Cyathula officinalis (root)

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Revision as of 22:46, 15 March 2014


Contents

Nomenclature

Cyathula officinalis K.C. Kuan   Amaranthaceae  
Standardized common name (English): cyathula  
Pinyin name(s): chuan niu xi (root)

Botanical Voucher Specimen

Organoleptic Characteristics

Macroscopic Characteristics

Microscopic Characteristics

Parenchyma cell containing crystal sand with small prisms observed at 400x with Acidified Chloral Hydrate Glycerol Solution.

Source: Elan M. Sudberg, Alkemist Laboratories [1]

AP-LOGO-Laboratories Crop - Copy.jpg
Cyathula officinalis - Alkemist Laboratories.jpg


High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Cyathula officinalis HPTLC ID - 10% Methanolic H2SO4 365 nm

Cyathula (root) (Cyathula officinalis)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Sitosterol ~0.1% in CH3OH
  2. 2 μL Cyathula officinalis
  3. 4 μL Cyathula officinalis
  4. 3 μL Cyathula officinalis
  5. 3 μL Cyathula officinalis
  6. 4 μL Cyathula officinalis
  7. 2 μL Cyathula officinalis
  8. 3 μL Escin ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase CHCl3: Acetic acid: CH3OH: H2O [6/3.2/1.2/0.8] 

Sample Preparation Method 0.3 g + 3 ml 70% grain EtOH sonicate + heat @ 50° C ~ 1/2 hr 

Detection Method 10% Methanolic H2SO4 -> 115° C 15 min -> UV 365 nm 

Reference see Pharmacopoeia of The Peoples Republic of China, Volume 1, 2005


Source: Elan M. Sudberg, Alkemist Laboratories [2]

Supplementary Information

Sources

  1. Elan M. Sudberg, Alkemist Laboratories http://www.Alkemist.com
  2. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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