Cucurbita pepo (seed)

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{{DISPLAYTITLE:''Cucurbita pepo'' (seed) }}
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{{DISPLAYTITLE:''Cucurbita pepo'' (seed) }} {{askbox|herb=''Cucurbita pepo''}}
 
=Nomenclature=
 
=Nomenclature=
 
 
{{nomenclature | binomial=Cucurbita pepo
 
{{nomenclature | binomial=Cucurbita pepo
 
|authority=L.
 
|authority=L.
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=Botanical Voucher Specimen=
 
=Botanical Voucher Specimen=
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{{Media      |cat=Voucher
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            | source=MOBOT, Tropicos.org
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            | mainimage=Cucurbita_pepo_subsp._texana_Tropicos_100135770.jpg
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            | companyimage=TropicosLogo.gif
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            | companyURL=http://www.tropicos.org/Image/100135770
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            | reference=Tropicos.org. Missouri Botanical Garden. 25 Mar 2014 <http://www.tropicos.org/Image/100135770>
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            | }}
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=Organoleptic Characteristics=
 
=Organoleptic Characteristics=
 
{| border=1
 
{| border=1
 
|  
 
|  
{{Macroscopy | source=United States Dispensatory (1918)
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{{Organolepsy | source=United States Dispensatory (1918)
| description=''Cucurbita Pepo'' [...] slightly odorous when contused; taste bland
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| description=[''Cucurbita Pepo'' is] slightly odorous when contused; taste bland
 
and oily. }}
 
and oily. }}
 
|}
 
|}
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|  
 
|  
 
{{Macroscopy | source=United States Dispensatory (1918)
 
{{Macroscopy | source=United States Dispensatory (1918)
| description=''Cucurbita Pepo'', or common pumpkin, is a plant almost too well
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| description=''Cucurbita Pepo'', or common pumpkin, is a plant almost too well known to need description.  The seeds are broadly elliptical or ovate, from 15 to 23 mm. in length and from 2 to 3 mm. in thickness; externally yellowish-white, very smooth, occasionally with thin, transparent fragments of adhering pulp and with a shallow groove parallel to and within 1 mm. of the margin; fracture short, seed-coat consisting of a white coriaceous outer layer and a membranous inner layer occasionally of a dark green color; embryo whitish, straight, with a small conical hypocotyl and two plano-convex cotyledons.}}
known to need description.  The seeds are broadly elliptical or ovate, from 15 to 23
+
mm. in length and from 2 to 3 mm. in thickness; externally yellowish-white,
+
very smooth, occasionally with thin, transparent fragments of
+
adhering pulp and with a shallow groove parallel to and within 1 mm.
+
of the margin; fracture short, seed-coat consisting of a white coriaceous
+
outer layer and a membranous inner layer occasionally of a dark green
+
color; embryo whitish, straight, with a small conical hypocotyl and two
+
plano-convex cotyledons...}}
+
 
|}
 
|}
 
=Microscopic Characteristics=
 
=Microscopic Characteristics=
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|  
 
|  
 
{{Macroscopy | source=United States Dispensatory (1918)
 
{{Macroscopy | source=United States Dispensatory (1918)
| description=Under the microscope, sections of [''Cucurbita Pepo''] show an outer
+
| description=Under the microscope, sections of [''Cucurbita Pepo''] show an outer epidermal layer consisting of palisade-like cells, the radial walls attaining a length of 1 mm., the outer walls being usually torn off so that it appears as though the seeds were covered with very long hairs; a sub-epidermal layer consisting- of from 5 to 12 rows of cells with slightly thickened, lignified and porous walls; a layer of strongly lignified stone cells, elliptical in outline and about 0.075 mm. in length; a single layer of small cells resembling those of the sub-epidermal layer; several rows of parenchyma cells with characteristic reticulate markings and separated from each other by large intercellular spaces; several layers of parenchyma cells, the inner layer being more or less collapsed and bounded on the inside by a single epidermal layer, the cells having rather thick walls; the perisperm cells are usually more or less collapsed and the endosperm consists of a single layer of cells filled with small aleurone grains; the cotyledons consist of thin-walled, isodiametric, elongated or palisade-like cells containing a fixed oil and numerous small aleurone grains.}}
epidermal layer consisting of palisade-like cells, the radial walls
+
attaining a length of 1 mm., the outer walls being usually torn off so
+
that it appears as though the seeds were covered with very long hairs; a
+
sub-epidermal layer consisting- of from 5 to 12 rows of cells with slightly
+
thickened, lignified and porous walls; a layer of strongly lignified stone
+
cells, elliptical in outline and about 0.075 mm. in length; a single layer
+
of small cells resembling those of the sub-epidermal layer; several rows
+
of parenchyma cells with characteristic reticulate markings and
+
separated from each other by large intercellular spaces; several layers of
+
parenchyma cells, the inner layer being more or less collapsed and
+
bounded on the inside by a single epidermal layer, the cells having
+
rather thick walls; the perisperm cells are usually more or less collapsed
+
and the endosperm consists of a single layer of cells filled with small
+
aleurone grains; the cotyledons consist of thin-walled, isodiametric,
+
elongated or palisade-like cells containing a fixed oil and numerous
+
small aleurone grains.}}
+
 
