Cyathula officinalis (root)

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=HPTLC Entries=
 
=HPTLC Entries=
 +
{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
 +
            | description=Cyathula (root) (''Cyathula officinalis'')
 +
            | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL=http://www.alkemist.com
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            | mainimage=Cyathula officinalis - Alkemists Laboratories.jpg
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            | caption1= ''Cyathula officinalis'' HPTLC ID - 10% Methanolic H2SO4 365 nm
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            | stationaryphase=Silica gel 60, F254, 10 x 10 cm HPTLC plates
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            | mobilephase=CHCl3: Acetic acid: CH3OH: H2O [6/3.2/1.2/0.8]
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            | prep=0.3 g + 3 ml 70% grain EtOH sonicate + heat @ 50° C ~ 1/2 hr
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            | detection=10% Methanolic H2SO4 -> 115° C 15 min -> UV 365 nm
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            | lanes= Lanes, from left to right (Track, Volume, Sample):
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# 3 μL Sitosterol ~0.1% in CH3OH
 +
# 2 μL ''Cyathula officinalis''
 +
# 4 μL ''Cyathula officinalis''
 +
# 3 μL ''Cyathula officinalis''
 +
# 3 μL ''Cyathula officinalis''
 +
# 4 μL ''Cyathula officinalis''
 +
# 2 μL ''Cyathula officinalis''
 +
# 3 μL Escin ~0.1% in CH3OH
 +
 +
Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA.
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            | reference=Pharmacopoeia of The Peoples Republic of China, Volume 1, 2005
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            | }}
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 +
 
=Other Points of Interest=
 
=Other Points of Interest=
 
[[Category:blank]]
 
[[Category:blank]]

Revision as of 20:39, 22 October 2012

Contents

Introduction

Macroscopic Entries

Microscopic Entries

Parenchyma cell containing crystal sand with small prisms observed at 400x with Acidified Chloral Hydrate Glycerol Solution.

Source: Elan M. Sudberg, Alkemist Laboratories [1]

AP-LOGO-Laboratories Crop - Copy.jpg
Cyathula officinalis - Alkemist Laboratories.jpg


HPTLC Entries

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Cyathula officinalis HPTLC ID - 10% Methanolic H2SO4 365 nm

Cyathula (root) (Cyathula officinalis)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Sitosterol ~0.1% in CH3OH
  2. 2 μL Cyathula officinalis
  3. 4 μL Cyathula officinalis
  4. 3 μL Cyathula officinalis
  5. 3 μL Cyathula officinalis
  6. 4 μL Cyathula officinalis
  7. 2 μL Cyathula officinalis
  8. 3 μL Escin ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase CHCl3: Acetic acid: CH3OH: H2O [6/3.2/1.2/0.8] 

Sample Preparation Method 0.3 g + 3 ml 70% grain EtOH sonicate + heat @ 50° C ~ 1/2 hr 

Detection Method 10% Methanolic H2SO4 -> 115° C 15 min -> UV 365 nm 

Reference see Pharmacopoeia of The Peoples Republic of China, Volume 1, 2005


Source: Elan M. Sudberg, Alkemist Laboratories [2]


Other Points of Interest


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