Echinacea purpurea (root)

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{{DISPLAYTITLE:''Echinacea purpurea'' (root)}}
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{{DISPLAYTITLE:''Echinacea purpurea'' (root)}} {{askbox|herb=''Echinacea purpurea''}}
 
=Nomenclature=
 
=Nomenclature=
 
{{nomenclature | binomial=Echinacea purpurea
 
{{nomenclature | binomial=Echinacea purpurea
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=Botanical Voucher Specimen=
 
=Botanical Voucher Specimen=
  
{| border=1
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{{Media3    |cat=Voucher
| {{botcon |companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
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             | companyURL= http://www.alkemist.com}} || {{botcon |companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
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            | source=MOBOT, Tropicos.org
             | companyURL= http://www.alkemist.com}} || {{botcon |companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
+
            | mainimage=Echinacea_purpurea_Tropicos_89113.jpg
             | companyURL= http://www.alkemist.com}} || {{botcon |companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
+
            | companyimage=TropicosLogo.gif
             | companyURL= http://www.alkemist.com}} || {{botcon |companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
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             | companyURL=http://www.tropicos.org/Image/89113
             | companyURL= http://www.alkemist.com}}
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             | reference=Tropicos.org. Missouri Botanical Garden. 25 Mar 2014 <http://www.tropicos.org/Image/89113>
|-
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| {{botimg |mainimage= Echinacea purpurea (1) KA3502NSF1 A0104.jpg
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            | source2=Botanical Voucher Specimen Library, Alkemists Laboratories
          |source= Botanical Voucher Specimen Library, Alkemists Laboratories http://www.alkemist.com}}
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            | image2=Echinacea purpurea (1) KA3502NSF1 A0104.jpg
| {{botimg |mainimage= Echinacea purpurea (1) KA3502NSF2 A0751.jpg
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            | companyimage2=AP-LOGO-Laboratories Crop - Copy.jpg
          |source= Botanical Voucher Specimen Library, Alkemists Laboratories http://www.alkemist.com}}
+
             | companyURL2=http://www.alkemist.com
| {{botimg |mainimage= Echinacea purpurea (2) KA3502NSF1 A0429.jpg
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          |source= Botanical Voucher Specimen Library, Alkemists Laboratories http://www.alkemist.com}}
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            | source3=Botanical Voucher Specimen Library, Alkemists Laboratories
| {{botimg |mainimage= Echinacea purpurea (2) KA3502NSF2 A0753.jpg
+
            | image3=Echinacea purpurea (1) KA3502NSF2 A0751.jpg
          |source= Botanical Voucher Specimen Library, Alkemists Laboratories http://www.alkemist.com}}
+
            | companyimage3=AP-LOGO-Laboratories Crop - Copy.jpg
| {{botimg |mainimage= Echinacea purpurea (3) KA3502NSF1 A0431.jpg
+
             | companyURL3=http://www.alkemist.com
          |source= Botanical Voucher Specimen Library, Alkemists Laboratories http://www.alkemist.com}}
+
 
|}
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            | }}
 +
 
 +
{{Media3    |cat=Voucher
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 +
            | source=Botanical Voucher Specimen Library, Alkemists Laboratories
 +
            | mainimage=Echinacea purpurea (2) KA3502NSF1 A0429.jpg
 +
            | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg
 +
             | companyURL=http://www.alkemist.com
 +
 
 +
            | source2=Botanical Voucher Specimen Library, Alkemists Laboratories
 +
            | image2=Echinacea purpurea (2) KA3502NSF2 A0753.jpg
 +
            | companyimage2=AP-LOGO-Laboratories Crop - Copy.jpg
 +
            | companyURL2=http://www.alkemist.com
 +
 
 +
            | source3=Botanical Voucher Specimen Library, Alkemists Laboratories
 +
            | image3=Echinacea purpurea (3) KA3502NSF1 A0431.jpg
 +
            | companyimage3=AP-LOGO-Laboratories Crop - Copy.jpg
 +
            | companyURL3=http://www.alkemist.com
 +
 