|}
 
|}
 
=High Performance Thin Layer Chromatographic Identification=
 
=High Performance Thin Layer Chromatographic Identification=

Latest revision as of 19:29, 2 June 2014

AHPA recognizes other valuable resources exist regarding the identity of Cucurbita pepo.

To submit a suggestion or contribution, please contact Merle Zimmermann.

Contents

Nomenclature

Cucurbita pepo L.   Cucurbitaceae  
Standardized common name (English): pumpkin

Botanical Voucher Specimen

bottomright

Cucurbita pepo subsp. texana Tropicos 100135770.jpg
Source: MOBOT, Tropicos.org[1]

Organoleptic Characteristics

[Cucurbita Pepo is] slightly odorous when contused; taste bland

and oily.
Source: United States Dispensatory (1918) [2]

Macroscopic Characteristics

Cucurbita Pepo, or common pumpkin, is a plant almost too well known to need description. The seeds are broadly elliptical or ovate, from 15 to 23 mm. in length and from 2 to 3 mm. in thickness; externally yellowish-white, very smooth, occasionally with thin, transparent fragments of adhering pulp and with a shallow groove parallel to and within 1 mm. of the margin; fracture short, seed-coat consisting of a white coriaceous outer layer and a membranous inner layer occasionally of a dark green color; embryo whitish, straight, with a small conical hypocotyl and two plano-convex cotyledons.

Source: United States Dispensatory (1918) [3]

Microscopic Characteristics

Under the microscope, sections of [Cucurbita Pepo] show an outer epidermal layer consisting of palisade-like cells, the radial walls attaining a length of 1 mm., the outer walls being usually torn off so that it appears as though the seeds were covered with very long hairs; a sub-epidermal layer consisting- of from 5 to 12 rows of cells with slightly thickened, lignified and porous walls; a layer of strongly lignified stone cells, elliptical in outline and about 0.075 mm. in length; a single layer of small cells resembling those of the sub-epidermal layer; several rows of parenchyma cells with characteristic reticulate markings and separated from each other by large intercellular spaces; several layers of parenchyma cells, the inner layer being more or less collapsed and bounded on the inside by a single epidermal layer, the cells having rather thick walls; the perisperm cells are usually more or less collapsed and the endosperm consists of a single layer of cells filled with small aleurone grains; the cotyledons consist of thin-walled, isodiametric, elongated or palisade-like cells containing a fixed oil and numerous small aleurone grains.

Source: United States Dispensatory (1918) [4]

High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Cucurbita pepo HPTLC ID - Vanillin Sulfuric Acid Reagent Visible Light

Pumpkin (seed) (Cucurbita pepo)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Cucurbita pepo-1 (seed)
  2. 3 μL Cucurbita pepo-2 (seed no shells)
  3. 3 μL Cucurbita pepo-3 (seed no shells)
  4. 3 μL Cucurbita pepo-4 (seed)
  5. 3 μL Cucurbita pepo-4 (seed)
  6. 3 μL Cucurbita pepo-5 (seed no shells)
  7. 3 μL Cucurbita pepo-6 (seed with shells)
  8. 2 μL Cucurbita pepo-7 (seed)

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase chloroform: ethanol [9.5/0.5] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr. 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> visible light 

Reference see British Herbal Pharmacopoeia, 1996


Source: Elan M. Sudberg, Alkemist Laboratories [5]

Supplementary Information

Sources

  1. MOBOT, Tropicos.org http://www.tropicos.org/Image/100135770
  2. United States Dispensatory (1918)
  3. United States Dispensatory (1918)
  4. United States Dispensatory (1918)
  5. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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