Centella asiatica (aerial parts)

From AHPA Botanical Identity References Compendium
(Difference between revisions)
Jump to: navigation, search
Line 16: Line 16:
  
 
=HPTLC Entries=
 
=HPTLC Entries=
 +
{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
 +
            | description=Gotu Kola (aerial parts) (''Centella asiatica'')
 +
            | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg
 +
            | companyURL=http://www.alkemist.com
 +
            | mainimage=Centella_asiatica_-_Alkemist_Laboratories.jpg
 +
            | caption1=''Centella asiatica'' HPTLC ID - Vanillin Sulfuric Acid Reagent UV 365 nm
 +
            | stationaryphase=Silica gel 60, F254, 10 x 10 cm HPTLC plates
 +
            | mobilephase=chloroform: glacial acetic acid: methanol: water [6/3.2/1.2/0.8]
 +
            | prep=0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr
 +
            | detection=Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm
 +
            | lanes= Lanes, from left to right (Track, Volume, Sample):
 +
# 3 μL Asiaticoside ~0.1% in Methanol
 +
# 3 μL ''Centella asiatica''-1 (herb)
 +
# 3 μL ''Centella asiatica''-2 (aerial parts)
 +
# 3 μL ''Centella asiatica''-3 (aerial parts)
 +
# 3 μL ''Centella asiatica''-3 (aerial parts)
 +
# 3 μL ''Centella asiatica''-4 (herb)
 +
# 3 μL ''Centella asiatica''-5 (herb)
 +
# 3 μL Asiaticoside ~0.1% in Methanol
 +
 +
Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.
 +
 +
            | reference=W.H.O. Monographs Vol. #1 1999
 +
            | }}
 +
 
=Other Points of Interest=
 
=Other Points of Interest=
 
[[Category:blank]]
 
[[Category:blank]]

Revision as of 00:07, 2 December 2012

Contents

Introduction

Macroscopic Entries

Microscopic Entries

Rosettes of calcium oxalate found within the palisade cells below leaf epidermis observed at 400x with Acidified Chloral Hydrate Glycerol Solutioncellular structures identified in this botanical specimens are the rosettes of calcium oxalate found within the palisade cells below leaf epidermis and the epidermis of the leaf showing striated cuticle and numerous stomata.

Source: Elan M. Sudberg, Alkemist Laboratories [1]

AP-LOGO-Laboratories Crop - Copy.jpg
Gotu Kola-1.jpg


HPTLC Entries

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Centella asiatica HPTLC ID - Vanillin Sulfuric Acid Reagent UV 365 nm

Gotu Kola (aerial parts) (Centella asiatica)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Asiaticoside ~0.1% in Methanol
  2. 3 μL Centella asiatica-1 (herb)
  3. 3 μL Centella asiatica-2 (aerial parts)
  4. 3 μL Centella asiatica-3 (aerial parts)
  5. 3 μL Centella asiatica-3 (aerial parts)
  6. 3 μL Centella asiatica-4 (herb)
  7. 3 μL Centella asiatica-5 (herb)
  8. 3 μL Asiaticoside ~0.1% in Methanol

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase chloroform: glacial acetic acid: methanol: water [6/3.2/1.2/0.8] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see W.H.O. Monographs Vol. #1 1999


Source: Elan M. Sudberg, Alkemist Laboratories [2]

Other Points of Interest


Cite error: <ref> tags exist, but no <references/> tag was found

Personal tools
MediaWiki Appliance - Powered by TurnKey Linux