Cichorium intybus (root)

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=HPTLC Entries=
 
=HPTLC Entries=
 +
{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
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            | description=Chicory (root) (''Cichorium intybus'')
 +
            | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL=http://www.alkemist.com
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            | mainimage=Cichorium_intybus_-_Alkemists_Laboratories.jpg
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            | caption1=''Cichorium intybus'' HPTLC ID - 10% Ethanolic Sulfuric Acid Reagent UV 365 nm
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            | stationaryphase=Silica gel 60, F254, 10 x 10 cm HPTLC plates
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            | mobilephase=CH2Cl2: Petroleum ether [8/2]
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            | prep=0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr.
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            | detection=10% Ethanolic H2SO4 -> 115° C 15 min -> UV 365 nm
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            | lanes= Lanes, from left to right (Track, Volume, Sample):
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# 3 μL Oleanolic acid
 +
# 3 μL ''Cichorium intybus''-1 Vouchered Specimen (root)
 +
# 3 μL ''Cichorium intybus''-2 (root)
 +
# 3 μL ''Cichorium intybus''-3 (root)
 +
# 3 μL ''Cichorium intybus''-4 (root)
 +
# 3 μL ''Cichorium intybus''-5 (root)
 +
# 3 μL ''Cichorium intybus''-6 (root)
 +
# 3 μL Ursolic Acid
 +
 +
Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA.
 +
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            | reference=Pharmacopoeia of The Peoples Republic of China, Volume 1, 2005
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            | }}
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=Other Points of Interest=
 
=Other Points of Interest=
 
[[Category:blank]]
 
[[Category:blank]]

Revision as of 23:11, 22 October 2012

Contents

Introduction

Macroscopic Entries

Microscopic Entries

Large tracheids observed at 400x with Acidified Chloral Hydrate Glycerol Solution.

Source: Elan M. Sudberg, Alkemist Laboratories [1]

AP-LOGO-Laboratories Crop - Copy.jpg
Cichorium intybus L. - Alkemist Laboratories.jpg


HPTLC Entries

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Cichorium intybus HPTLC ID - 10% Ethanolic Sulfuric Acid Reagent UV 365 nm

Chicory (root) (Cichorium intybus)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Oleanolic acid
  2. 3 μL Cichorium intybus-1 Vouchered Specimen (root)
  3. 3 μL Cichorium intybus-2 (root)
  4. 3 μL Cichorium intybus-3 (root)
  5. 3 μL Cichorium intybus-4 (root)
  6. 3 μL Cichorium intybus-5 (root)
  7. 3 μL Cichorium intybus-6 (root)
  8. 3 μL Ursolic Acid

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase CH2Cl2: Petroleum ether [8/2] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr. 

Detection Method 10% Ethanolic H2SO4 -> 115° C 15 min -> UV 365 nm 

Reference see Pharmacopoeia of The Peoples Republic of China, Volume 1, 2005


Source: Elan M. Sudberg, Alkemist Laboratories [2]

Other Points of Interest


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