Glycine max (bean)

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=HPTLC Entries=
 
=HPTLC Entries=
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{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
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            | description=Soy (bean) (''Glycine max'')
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            | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL=http://www.alkemist.com
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            | mainimage=Glycine_max_-_Alkemists_Laboratories.jpg
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            | caption1=''Glycine max'' HPTLC ID - Vanillin Sulfuric Acid Reagent UV 365 nm
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            | stationaryphase=Silica gel 60, F254, 10 x 10 cm HPTLC plates
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            | mobilephase=ethyl acetate: Acetic acid: CH3OH: H2O [6/1.5/1.5/1]
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            | prep=0.3 g + 3 ml 70% grain EtOH sonicate/heat @ 50° C ~ 1/2 hr
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            | detection=Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm
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            | lanes= Lanes, from left to right (Track, Volume, Sample):
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# 3 μL Aescin ~0.1% in Methanol
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# 3 μL ''Glycine max''-1
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# 3 μL ''Glycine max''-2
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# 3 μL ''Glycine max''-3
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# 3 μL ''Glycine max''-3
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# 3 μL ''Glycine max''-4
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# 3 μL ''Glycine max''-5
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# 3 μL Daidzein ~0.1% in Methanol
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 +
Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.
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            | reference=Method Developed by Alkemists Laboratories
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            | }}
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=Other Points of Interest=
 
=Other Points of Interest=
 
[[Category:blank]]
 
[[Category:blank]]

Revision as of 00:09, 30 November 2012

Contents

Introduction

Macroscopic Entries

Microscopic Entries

Conical/dumbbell shaped (of various sizes) unicellular thick-walled fiber observed at 400x with Acidified Chloral Hydrate Glycerol Solution.cellular structures identified in this botanical specimen are conical/dumbbell shaped (of various sizes) unicellular thick-walled fiber and the prisms of calcium oxalate when observed at 400x with Acidified Chloral Hydrate Glycerol Solution.

Source: Elan M. Sudberg, Alkemist Laboratories [1]

AP-LOGO-Laboratories Crop - Copy.jpg
Soy Bean.jpg



HPTLC Entries

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Glycine max HPTLC ID - Vanillin Sulfuric Acid Reagent UV 365 nm

Soy (bean) (Glycine max)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Aescin ~0.1% in Methanol
  2. 3 μL Glycine max-1
  3. 3 μL Glycine max-2
  4. 3 μL Glycine max-3
  5. 3 μL Glycine max-3
  6. 3 μL Glycine max-4
  7. 3 μL Glycine max-5
  8. 3 μL Daidzein ~0.1% in Methanol

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase ethyl acetate: Acetic acid: CH3OH: H2O [6/1.5/1.5/1] 

Sample Preparation Method 0.3 g + 3 ml 70% grain EtOH sonicate/heat @ 50° C ~ 1/2 hr 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see Method Developed by Alkemists Laboratories


Source: Elan M. Sudberg, Alkemist Laboratories [2]

Other Points of Interest


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