- ↑ Tropicos.org. Missouri Botanical Garden. 13 Sep 2013 <http://www.tropicos.org/Image/30074>
- ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
- ↑ Greenish, H. et. al. (1908) An Anatomical Atlas of Vegetable Powders
- ↑ Greenish, H. et. al. (1908) An Anatomical Atlas of Vegetable Powders
- ↑ Amy Brush, Traditional Medicinals
- ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
- ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
- ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
- ↑ HPTLC Association http://www.hptlc-association.org/