Cucurbita pepo (seed)

HPTLC Entries

Cucurbita pepo HPTLC ID - Vanillin Sulfuric Acid Reagent Visible Light Pumpkin (seed) (Cucurbita pepo) Lane Assignments Lanes, from left to right (Track, Volume, Sample): 3 μL Cucurbita pepo-1 (seed) 3 μL Cucurbita pepo-2 (seed no shells) 3 μL Cucurbita pepo-3 (seed no shells) 3 μL Cucurbita pepo-4 (seed) 3 μL Cucurbita pepo-4 (seed) 3 μL Cucurbita pepo-5 (seed no shells) 3 μL Cucurbita pepo-6 (seed with shells) 2 μL Cucurbita pepo-7 (seed) Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.  Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates  Mobile Phase chloroform: ethanol [9.5/0.5]  Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr.  Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> visible light  Reference see British Herbal Pharmacopoeia, 1996 Source: Elan M. Sudberg, Alkemist Laboratories