Centella asiatica HPTLC ID - Vanillin Sulfuric Acid Reagent UV 365 nm

Gotu Kola (aerial parts) (Centella asiatica)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

1. 3 μL Asiaticoside ~0.1% in Methanol
2. 3 μL Centella asiatica-1 (herb)
3. 3 μL Centella asiatica-2 (aerial parts)
4. 3 μL Centella asiatica-3 (aerial parts)
5. 3 μL Centella asiatica-3 (aerial parts)
6. 3 μL Centella asiatica-4 (herb)
7. 3 μL Centella asiatica-5 (herb)
8. 3 μL Asiaticoside ~0.1% in Methanol

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase chloroform: glacial acetic acid: methanol: water [6/3.2/1.2/0.8] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see W.H.O. Monographs Vol. #1 1999

Source: Elan M. Sudberg, Alkemist Laboratories ^[2]