 +
            | }}
 +
 
 +
{{Media | cat=Voucher
 +
 
 +
 
 +
| companyimage=Kewlogo.gif
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| companyURL=http://specimens.kew.org/herbarium/K001065963
 +
| mainimage=Echinacea_purpurea_Kew_imageBarcode=K001065963_669584.jpg
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| source=Royal Botanic Gardens, Kew.
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}}
  
 
=Organoleptic Characteristics=
 
=Organoleptic Characteristics=
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=Microscopic Characteristics=
 
=Microscopic Characteristics=
{| border=1
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{{Media2 |cat=Microscopy |companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
| {{botcon |companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
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             | companyURL= http://www.alkemist.com
             | companyURL= http://www.alkemist.com}} || {{botcon |companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
+
|companyimage2= AP-LOGO-Laboratories Crop - Copy.jpg
             | companyURL= http://www.alkemist.com}}
+
             | companyURL2= http://www.alkemist.com
|-
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|mainimage= Echinacea_purpurea_(L.)_Moench_-Asteraceae--1.jpg
| {{botimg |mainimage= Echinacea_purpurea_(L.)_Moench_-Asteraceae--1.jpg
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           |caption1= Oleoresin found in ''Echinacea purpurea'' root viewed under 400x with Acidified Chloral Hydrate Solution.
 
           |caption1= Oleoresin found in ''Echinacea purpurea'' root viewed under 400x with Acidified Chloral Hydrate Solution.
           |source= Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com}}
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           |source= Elan M. Sudberg, Alkemist Laboratories
| {{botimg |mainimage= Echinacea_purpurea_(L.)_Moench_-Asteraceae--2.jpg
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|image2= Echinacea_purpurea_(L.)_Moench_-Asteraceae--2.jpg
           |caption1= Fiber showing the lack of phytomelanin in ''Echinacea purpurea'' root viewed under 400x with Acidified Chloral Hydrate Solution.
+
           |caption2= Fiber showing the lack of phytomelanin in ''Echinacea purpurea'' root viewed under 400x with Acidified Chloral Hydrate Solution.
  
           |source= Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com}}
+
           |source2= Elan M. Sudberg, Alkemist Laboratories }}
|}
+
  
 
=High Performance Thin Layer Chromatographic Identification=
 
=High Performance Thin Layer Chromatographic Identification=
  
 
=Supplementary Information=
 
=Supplementary Information=
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==HPTLC method, Schicke, ''et al.'', 2014==
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'''A sensitive TLC method to identify Echinaceae pallidae radix.'''
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<blockquote>'''Abstract.'''
  
 +
In this work a fast, simple and sensitive qualitative TLC method was developed to identify Echinaceae pallidae radix and to distinguish this drug from similar ones. The TLC method is based on the lipophilic compounds of E. pallida. Three mobile phases provided good separation, e.g. toluene/ethylacetate 7 + 3 (v/v). A marker substance was found which shows a blue fluorescence at an excitation wavelength of 366 nm after detection with a spray agent containing 95 volume parts ethanol 96%, 5 parts trifluoroacetic acid 99% and zinc ions in 0.15 molar concentration. After spraying the chromatogram was heated at 110 degrees C for 7 min. This method is superior to HPLC methods to characterise mixtures of Echinacea extracts in terms of selectivity due to this post-chromatographic derivatisation and subsequent fluorescence detection.<ref>
 +
    Schicke B., Hagels H., Freudenstein J., Wätzig H. 2004. A sensitive TLC method to identify Echinaceae pallidae radix.  ''Pharmazie.'' 59(8):608-11. http://openurl.ingenta.com/content/nlm?genre=article&issn=0031-7144&volume=59&issue=8&spage=608&aulast=Schicke</ref></blockquote>
 
=Sources=
 
=Sources=
  
 
<references />
 
<references />
 
[[Category:Botanical]][[Category:Microscopy]]
 
[[Category:Botanical]][[Category:Microscopy]]

Latest revision as of 20:12, 20 August 2015

AHPA recognizes other valuable resources exist regarding the identity of Echinacea purpurea.

