Cyathula officinalis (root)

Microscopic Entries

Parenchyma cell containing crystal sand with small prisms observed at 400x with Acidified Chloral Hydrate Glycerol Solution. Source: Elan M. Sudberg, Alkemist Laboratories

HPTLC Entries

Cyathula officinalis HPTLC ID - 10% Methanolic H2SO4 365 nm Cyathula (root) (Cyathula officinalis) Lane Assignments Lanes, from left to right (Track, Volume, Sample): 3 μL Sitosterol ~0.1% in CH3OH 2 μL Cyathula officinalis 4 μL Cyathula officinalis 3 μL Cyathula officinalis 3 μL Cyathula officinalis 4 μL Cyathula officinalis 2 μL Cyathula officinalis 3 μL Escin ~0.1% in CH3OH Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.  Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates  Mobile Phase CHCl3: Acetic acid: CH3OH: H2O [6/3.2/1.2/0.8]  Sample Preparation Method 0.3 g + 3 ml 70% grain EtOH sonicate + heat @ 50° C ~ 1/2 hr  Detection Method 10% Methanolic H2SO4 -> 115° C 15 min -> UV 365 nm  Reference see Pharmacopoeia of The Peoples Republic of China, Volume 1, 2005 Source: Elan M. Sudberg, Alkemist Laboratories