Cucurbita pepo (seed)

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=Microscopic Entries=
 
=Microscopic Entries=
 
=HPTLC Entries=
 
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{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
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            | description=Pumpkin (seed) (''Cucurbita pepo'')
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            | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL=http://www.alkemist.com
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            | mainimage=Cucurbita_pepo_-_Alkemists_Laboratories.jpg
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            | caption1=''Cucurbita pepo'' HPTLC ID - Vanillin Sulfuric Acid Reagent Visible Light
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            | stationaryphase=Silica gel 60, F254, 10 x 10 cm HPTLC plates
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            | mobilephase=chloroform: ethanol [9.5/0.5]
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            | prep=0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr.
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            | detection=Vanillin/H2SO4 Reagent -> 110° C 5 min -> visible light
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            | lanes= Lanes, from left to right (Track, Volume, Sample):
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# 3 μL ''Cucurbita pepo''-1 (seed)
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# 3 μL ''Cucurbita pepo''-2 (seed no shells)
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# 3 μL ''Cucurbita pepo''-3 (seed no shells)
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# 3 μL ''Cucurbita pepo''-4 (seed)
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# 3 μL ''Cucurbita pepo''-4 (seed)
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# 3 μL ''Cucurbita pepo''-5 (seed no shells)
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# 3 μL ''Cucurbita pepo''-6 (seed with shells)
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# 2 μL ''Cucurbita pepo''-7 (seed)
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Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.
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            | reference=British Herbal Pharmacopoeia, 1996
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            | }}
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=Other Points of Interest=
 
=Other Points of Interest=
 
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[[Category:blank]]

Revision as of 23:55, 29 November 2012

Contents

Introduction

Macroscopic Entries

Microscopic Entries

HPTLC Entries

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Cucurbita pepo HPTLC ID - Vanillin Sulfuric Acid Reagent Visible Light

Pumpkin (seed) (Cucurbita pepo)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Cucurbita pepo-1 (seed)
  2. 3 μL Cucurbita pepo-2 (seed no shells)
  3. 3 μL Cucurbita pepo-3 (seed no shells)
  4. 3 μL Cucurbita pepo-4 (seed)
  5. 3 μL Cucurbita pepo-4 (seed)
  6. 3 μL Cucurbita pepo-5 (seed no shells)
  7. 3 μL Cucurbita pepo-6 (seed with shells)
  8. 2 μL Cucurbita pepo-7 (seed)

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase chloroform: ethanol [9.5/0.5] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr. 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> visible light 

Reference see British Herbal Pharmacopoeia, 1996


Source: Elan M. Sudberg, Alkemist Laboratories [1]

Other Points of Interest


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