Centella asiatica (aerial parts)

From AHPA Botanical Identity References Compendium
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           |caption1= Rosettes of calcium oxalate found within the palisade cells below leaf epidermis observed at 400x with Acidified Chloral Hydrate Glycerol Solution
 
           |caption1= Rosettes of calcium oxalate found within the palisade cells below leaf epidermis observed at 400x with Acidified Chloral Hydrate Glycerol Solution
           |source= Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com}}
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           |source= Elan M. Sudberg, Alkemist Laboratories
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           |caption1= Epidermis of the leaf showing striated cuticle and numerous stomata observed at 400x with Acidified Chloral Hydrate Glycerol Solution
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           |caption2= Epidermis of the leaf showing striated cuticle and numerous stomata observed at 400x with Acidified Chloral Hydrate Glycerol Solution
           |source= Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com}}
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           |source2= Elan M. Sudberg, Alkemist Laboratories}}
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Revision as of 01:43, 17 March 2014


Contents

Nomenclature

Centella asiatica (L.) Urb.   Apiaceae  
Syn. Hydrocotyle asiatica L.  
Standardized common name (English): gotu kola  
Ayurvedic name(s): brahmi; mandukaparni  
Pinyin name(s): ji xue cao; ji xue cao (whole plant); luo-de-da (whole plant); ma ti cao (whole plant)

Botanical Voucher Specimen

Organoleptic Characteristics

Macroscopic Characteristics

Microscopic Characteristics

Cellular structures identified in this botanical specimens are the rosettes of calcium oxalate found within the palisade cells below leaf epidermis and the epidermis of the leaf showing striated cuticle and numerous stomata.

Source: Elan M. Sudberg, Alkemist Laboratories [1]

AP-LOGO-Laboratories Crop - Copy.jpg


bottomright bottomright

Gotu Kola-1.jpg
Rosettes of calcium oxalate found within the palisade cells below leaf epidermis observed at 400x with Acidified Chloral Hydrate Glycerol Solution
Source: Elan M. Sudberg, Alkemist Laboratories[2]

Gotu Kola-2.jpg
Epidermis of the leaf showing striated cuticle and numerous stomata observed at 400x with Acidified Chloral Hydrate Glycerol Solution
Source: Elan M. Sudberg, Alkemist Laboratories[3]

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High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Centella asiatica HPTLC ID - Vanillin Sulfuric Acid Reagent UV 365 nm

Gotu Kola (aerial parts) (Centella asiatica)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Asiaticoside ~0.1% in Methanol
  2. 3 μL Centella asiatica-1 (herb)
  3. 3 μL Centella asiatica-2 (aerial parts)
  4. 3 μL Centella asiatica-3 (aerial parts)
  5. 3 μL Centella asiatica-3 (aerial parts)
  6. 3 μL Centella asiatica-4 (herb)
  7. 3 μL Centella asiatica-5 (herb)
  8. 3 μL Asiaticoside ~0.1% in Methanol

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase chloroform: glacial acetic acid: methanol: water [6/3.2/1.2/0.8] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see W.H.O. Monographs Vol. #1 1999


Source: Elan M. Sudberg, Alkemist Laboratories [4]

Supplementary Information

Sources

  1. Elan M. Sudberg, Alkemist Laboratories http://www.Alkemist.com
  2. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  3. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  4. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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