Foeniculum vulgare (seed)

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=HPTLC Entries=
 
=HPTLC Entries=
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{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
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            | description=Fennel (seed) (''Foeniculum vulgare'')
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            | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL=http://www.alkemist.com
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            | mainimage=Foeniculum vulgare - Alkemists Laboratories.jpg
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            | caption1=''Foeniculum vulgare'' HPTLC ID - Vanillin/H2SO4 Reagent UV 365 nm
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            | stationaryphase=Silica gel 60, F254, 10 x 10 cm HPTLC plates
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            | mobilephase=toluene: ethyl acetate [9.5/0.5]
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            | prep=0.5g+5ml dichloromethane, sonicate/centrifuge/decant, evaporate to dryness with N2, qs 1.0 ml Toluene
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            | detection=Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm
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            | lanes= Lanes, from left to right (Track, Volume, Sample):
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# 1 μL Eugenol ~0.1% in CH3OH
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# 3 μL ''Foeniculum vulgare''-1 (seed)
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# 3 μL ''Foeniculum vulgare''-2 (seed)
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# 3 μL ''Foeniculum vulgare''-3 (seed)
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# 3 μL ''Foeniculum vulgare''-3 (seed)
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# 3 μL ''Foeniculum vulgare''-4 (seed)
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# 3 μL ''Foeniculum vulgare''-5 (seed)
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# 3 μL ''Foeniculum vulgare''-6 (seed)
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Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA.
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            | reference=British Pharmacopoeia, 2003
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            | }}
  
 
=Other Points of Interest=
 
=Other Points of Interest=
 
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Revision as of 20:48, 22 October 2012

Contents

Introduction

Macroscopic Entries

Microscopic Entries

Yellow fragments of a wide secretory canal of Foeniculum vulgare seed observed at 400x with Acidified Chloral Hydrate Glycerol Solution.cellular structures identified in this botanical specimen are the yellow fragments of a wide secretory canal and the cells of the mericarp from ;;Foeniculum vulgare;; seed observed at 400x with Acidified Chloral Hydrate Glycerol Solution.

Source: Elan M. Sudberg, Alkemist Laboratories [1]

AP-LOGO-Laboratories Crop - Copy.jpg
Fennel.jpg


HPTLC Entries

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Foeniculum vulgare HPTLC ID - Vanillin/H2SO4 Reagent UV 365 nm

Fennel (seed) (Foeniculum vulgare)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 1 μL Eugenol ~0.1% in CH3OH
  2. 3 μL Foeniculum vulgare-1 (seed)
  3. 3 μL Foeniculum vulgare-2 (seed)
  4. 3 μL Foeniculum vulgare-3 (seed)
  5. 3 μL Foeniculum vulgare-3 (seed)
  6. 3 μL Foeniculum vulgare-4 (seed)
  7. 3 μL Foeniculum vulgare-5 (seed)
  8. 3 μL Foeniculum vulgare-6 (seed)

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase toluene: ethyl acetate [9.5/0.5] 

Sample Preparation Method 0.5g+5ml dichloromethane, sonicate/centrifuge/decant, evaporate to dryness with N2, qs 1.0 ml Toluene 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see British Pharmacopoeia, 2003


Source: Elan M. Sudberg, Alkemist Laboratories [2]

Other Points of Interest


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