Cichorium intybus HPTLC ID - 10% Ethanolic Sulfuric Acid Reagent UV 365 nm

Chicory (root) (Cichorium intybus)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

1. 3 μL Oleanolic acid
2. 3 μL Cichorium intybus-1 Vouchered Specimen (root)
3. 3 μL Cichorium intybus-2 (root)
4. 3 μL Cichorium intybus-3 (root)
5. 3 μL Cichorium intybus-4 (root)
6. 3 μL Cichorium intybus-5 (root)
7. 3 μL Cichorium intybus-6 (root)
8. 3 μL Ursolic Acid

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase CH2Cl2: Petroleum ether [8/2] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr. 

Detection Method 10% Ethanolic H2SO4 -> 115° C 15 min -> UV 365 nm 

Reference see Pharmacopoeia of The Peoples Republic of China, Volume 1, 2005

Source: Elan M. Sudberg, Alkemist Laboratories ^[2]