Foeniculum vulgare HPTLC ID - Vanillin/H2SO4 Reagent UV 365 nm

Fennel (seed) (Foeniculum vulgare)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

1. 1 μL Eugenol ~0.1% in CH3OH
2. 3 μL Foeniculum vulgare-1 (seed)
3. 3 μL Foeniculum vulgare-2 (seed)
4. 3 μL Foeniculum vulgare-3 (seed)
5. 3 μL Foeniculum vulgare-3 (seed)
6. 3 μL Foeniculum vulgare-4 (seed)
7. 3 μL Foeniculum vulgare-5 (seed)
8. 3 μL Foeniculum vulgare-6 (seed)

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase toluene: ethyl acetate [9.5/0.5] 

Sample Preparation Method 0.5g+5ml dichloromethane, sonicate/centrifuge/decant, evaporate to dryness with N2, qs 1.0 ml Toluene 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see British Pharmacopoeia, 2003

Source: Elan M. Sudberg, Alkemist Laboratories ^[2]