Centella asiatica (aerial parts)

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=Introduction=
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{{DISPLAYTITLE:''Centella asiatica'' (aerial parts)}}
=Macroscopic Entries=
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=Nomenclature=
=Microscopic Entries=
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=Botanical Voucher Specimen=
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=Organoleptic Characteristics=
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=Macroscopic Descriptions=
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=Microscopic Characteristics=
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{{Microscopy | source=Elan M. Sudberg, Alkemist Laboratories
 
{{Microscopy | source=Elan M. Sudberg, Alkemist Laboratories
 
             | companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
 
             | companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
 
             | companyURL=http://www.Alkemist.com
 
             | companyURL=http://www.Alkemist.com
            | mainimage=Gotu Kola-1.jpg
 
            | caption1= Rosettes of calcium oxalate found within the palisade cells below leaf epidermis observed at 400x with Acidified Chloral Hydrate Glycerol Solution
 
            | description= Gotu Kola (aerial parts) (''Centella asiatica'')
 
            | image2=Gotu Kola-2.jpg
 
            | caption2= Epidermis of the leaf showing striated cuticle and numerous stomata observed at 400x with Acidified Chloral Hydrate Glycerol Solution
 
 
             | characteristics=cellular structures identified in this botanical specimens are the rosettes of calcium oxalate found within the palisade cells below leaf epidermis and the epidermis of the leaf showing striated cuticle and numerous stomata.
 
             | characteristics=cellular structures identified in this botanical specimens are the rosettes of calcium oxalate found within the palisade cells below leaf epidermis and the epidermis of the leaf showing striated cuticle and numerous stomata.
 
             | reference=British Pharmacopoeia, 2011
 
             | reference=British Pharmacopoeia, 2011
 
             | }}
 
             | }}
  
=HPTLC Entries=
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{| border=1
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| {{botcon |companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL= http://www.Alkemist.com}} || {{botcon |companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL= http://www.Alkemist.com}}
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|-
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| {{botimg |mainimage= Gotu Kola-1.jpg
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          |caption1= Rosettes of calcium oxalate found within the palisade cells below leaf epidermis observed at 400x with Acidified Chloral Hydrate Glycerol Solution
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          |source= Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com}}
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| {{botimg |mainimage= Gotu Kola-2.jpg
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          |caption1= Epidermis of the leaf showing striated cuticle and numerous stomata observed at 400x with Acidified Chloral Hydrate Glycerol Solution
 +
          |source= Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com}}
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|}
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|}
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=High Performance Thin Layer Chromatographic Identification=
 
{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
 
{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
 
             | description=Gotu Kola (aerial parts) (''Centella asiatica'')
 
             | description=Gotu Kola (aerial parts) (''Centella asiatica'')
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=Other Points of Interest=
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=Supplementary Information=
[[Category:NoIntro]]
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=Sources=
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<references />

Revision as of 18:52, 11 February 2014

Contents

Nomenclature

Botanical Voucher Specimen

Organoleptic Characteristics

Macroscopic Descriptions

Microscopic Characteristics

cellular structures identified in this botanical specimens are the rosettes of calcium oxalate found within the palisade cells below leaf epidermis and the epidermis of the leaf showing striated cuticle and numerous stomata.

Source: Elan M. Sudberg, Alkemist Laboratories [1]

AP-LOGO-Laboratories Crop - Copy.jpg


bottomright bottomright

Gotu Kola-1.jpg
Rosettes of calcium oxalate found within the palisade cells below leaf epidermis observed at 400x with Acidified Chloral Hydrate Glycerol Solution
Source: Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com [2]

Gotu Kola-2.jpg
Epidermis of the leaf showing striated cuticle and numerous stomata observed at 400x with Acidified Chloral Hydrate Glycerol Solution
Source: Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com [3]

High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Centella asiatica HPTLC ID - Vanillin Sulfuric Acid Reagent UV 365 nm

Gotu Kola (aerial parts) (Centella asiatica)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Asiaticoside ~0.1% in Methanol
  2. 3 μL Centella asiatica-1 (herb)
  3. 3 μL Centella asiatica-2 (aerial parts)
  4. 3 μL Centella asiatica-3 (aerial parts)
  5. 3 μL Centella asiatica-3 (aerial parts)
  6. 3 μL Centella asiatica-4 (herb)
  7. 3 μL Centella asiatica-5 (herb)
  8. 3 μL Asiaticoside ~0.1% in Methanol

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase chloroform: glacial acetic acid: methanol: water [6/3.2/1.2/0.8] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see W.H.O. Monographs Vol. #1 1999


Source: Elan M. Sudberg, Alkemist Laboratories [4]

Supplementary Information

Sources

  1. Elan M. Sudberg, Alkemist Laboratories http://www.Alkemist.com
  2. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  3. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  4. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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