To submit a suggestion or contribution, please contact Merle Zimmermann.

Contents

[edit] Nomenclature

Echinacea purpurea (L.) Moench   Asteraceae  
Standardized common name (English): Echinacea purpurea

[edit] Botanical Voucher Specimen

bottomright bottomright bottomright

Echinacea purpurea Tropicos 89113.jpg
Source: MOBOT, Tropicos.org[1]

Echinacea purpurea (1) KA3502NSF1 A0104.jpg
Source: Botanical Voucher Specimen Library, Alkemists Laboratories[2]

Echinacea purpurea (1) KA3502NSF2 A0751.jpg
Source: Botanical Voucher Specimen Library, Alkemists Laboratories[3]

bottomright bottomright bottomright

Echinacea purpurea (2) KA3502NSF1 A0429.jpg
Source: Botanical Voucher Specimen Library, Alkemists Laboratories[4]

Echinacea purpurea (2) KA3502NSF2 A0753.jpg
Source: Botanical Voucher Specimen Library, Alkemists Laboratories[5]

Echinacea purpurea (3) KA3502NSF1 A0431.jpg
Source: Botanical Voucher Specimen Library, Alkemists Laboratories[6]

bottomright

Echinacea purpurea Kew imageBarcode=K001065963 669584.jpg
Source: Royal Botanic Gardens, Kew.[7]

[edit] Organoleptic Characteristics

[edit] Macroscopic Characteristics

[edit] Microscopic Characteristics

bottomright bottomright

Echinacea purpurea (L.) Moench -Asteraceae--1.jpg
Oleoresin found in Echinacea purpurea root viewed under 400x with Acidified Chloral Hydrate Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[8]

Echinacea purpurea (L.) Moench -Asteraceae--2.jpg
Fiber showing the lack of phytomelanin in Echinacea purpurea root viewed under 400x with Acidified Chloral Hydrate Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[9]

[edit] High Performance Thin Layer Chromatographic Identification

[edit] Supplementary Information

[edit] HPTLC method, Schicke, et al., 2014

A sensitive TLC method to identify Echinaceae pallidae radix.

Abstract. In this work a fast, simple and sensitive qualitative TLC method was developed to identify Echinaceae pallidae radix and to distinguish this drug from similar ones. The TLC method is based on the lipophilic compounds of E. pallida. Three mobile phases provided good separation, e.g. toluene/ethylacetate 7 + 3 (v/v). A marker substance was found which shows a blue fluorescence at an excitation wavelength of 366 nm after detection with a spray agent containing 95 volume parts ethanol 96%, 5 parts trifluoroacetic acid 99% and zinc ions in 0.15 molar concentration. After spraying the chromatogram was heated at 110 degrees C for 7 min. This method is superior to HPLC methods to characterise mixtures of Echinacea extracts in terms of selectivity due to this post-chromatographic derivatisation and subsequent fluorescence detection.[10]

[edit] Sources

  1. MOBOT, Tropicos.org http://www.tropicos.org/Image/89113
  2. Botanical Voucher Specimen Library, Alkemists Laboratories http://www.alkemist.com
  3. Botanical Voucher Specimen Library, Alkemists Laboratories http://www.alkemist.com
  4. Botanical Voucher Specimen Library, Alkemists Laboratories http://www.alkemist.com
  5. Botanical Voucher Specimen Library, Alkemists Laboratories http://www.alkemist.com
  6. Botanical Voucher Specimen Library, Alkemists Laboratories http://www.alkemist.com
  7. Royal Botanic Gardens, Kew. http://specimens.kew.org/herbarium/K001065963
  8. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  9. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  10. Schicke B., Hagels H., Freudenstein J., Wätzig H. 2004. A sensitive TLC method to identify Echinaceae pallidae radix. Pharmazie. 59(8):608-11. http://openurl.ingenta.com/content/nlm?genre=article&issn=0031-7144&volume=59&issue=8&spage=608&aulast=Schicke
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